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TREM2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242024

MBS242024 is an antibody targeting TREM2. Also known as Goat Polyclonal to Human TREM2 / TREM-2. Reactive in Human. Host: TREM2/TREM-2 antibody was produced in Goat. More details

$680 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TREM2 / TREM-2, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human TREM2 / TREM-2
Product Gene Name
anti-TREM2 antibody
Product Synonym Gene Name
TREM-2
Product Synonym Names
Anti-TREM2 / TREM-2 Antibody (C-Terminus) IHC-plus; TREM2; Trem2b; TREM-2; Trem2a; Trem2c; Human TREM2; TREM-2
Clonality
Polyclonal
Host
TREM2/TREM-2 antibody was produced in Goat
Species Reactivity
Human
Form/Format
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5mg/ml (lot specific)
Specificity
Human TREM2
Preparation and Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
OMIM
phenotype 605086
3D Structure
ModBase 3D Structure for Q9NZC2
Target
TREM2/TREM-2
Immunogen Species
TREM2/TREM-2 antibody was raised against Human
Immunogen
TREM2/TREM-2 antibody was raised against peptide with sequence C-HGQKPGTHPPSELD, from the C-Terminus of protein sequence according to NP_061838.1.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-TREM2 antibody
Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Peptide Enzyme-Linked Immunosorbent Assay
Application Notes for anti-TREM2 antibody
IHC - Paraffin (2.5 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Usage:
Immunohistochemistry: MBS242024 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242024 was determined to be 2.5 ug/ml.
(Optimal dilution to be determined by the researcher.)

Immunohistochemistry (IHC) of anti-TREM2 antibody

Anti-TREM2 antibody IHC of human spleen, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-TREM2 Polyclonal Antibody – Cat# MBS242024

Other Notes

Small volumes of anti-TREM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TREM2. It may not necessarily be applicable to this product.

NCBI GI #
9507203
NCBI GeneID
54209
NCBI Accession #
NP_061838.1
UniProt Primary Accession #
Q9NZC2
UniProt Secondary Accession #
Q8N5H8; Q8WYN6
UniProt Related Accession #
Q9NZC2
GenBank Nucleotide #
NM_018965.3
NCBI Official Full Name
triggering receptor expressed on myeloid cells 2 isoform 1
NCBI Official Synonym Full Names
triggering receptor expressed on myeloid cells 2
NCBI Official Symbol
TREM2
NCBI Official Synonym Symbols
TREM-2; Trem2a; Trem2b; Trem2c
UniProt Protein Name
Triggering receptor expressed on myeloid cells 2
UniProt Synonym Protein Names
Triggering receptor expressed on monocytes 2
Protein Family
Triggering receptor expressed on myeloid cells
UniProt Gene Name
TREM2
UniProt Synonym Gene Names
TREM-2
UniProt Entry Name
TREM2_HUMAN
NCBI Protein Information
triggering receptor expressed on myeloid cells 2; triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2a

NCBI Summary for TREM2

This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
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UniProt Comments for TREM2

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: intracellular membrane-bound organelle; extracellular region; plasma membrane; integral to membrane

Molecular Function: peptidoglycan binding; lipopolysaccharide binding; receptor activity

Biological Process: axon guidance; positive regulation of peptidyl-tyrosine phosphorylation; detection of lipopolysaccharide; detection of peptidoglycan; innate immune response; positive regulation of calcium-mediated signaling; humoral immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II

Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy
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Research Articles on TREM2

1. results serve to confirm the association between R47H and risk of AD, the observed effect on risk was substantially smaller than that previously reported.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TREM2 antibody

 Reference Product  PubMed Publications
 TYROBP antibody  >48 publications with TREM2 and TYROBP
 SYK antibody  >8 publications with TREM2 and SYK
 PLXNA1 antibody  >2 publications with TREM2 and PLXNA1
 VAV3 antibody  >1 publications with TREM2 and VAV3
 SEMA6D antibody  >1 publications with TREM2 and SEMA6D

Pathways associated with anti-TREM2 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Other Semaphorin Interactions Pathway antibodies  Other Semaphorin Interactions Pathway Diagram
 Semaphorin Interactions Pathway antibodies  Semaphorin Interactions Pathway Diagram

Diseases associated with anti-TREM2 antibody

 Disease Name  Pubmed Publications
 Dementia Antibodies  >87 publications with TREM2 and Dementia
 Nervous System Diseases Antibodies  >78 publications with TREM2 and Nervous System Diseases
 Alzheimer Disease Antibodies  >59 publications with TREM2 and Alzheimer Disease
 Inflammation Antibodies  >53 publications with TREM2 and Inflammation
 Polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy Antibodies  >23 publications with TREM2 and Polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy
 Disease Models, Animal Antibodies  >14 publications with TREM2 and Disease Models, Animal
 Necrosis Antibodies  >12 publications with TREM2 and Necrosis
 Skin Diseases Antibodies  >10 publications with TREM2 and Skin Diseases
 Lung Diseases Antibodies  >8 publications with TREM2 and Lung Diseases
 Memory Disorders Antibodies  >4 publications with TREM2 and Memory Disorders

Organs/Tissues associated with anti-TREM2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >68 publications with TREM2 and Brain
 Lung Antibodies  >12 publications with TREM2 and Lung
 Eye Antibodies  >2 publications with TREM2 and Eye
 Ovary Antibodies  >2 publications with TREM2 and Ovary
 Kidney Antibodies  >1 publications with TREM2 and Kidney
 Adipose Tissue Antibodies  >1 publications with TREM2 and Adipose Tissue
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