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GOT2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242053

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles.… More details

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Product Overview

Product Name
GOT2, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human GOT2
Product Gene Name
anti-GOT2 antibody
Product Synonym Names
Anti-GOT2 Antibody (aa295-306) IHC-plus; GOT2; FABP-1; MitAAT; Transaminase A; FABPpm; Fatty acid-binding protein; KAT4; KATIV; Kynurenine aminotransferase IV; MAspAT; Human GOT2
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gorilla, Hamster, Horse, Human, Monkey, Orangutan
Predicted Reactivity: Mouse, Rat, Bovine, Pig, Zebrafish (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human GOT2.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
43,030 Da
OMIM
138150
3D Structure
ModBase 3D Structure for P00505
Target Species
Human
Immunogen Description
Synthetic peptide CKDADEAKRVES from an internal region of human GOT2 (NP_002071.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Hamster, Panda, Horse, Opossum (100%).
Immunogen Type
Synthetic peptide
Immunogen
GOT2 antibody was raised against synthetic peptide CKDADEAKRVES from an internal region of human GOT2 (NP_002071.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Hamster, Panda, Horse, Opossum (100%); Mouse, Rat, Bovine, Pig, Catfish, Salmon, Zebrafish (92%); Elephant, Turkey, Chicken, Platypus, Xenopus, Pufferfish, Poplar, Castor bean (83%).
Antigen Modification
aa295-306
Applications Tested/Suitable for anti-GOT2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-GOT2 antibody
ELISA (1:32000), IHC-P (5 ug/ml), WB (0.03 - 0.1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-GOT2 antibody

Anti-GOT2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-GOT2 Polyclonal Antibody – Cat# MBS242053

Western Blot (WB) of anti-GOT2 antibody

Antibody (0.03 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-GOT2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242053

Other Notes

Small volumes of anti-GOT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOT2 purchase

MBS242053 is an antibody targeting GOT2. Also known as Goat Polyclonal to Human GOT2. Reactive in Gorilla, Hamster, Horse, Human, Monkey, Orangutan
Predicted Reactivity: Mouse, Rat, Bovine, Pig, Zebrafish (at least 90% immunogen sequence identity). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for GOT2. It may not necessarily be applicable to this product.

NCBI GI #
73486658
NCBI GeneID
2806
NCBI Accession #
NP_002071.2
UniProt Primary Accession #
P00505
UniProt Secondary Accession #
Q53FL3; Q9BWA3; B4DJA6; E7ERW2
UniProt Related Accession #
P00505
GenBank Nucleotide #
NM_002080.3
NCBI Official Full Name
aspartate aminotransferase, mitochondrial isoform 1
NCBI Official Synonym Full Names
glutamic-oxaloacetic transaminase 2, mitochondrial
NCBI Official Symbol
GOT2
NCBI Official Synonym Symbols
KAT4; KATIV; mitAAT
UniProt Protein Name
Aspartate aminotransferase, mitochondrial
UniProt Synonym Protein Names
Fatty acid-binding protein; FABP-1; Glutamate oxaloacetate transaminase 2; Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; Plasma membrane-associated fatty acid-binding protein; FABPpm; Transaminase A
UniProt Gene Name
GOT2
UniProt Synonym Gene Names
mAspAT; FABP-1; FABPpm
UniProt Entry Name
AATM_HUMAN
NCBI Protein Information
aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; mAspAT; transaminase A; aspartate transaminase 2; fatty acid-binding protein; aspartate aminotransferase 2; kynurenine aminotransferase 4; kynurenine aminotransferase IV; glutamate oxaloacetate transaminase 2; kynurenine--oxoglutarate transaminase 4; kynurenine--oxoglutarate transaminase IV; plasma membrane-associated fatty acid-binding protein; glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)

NCBI Summary for GOT2

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]
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UniProt Comments for GOT2

GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: EC 2.6.1.7; Transferase; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; EC 2.6.1.1; Mitochondrial; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane

Molecular Function: kynurenine-oxoglutarate transaminase activity; aspartate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; aspartate biosynthetic process; glucose metabolic process; pathogenesis; 2-oxoglutarate metabolic process; gluconeogenesis; response to ethanol; 4-hydroxyproline catabolic process; carbohydrate metabolic process; aspartate metabolic process; aspartate catabolic process; glutamate catabolic process to 2-oxoglutarate; amino acid biosynthetic process; fatty acid transport
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Research Articles on GOT2

1. The lipid binding process in L-FABP is associated with backbone dynamics.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GOT2 antibody

 Reference Product  PubMed Publications
 GOT1 antibody  >7 publications with GOT2 and GOT1
 MDH2 antibody  >4 publications with GOT2 and MDH2
 MDH1 antibody  >3 publications with GOT2 and MDH1
 IDH2 antibody  >2 publications with GOT2 and IDH2

Pathways associated with anti-GOT2 antibody

 Products by Pathway  Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 4-hydroxyproline Degradation Pathway antibodies  4-hydroxyproline Degradation Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram

Diseases associated with anti-GOT2 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >3 publications with GOT2 and Disease Models, Animal
 Mental Disorders Antibodies  >2 publications with GOT2 and Mental Disorders
 Cardiovascular Diseases Antibodies  >2 publications with GOT2 and Cardiovascular Diseases
 Cardiomyopathies Antibodies  >1 publications with GOT2 and Cardiomyopathies
 Liver Diseases Antibodies  >1 publications with GOT2 and Liver Diseases
 Fatty Liver Antibodies  >1 publications with GOT2 and Fatty Liver
 Hypertension Antibodies  >1 publications with GOT2 and Hypertension
 Nervous System Diseases Antibodies  >1 publications with GOT2 and Nervous System Diseases
 Heart Diseases Antibodies  >1 publications with GOT2 and Heart Diseases

Organs/Tissues associated with anti-GOT2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with GOT2 and Blood
 Thyroid Antibodies  >3 publications with GOT2 and Thyroid
 Muscle Antibodies  >3 publications with GOT2 and Muscle
 Liver Antibodies  >3 publications with GOT2 and Liver
 Brain Antibodies  >3 publications with GOT2 and Brain
 Vascular Antibodies  >2 publications with GOT2 and Vascular
 Heart Antibodies  >2 publications with GOT2 and Heart
 Adrenal Gland Antibodies  >1 publications with GOT2 and Adrenal Gland
 Intestine Antibodies  >1 publications with GOT2 and Intestine
 Kidney Antibodies  >1 publications with GOT2 and Kidney
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