OGT Antibody – Goat Polyclonal

This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

OGT: Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta- linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. Heterotrimer; consists of one 78 kDa subunit and two 110 kDa subunits dimerized via TPR repeats 6 and 7. Interacts (via TPR repeats 6 and 7) with ATXN10. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, HCFC1, PPP1CC and ACTB. Component of a THAP1/THAP3-HCFC1-OGT complex. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts directly with HCFC1; the interaction O- glycosylates HCFC1, regulates its proteolytic processing and transcriptional activity and, in turn, stabilizes OGT in the nucleus. Interacts (via TPRs 1-6) with SIN3A; the interaction mediates transcriptional repression in parallel with histone deacetylase. Induction of the nucleocytoplasmic OGT (ncOGT) isoform in the liver on glucose deprivation is mediated by the decreased hexosamine biosynthesis pathway (HBP) flux. Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. Subject to product inhibition by UDP. Belongs to the O-GlcNAc transferase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - O-glycan biosynthesis; Transferase; EC 2.4.1.255

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; plasma membrane; microtubule organizing center; cytosol; nucleus; histone acetyltransferase complex

Molecular Function: acetylglucosaminyltransferase activity; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; protein N-acetylglucosaminyltransferase activity; enzyme activator activity; histone acetyltransferase activity (H4-K16 specific)

Biological Process: positive regulation of catalytic activity; protein amino acid O-linked glycosylation; establishment and/or maintenance of chromatin architecture; phosphoinositide-mediated signaling; regulation of insulin receptor signaling pathway; apoptosis; positive regulation of proteolysis; positive regulation of granulocyte differentiation; positive regulation of histone H3-K4 methylation; signal transduction; response to insulin stimulus; regulation of Rac protein signal transduction; regulation of glycolysis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein ubiquitination; circadian regulation of gene expression; response to nutrient