Goat Anti-POLL Polyclonal Antibody – Cat# MBS241954

Anti-POLL antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

POLL Antibody – Goat Polyclonal

Name: POLL Antibody; Polyclonal; Goat; MyBioSource
Brand: MyBioSource
SKU: MBS241954
Price: 625 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241954

MBS241954 is an antibody targeting POLL. Also known as Goat Polyclonal to Human POLL / DNA Polymerase Lambda. Reactive in Bat, Gibbon, Bovine, Gorilla, Hamster, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, RatPredicted Reactivity: Dog, Chicken (at least 90%… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

POLL / DNA Polymerase Lambda, Polyclonal Antibody

Full Product Name

Goat Polyclonal to Human POLL / DNA Polymerase Lambda

Product Gene Name

anti-POLL antibody

Product Synonym Names

Anti-POLL / DNA Polymerase Lambda Antibody (C-Terminus) IHC-plus; POLL; DNA polymerase beta-2; DNA polymerase beta-N; DNA polymerase lambda; DNA polymerase kappa; Pol beta2; BETAN; Pol Lambda; POLKAPPA; Human POLL; DNA Polymerase Lambda

Clonality

Polyclonal

Host

Goat

Species Reactivity

Bat, Gibbon, Bovine, Gorilla, Hamster, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat
Predicted Reactivity: Dog, Chicken (at least 90% immunogen sequence identity)

Form/Format

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

Purity/Purification

Immunoaffinity Purified

Concentration

0.5 mg/ml (lot specific)

Specificity

Human POLL. It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.

Preparation and Storage

Store at -20 degree C. Minimize freezing and thawing.

Molecular Weight

33,839 Da

OMIM

606343

3D Structure

ModBase 3D Structure for Q9UGP5

Target Species

Human

Immunogen Description

Synthetic peptide C-LGLPYREPAERDW from the C-terminus of human POLL (NP_037406.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Pig (100%).

Immunogen Type

Synthetic peptide

Immunogen

POLL / DNA Polymerase Lambda antibody was raised against synthetic peptide C-LGLPYREPAERDW from the C-terminus of human POLL (NP_037406.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Hamster, Elephant, Panda, Rabbit, Pig (100%); Dog, Opossum, Turkey, Chicken (92%); Xenopus, Zebrafish, Sea anemone (85%).

Antigen Modification

C-Terminus

Applications Tested/Suitable for anti-POLL antibody

Immunohistochemistry (IHC - Paraffin), ELISA (EIA)

Application Notes for anti-POLL antibody

ELISA (1:32000), IHC-P (5 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Other Notes

Small volumes of anti-POLL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POLL. It may not necessarily be applicable to this product.

NCBI GI #

7019491

NCBI GeneID

27343

NCBI Accession #

NP_037406.1

UniProt Primary Accession #

Q9UGP5

UniProt Secondary Accession #

Q5JQP5; Q6NUM2; Q9BTN8; Q9HA10; Q9HB35; D3DR76

UniProt Related Accession #

Q9UGP5

GenBank Nucleotide #

NM_013274.3

NCBI Official Full Name

DNA polymerase lambda isoform a

NCBI Official Synonym Full Names

polymerase (DNA directed), lambda

NCBI Official Symbol

POLL

NCBI Official Synonym Symbols

BETAN; POLKAPPA

UniProt Protein Name

DNA polymerase lambda

UniProt Synonym Protein Names

DNA polymerase beta-2; Pol beta2; DNA polymerase kappa

Protein Family

DNA polymerase

UniProt Gene Name

POLL

UniProt Synonym Gene Names

Pol Lambda; Pol beta2

UniProt Entry Name

DPOLL_HUMAN

NCBI Protein Information

DNA polymerase lambda; DNA polymerase kappa; DNA polymerase beta-2; DNA polymerase beta-N

NCBI Summary for POLL

This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]

UniProt Comments for POLL

POLL: Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. Belongs to the DNA polymerase type-X family.

Protein type: DNA repair, damage; Transferase; EC 2.7.7.7; Lyase

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; lyase activity; DNA-directed DNA polymerase activity

Biological Process: nucleotide-excision repair; somatic hypermutation of immunoglobulin genes; DNA replication

Research Articles on POLL

1. Structural basis for the binding and incorporation of nucleotide analogs with L-stereochemistry by human DNA polymerase lambda.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POLL antibody

Reference Product	PubMed Publications
FBXW4 antibody	>4 publications with POLL and FBXW4
XRCC5 antibody	>1 publications with POLL and XRCC5
PCNA antibody	>1 publications with POLL and PCNA

Pathways associated with anti-POLL antibody

Products by Pathway	Pathway Diagram
BER Complex Pathway antibodies	BER Complex Pathway Diagram
BER Complex Pathway antibodies	BER Complex Pathway Diagram
Base Excision Repair Pathway antibodies	Base Excision Repair Pathway Diagram
Base Excision Repair Pathway antibodies	Base Excision Repair Pathway Diagram
Non-homologous End-joining Pathway antibodies	Non-homologous End-joining Pathway Diagram
Non-homologous End-joining Pathway antibodies	Non-homologous End-joining Pathway Diagram

Diseases associated with anti-POLL antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>383 publications with POLL and Inflammation
Nervous System Diseases Antibodies	>211 publications with POLL and Nervous System Diseases
Necrosis Antibodies	>170 publications with POLL and Necrosis
Skin Diseases Antibodies	>73 publications with POLL and Skin Diseases
Liver Diseases Antibodies	>63 publications with POLL and Liver Diseases
Heart Diseases Antibodies	>60 publications with POLL and Heart Diseases
Uterine Neoplasms Antibodies	>37 publications with POLL and Uterine Neoplasms
Weight Gain Antibodies	>33 publications with POLL and Weight Gain
Uterine Cervical Neoplasms Antibodies	>32 publications with POLL and Uterine Cervical Neoplasms
Kidney Diseases Antibodies	>31 publications with POLL and Kidney Diseases

Organs/Tissues associated with anti-POLL antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>572 publications with POLL and Blood
Lung Antibodies	>255 publications with POLL and Lung
Liver Antibodies	>140 publications with POLL and Liver
Brain Antibodies	>124 publications with POLL and Brain
Bone Antibodies	>101 publications with POLL and Bone
Vascular Antibodies	>90 publications with POLL and Vascular
Heart Antibodies	>77 publications with POLL and Heart
Muscle Antibodies	>68 publications with POLL and Muscle
Kidney Antibodies	>68 publications with POLL and Kidney
Skin Antibodies	>42 publications with POLL and Skin