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IFT74 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS241962

MBS241962 is an antibody targeting IFT74. Also known as Goat Polyclonal to Human IFT74 / CCDC2. Reactive in Gorilla, Human, Monkey, OrangutanPredicted Reactivity: Gibbon (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IFT74 / CCDC2, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human IFT74 / CCDC2
Product Gene Name
Product Synonym Gene Name
CCDC2
Product Synonym Names
Anti-IFT74 / CCDC2 Antibody (C-Terminus) IHC-plus; IFT74; CCDC2; CMG-1; CMG1; Human IFT74; CCDC2
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gorilla, Human, Monkey, Orangutan
Predicted Reactivity: Gibbon (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human IFT74.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
42,332 Da
OMIM
608040
3D Structure
ModBase 3D Structure for Q96LB3
Target Species
Human
Immunogen Description
Synthetic peptide C-KTIVDALHSTSGN from the C-terminus of human IFT74 (NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset (100%); Gibbon (92%); Rabbit (85%).
Immunogen Type
Synthetic peptide
Immunogen
IFT74 / CCDC2 antibody was raised against synthetic peptide C-KTIVDALHSTSGN from the C-terminus of human IFT74 (NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset (100%); Gibbon (92%); Rabbit (85%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-IFT74 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-IFT74 antibody
ELISA (1:16000), IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-IFT74 antibody

Anti-IFT74 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-IFT74 Polyclonal Antibody – Cat# MBS241962

Western Blot (WB) of anti-IFT74 antibody

Antibody staining (0.3 ug/ml) of 293 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Goat Anti-IFT74 Polyclonal Antibody, Western Blot (WB) – Cat# MBS241962

Other Notes

Small volumes of anti-IFT74 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IFT74. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
intraflagellar transport protein 74 homolog isoform a
NCBI Official Synonym Full Names
intraflagellar transport 74
NCBI Official Symbol
IFT74
NCBI Official Synonym Symbols
CMG1; CCDC2; CMG-1
UniProt Protein Name
Intraflagellar transport protein 74 homolog
UniProt Synonym Protein Names
Capillary morphogenesis gene 1 protein; CMG-1; Coiled-coil domain-containing protein 2
UniProt Gene Name
IFT74
UniProt Synonym Gene Names
CCDC2; CMG1; CMG-1
UniProt Entry Name
IFT74_HUMAN
NCBI Protein Information
intraflagellar transport protein 74 homolog; coiled-coil domain containing 2; capillary morphogenesis protein 1; intraflagellar transport 74 homolog; capillary morphogenesis gene 1 protein; coiled-coil domain-containing protein 2

UniProt Comments for IFT74

IFT74: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 9p21.2

Cellular Component: centrosome; cytoplasmic membrane-bound vesicle; nucleus; cilium

Molecular Function: beta-tubulin binding; chromatin binding

Biological Process: Notch signaling pathway; organelle organization and biogenesis; cilium biogenesis; positive regulation of cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; negative regulation of epithelial cell proliferation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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