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NOTCH3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242006

MBS242006 is an antibody targeting NOTCH3. Also known as Goat Polyclonal to Human NOTCH3. Reactive in Bat, Human, Monkey, Mouse, Rat. Host: Goat. More details

$680 / 0.05 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NOTCH3, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human NOTCH3
Product Gene Name
anti-NOTCH3 antibody
Product Synonym Names
Anti-NOTCH3 Antibody (C-Terminus) IHC-plus; NOTCH3; CASIL; CADASIL; Notch 3; Notch (Drosophila) homolog 3; Notch homolog 3; Notch homolog 3 (Drosophila); Human NOTCH3
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bat, Human, Monkey, Mouse, Rat
Form/Format
TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml (lot specific)
Specificity
Human NOTCH3.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
OMIM
gene 615293
3D Structure
ModBase 3D Structure for Q9UM47
Target
NOTCH3
Immunogen Species
NOTCH3 antibody was raised against Human
Immunogen
NOTCH3 antibody was raised against peptide with sequence C-QLGPQPEVTPKRQ, from the C-Terminus of protein sequence according to NP_000426.2
Family/Subfamily
Fibronectin / Not assigned-Fibronectin
Epitope
aa2306-2318
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-NOTCH3 antibody

Family: Fibronectin
Applications Tested/Suitable for anti-NOTCH3 antibody
Immunohistochemistry (IHC - Paraffin), Peptide Enzyme-Linked Immunosorbent Assay
Application Notes for anti-NOTCH3 antibody
IHC - Paraffin (5 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:16000)

(Optimal dilution to be determined by the researcher)

Immunohistochemistry (IHC) of anti-NOTCH3 antibody

Anti-NOTCH3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-NOTCH3 Polyclonal Antibody – Cat# MBS242006

Other Notes

Small volumes of anti-NOTCH3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NOTCH3. It may not necessarily be applicable to this product.

NCBI GI #
134244285
NCBI GeneID
4854
NCBI Accession #
NP_000426.2
UniProt Primary Accession #
Q9UM47
UniProt Secondary Accession #
Q9UEB3; Q9UPL3; Q9Y6L8
UniProt Related Accession #
Q9UM47
GenBank Nucleotide #
NM_000435.2
NCBI Official Full Name
neurogenic locus notch homolog protein 3
NCBI Official Synonym Full Names
notch 3
NCBI Official Symbol
NOTCH3
NCBI Official Synonym Symbols
IMF2; CASIL; CADASIL
UniProt Protein Name
Neurogenic locus notch homolog protein 3
Protein Family
Neurogenic locus notch homolog protein
UniProt Gene Name
NOTCH3
UniProt Synonym Gene Names
Notch 3
UniProt Entry Name
NOTC3_HUMAN
NCBI Protein Information
neurogenic locus notch homolog protein 3; Notch homolog 3

NCBI Summary for NOTCH3

This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008]
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UniProt Comments for NOTCH3

NOTCH3: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH3. Interacts with PSMA1. Interacts with HIF1AN. Ubiquitously expressed in fetal and adult tissues. Belongs to the NOTCH family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2-p13.1

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cytoplasm; extracellular region; integral to membrane; plasma membrane; cytosol; receptor complex; actin cytoskeleton

Molecular Function: protein binding; enzyme binding; calcium ion binding

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; forebrain development; neuron fate commitment; Notch receptor processing; gene expression

Disease: Cerebral Arteriopathy, Autosomal Dominant, With Subcortical Infarcts And Leukoencephalopathy; Lateral Meningocele Syndrome; Myofibromatosis, Infantile, 2
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Research Articles on NOTCH3

1. Notch3 activation activates nitric oxide/soluble guanylyl cyclase signalling in immortalized ovarian surface epithelial cells and ovarian cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NOTCH3 antibody

 Reference Product  PubMed Publications
 NOTCH1 antibody  >247 publications with NOTCH3 and NOTCH1
 JAG1 antibody  >34 publications with NOTCH3 and JAG1
 JAG2 antibody  >27 publications with NOTCH3 and JAG2
 RBPJ antibody  >17 publications with NOTCH3 and RBPJ
 DLL1 antibody  >16 publications with NOTCH3 and DLL1
 MAML1 antibody  >6 publications with NOTCH3 and MAML1
 MAML2 antibody  >1 publications with NOTCH3 and MAML2
 MAML3 antibody  >1 publications with NOTCH3 and MAML3

Pathways associated with anti-NOTCH3 antibody

 Products by Pathway  Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram

Diseases associated with anti-NOTCH3 antibody

 Disease Name  Pubmed Publications
 CADASIL Antibodies  >454 publications with NOTCH3 and CADASIL
 Cardiovascular Diseases Antibodies  >435 publications with NOTCH3 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >51 publications with NOTCH3 and Disease Models, Animal
 Breast Neoplasms Antibodies  >33 publications with NOTCH3 and Breast Neoplasms
 Fibrosis Antibodies  >25 publications with NOTCH3 and Fibrosis
 Cell Transformation, Neoplastic Antibodies  >24 publications with NOTCH3 and Cell Transformation, Neoplastic
 Cognition Disorders Antibodies  >22 publications with NOTCH3 and Cognition Disorders
 Lung Neoplasms Antibodies  >22 publications with NOTCH3 and Lung Neoplasms
 Liver Diseases Antibodies  >21 publications with NOTCH3 and Liver Diseases
 Inflammation Antibodies  >20 publications with NOTCH3 and Inflammation

Organs/Tissues associated with anti-NOTCH3 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >302 publications with NOTCH3 and Vascular
 Brain Antibodies  >259 publications with NOTCH3 and Brain
 Skin Antibodies  >82 publications with NOTCH3 and Skin
 Lung Antibodies  >52 publications with NOTCH3 and Lung
 Nerve Antibodies  >48 publications with NOTCH3 and Nerve
 Embryonic Tissue Antibodies  >39 publications with NOTCH3 and Embryonic Tissue
 Ganglia Antibodies  >34 publications with NOTCH3 and Ganglia
 Bone Antibodies  >34 publications with NOTCH3 and Bone
 Liver Antibodies  >31 publications with NOTCH3 and Liver
 Kidney Antibodies  >23 publications with NOTCH3 and Kidney
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