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ALDH1A1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242075

MBS242075 is an antibody targeting ALDH1A1. Also known as Goat Polyclonal to Human ALDH1A1 / ALDH1. Reactive in Gibbon, Bovine, Gorilla, Human, Orangutan, SheepPredicted Reactivity: Monkey, Bat, Horse (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
ALDH1A1 / ALDH1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human ALDH1A1 / ALDH1
Product Gene Name
Product Synonym Gene Name
ALDH1
Product Synonym Names
Anti-ALDH1A1 / ALDH1 Antibody (Internal) IHC-plus; ALDH1A1; Acetaldehyde dehydrogenase 1; ALDH class 1; ALDC; Aldehyde dehydrogenase 1; ALDH-E1; ALDH1; ALDH11; PUMB1; RALDH 1; RALDH1; ALHDII; Retinal dehydrogenase 1; Retinaldehyde dehydrogenase 1; Human ALDH1A1; ALDH1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Bovine, Gorilla, Human, Orangutan, Sheep
Predicted Reactivity: Monkey, Bat, Horse (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human ALDH1A1.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
54,862 Da
OMIM
100640
3D Structure
ModBase 3D Structure for P00352
Target Species
Human
Immunogen Description
Synthetic peptide C-RELGEYGFHEYTE from an internal region of human ALDH1A1 (NP_000680.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Sheep, Bovine (100%); Marmoset, Bat, Horse, Opossum, Lizard (92%).
Immunogen Type
Synthetic peptide
Immunogen
ALDH1A1 / ALDH1 antibody was raised against synthetic peptide C-RELGEYGFHEYTE from an internal region of human ALDH1A1 (NP_000680.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Sheep, Bovine (100%); Marmoset, Bat, Horse, Opossum, Lizard (92%); Dog, Chicken, Platypus, Xenopus (85%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-ALDH1A1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ALDH1A1 antibody
ELISA (1:32000), IHC-P (2.5 ug/ml), WB (1:32000, 1 - 3 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-ALDH1A1 antibody

Anti-ALDH1A1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-ALDH1A1 Polyclonal Antibody – Cat# MBS242075

Western Blot (WB) of anti-ALDH1A1 antibody

Antibody (1 ug/ml) staining of Human Liver Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ALDH1A1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242075

Other Notes

Small volumes of anti-ALDH1A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ALDH1A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
retinal dehydrogenase 1
NCBI Official Synonym Full Names
aldehyde dehydrogenase 1 family, member A1
NCBI Official Symbol
ALDH1A1
NCBI Official Synonym Symbols
ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
UniProt Protein Name
Retinal dehydrogenase 1
UniProt Synonym Protein Names
ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic
Protein Family
UniProt Gene Name
ALDH1A1
UniProt Synonym Gene Names
ALDC; ALDH1; PUMB1; RALDH 1; RalDH1
UniProt Entry Name
AL1A1_HUMAN
NCBI Protein Information
retinal dehydrogenase 1; ALHDII; RALDH 1; ALDH class 1; acetaldehyde dehydrogenase 1; epididymis luminal protein 9; epididymis luminal protein 12; retinaldehyde dehydrogenase 1; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase, liver cytosolic; epididymis secretory sperm binding protein Li 53e

NCBI Summary for ALDH1A1

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]
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UniProt Comments for ALDH1A1

ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.

Protein type: EC 1.2.1.36; Oxidoreductase; GAPs, Ras; Apoptosis; GAPs; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: cytoplasm; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; 3-chloroallyl aldehyde dehydrogenase activity; benzaldehyde dehydrogenase (NAD+) activity; retinal dehydrogenase activity

Biological Process: retinol metabolic process; xenobiotic metabolic process; aldehyde metabolic process; ethanol oxidation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ALDH1A1 antibody

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 Defective CYP21A2 Causes Adrenal Hyperplasia 3 (AH3) Pathway antibodies  Defective CYP21A2 Causes Adrenal Hyperplasia 3 (AH3) Pathway Diagram
 Defective CYP24A1 Causes Hypercalcemia, Infantile (HCAI) Pathway antibodies  Defective CYP24A1 Causes Hypercalcemia, Infantile (HCAI) Pathway Diagram
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