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AIF1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242026

Allograft inflammatory Factor 1 (AIF1) is a calcium binding peptide produced by activated monocytes and microglial cells. AIF1 expression is associated with chronic inflammatory processes and is also transiently expressed in response to vascular trauma. More details

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Product Overview

Online Datasheet in PDF
Product Name
AIF1 / IBA1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human AIF1 / IBA1
Product Gene Name
anti-AIF1 antibody
Product Synonym Gene Name
IBA1
Product Synonym Names
Anti-AIF1 / IBA1 Antibody (Internal) IHC-plus; AIF1; AIF-1; Em:AF129756.17; G1; IRT-1; Protein G1; IBA1; IRT1; Human AIF1; IBA1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Dog, Gorilla, Horse, Human, Pig
Predicted Reactivity: Monkey, Mouse, Rat, Bat, Bovine, Goat (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human AIF1 / IBA1. This antibody is expected to recognise isoform 3 (NP_001614.3) only.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
14,617 Da
OMIM
601833
3D Structure
ModBase 3D Structure for P55008
Target Species
Human
Immunogen Description
Synthetic peptide C-NKQFLDDPKYSSDED from an internal region of human AIF1 / IBA1 (NP_001614.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Panda, Dog, Horse, Pig (100%); Marmoset, Mouse, Rat, Goat, Bovine, Bat (93%).
Immunogen Type
Synthetic peptide
Immunogen
AIF1 / IBA1 antibody was raised against synthetic peptide C-NKQFLDDPKYSSDED from an internal region of human AIF1 / IBA1 (NP_001614.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Panda, Dog, Horse, Pig (100%); Marmoset, Mouse, Rat, Goat, Bovine, Bat (93%); Rabbit (87%); Platypus (80%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-AIF1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-AIF1 antibody
ELISA (1:32000), IHC-P (2.5 ug/ml), WB (1:32000, 1 - 3 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-AIF1 antibody

Anti-AIF1 / IBA1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-AIF1 Polyclonal Antibody – Cat# MBS242026

Western Blot (WB) of anti-AIF1 antibody

Antibody (1 ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-AIF1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242026

Other Notes

Small volumes of anti-AIF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AIF1 purchase

MBS242026 is an antibody targeting AIF1. Also known as Goat Polyclonal to Human AIF1 / IBA1. Reactive in Gibbon, Dog, Gorilla, Horse, Human, Pig
Predicted Reactivity: Monkey, Mouse, Rat, Bat, Bovine, Goat (at least 90% immunogen sequence identity). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for AIF1. It may not necessarily be applicable to this product.

NCBI GI #
4758612
NCBI GeneID
199
NCBI Accession #
NP_004838.1
UniProt Primary Accession #
P55008
UniProt Secondary Accession #
O43904; Q9UIV4; Q9UKS9; A8K406
UniProt Related Accession #
P55008
GenBank Nucleotide #
NM_004847.3
NCBI Official Full Name
allograft inflammatory factor 1 isoform 2
NCBI Official Synonym Full Names
allograft inflammatory factor 1
NCBI Official Symbol
AIF1
NCBI Official Synonym Symbols
IBA1; IRT1; AIF-1; IRT-1
UniProt Protein Name
Allograft inflammatory factor 1
UniProt Synonym Protein Names
Ionized calcium-binding adapter molecule 1; Protein G1
Protein Family
Allograft inflammatory factor
UniProt Gene Name
AIF1
UniProt Synonym Gene Names
G1; IBA1; AIF-1
UniProt Entry Name
AIF1_HUMAN
NCBI Protein Information
allograft inflammatory factor 1; protein G1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; allograft inflammatory factor-1 splice variant Hara-1

NCBI Summary for AIF1

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
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UniProt Comments for AIF1

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: perinuclear region of cytoplasm; lamellipodium; cytoplasm; actin filament; perikaryon; nucleus; cytosol; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; Rac protein signal transduction; microglial cell activation; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; response to axon injury; response to electrical stimulus; inflammatory response; phagocytosis, engulfment; negative regulation of apoptosis
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Research Articles on AIF1

1. AIF-1 functions as a tumor suppressor possibly by regulating beta-catenin in gastric cancer. the level of AIF-1 expression may serve as a protective prognostic indicator for gastric cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AIF1 antibody

 Reference Product  PubMed Publications
 GFAP antibody  >97 publications with AIF1 and GFAP
 RAC1 antibody  >4 publications with AIF1 and RAC1
 TYROBP antibody  >1 publications with AIF1 and TYROBP

Pathways associated with anti-AIF1 antibody

 Products by Pathway  Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram

Diseases associated with anti-AIF1 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >277 publications with AIF1 and Disease Models, Animal
 Inflammation Antibodies  >155 publications with AIF1 and Inflammation
 Death Antibodies  >115 publications with AIF1 and Death
 Cardiovascular Diseases Antibodies  >99 publications with AIF1 and Cardiovascular Diseases
 Necrosis Antibodies  >90 publications with AIF1 and Necrosis
 Nerve Degeneration Antibodies  >43 publications with AIF1 and Nerve Degeneration
 Cognition Disorders Antibodies  >16 publications with AIF1 and Cognition Disorders
 Neurotoxicity Syndromes Antibodies  >16 publications with AIF1 and Neurotoxicity Syndromes
 Edema Antibodies  >13 publications with AIF1 and Edema
 Parkinson Disease Antibodies  >12 publications with AIF1 and Parkinson Disease

Organs/Tissues associated with anti-AIF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >412 publications with AIF1 and Brain
 Blood Antibodies  >143 publications with AIF1 and Blood
 Vascular Antibodies  >62 publications with AIF1 and Vascular
 Muscle Antibodies  >48 publications with AIF1 and Muscle
 Bone Marrow Antibodies  >39 publications with AIF1 and Bone Marrow
 Eye Antibodies  >29 publications with AIF1 and Eye
 Embryonic Tissue Antibodies  >19 publications with AIF1 and Embryonic Tissue
 Heart Antibodies  >18 publications with AIF1 and Heart
 Spleen Antibodies  >14 publications with AIF1 and Spleen
 Skin Antibodies  >14 publications with AIF1 and Skin
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