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IL33 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242172

Interleukin-33 (IL33) is a cytokine belonging to the IL-1 superfamily. IL33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IL33, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human IL33
Product Gene Name
anti-IL33 antibody
Product Synonym Names
IL33; C9orf26; DVS27; DVS27-related protein; IL-1F11; IL-33; Interleukin-33; NFEHEV; NF-HEV; Interleukin-1 family member 11; NFHEV; IL1F11; Interleukin 33; RP11-575C20.2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
a 19 amino acid peptide from near the center of human IL-33
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
16,250 Da
OMIM
608678
3D Structure
ModBase 3D Structure for O95760
Immunogen
IL-33 antibody was raised against synthetic peptide from human IL33 / IL-33
Antigen Type
Synthetic peptide
Epitope
Internal
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-IL33 antibody

Family: Interleukin
Applications Tested/Suitable for anti-IL33 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-IL33 antibody
ELISA, IHC-P (5 ug/ml), Western Blot (1 ug/ml)
Other dilution to be determined by researcher.

Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antbibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

Immunohistochemistry (IHC) of anti-IL33 antibody

Anti-IL-33 antibody IHC of human uterus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-IL33 Polyclonal Antibody – Cat# MBS242172

Immunofluorescence (IF) of anti-IL33 antibody

Immunofluorescence of IL-33 in Human Lymph Node cells with IL-33 antibody at 20 ug/ml.
Rabbit Anti-IL33 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS242172

Western Blot (WB) of anti-IL33 antibody

Western blot of IL-33 in human lymph node tissue lysate with IL-33 antibody at (A) 1 and (B) 2 ug/ml.
Rabbit Anti-IL33 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242172

Other Notes

Small volumes of anti-IL33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL33 purchase

MBS242172 is an antibody targeting IL33. Also known as Rabbit Polyclonal (IgG) to Human IL33. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL33. It may not necessarily be applicable to this product.

NCBI GI #
15559209
NCBI GeneID
90865
NCBI Accession #
NP_254274.1
UniProt Primary Accession #
O95760
UniProt Secondary Accession #
Q2YEJ5; B2R8L1; B4DJ35; B4E1Q9; D3DRI5; E7EAX4
UniProt Related Accession #
O95760
GenBank Nucleotide #
NM_033439.3
NCBI Official Full Name
interleukin-33 isoform 1
NCBI Official Synonym Full Names
interleukin 33
NCBI Official Symbol
IL33
NCBI Official Synonym Symbols
DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
UniProt Protein Name
Interleukin-33
UniProt Synonym Protein Names
Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV
Protein Family
Interleukin
UniProt Gene Name
IL33
UniProt Synonym Gene Names
C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV
UniProt Entry Name
IL33_HUMAN
NCBI Protein Information
interleukin-33; IL-33; IL-1F11; DVS27-related protein; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules

NCBI Summary for IL33

IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]
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UniProt Comments for IL33

IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular space; transport vesicle; chromosome; nucleus

Molecular Function: protein binding; cytokine activity

Biological Process: negative regulation of T-helper 1 type immune response; T-helper 2 type immune response; positive regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; negative regulation of interferon-gamma production; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; positive regulation of interleukin-5 production; positive regulation of inflammatory response; positive regulation of macrophage activation
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Research Articles on IL33

1. Data indicate an association exists between interleukin-33 (IL-33) serum levels and restenosis in coronary artery disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL33 antibody

 Reference Product  PubMed Publications
 IL1RL1 antibody  >154 publications with IL33 and IL1RL1
 TSLP antibody  >19 publications with IL33 and TSLP
 CASP1 antibody  >17 publications with IL33 and CASP1
 IL18 antibody  >16 publications with IL33 and IL18
 IL25 antibody  >14 publications with IL33 and IL25
 IL18R1 antibody  >7 publications with IL33 and IL18R1
 VEGFA antibody  >3 publications with IL33 and VEGFA
 IL1B antibody  >2 publications with IL33 and IL1B
 CSF2 antibody  >1 publications with IL33 and CSF2

Pathways associated with anti-IL33 antibody

 Products by Pathway  Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram

Diseases associated with anti-IL33 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >184 publications with IL33 and Inflammation
 Asthma Antibodies  >85 publications with IL33 and Asthma
 Hypersensitivity, Immediate Antibodies  >82 publications with IL33 and Hypersensitivity, Immediate
 Lung Diseases Antibodies  >80 publications with IL33 and Lung Diseases
 Necrosis Antibodies  >55 publications with IL33 and Necrosis
 Cardiovascular Diseases Antibodies  >48 publications with IL33 and Cardiovascular Diseases
 Heart Diseases Antibodies  >29 publications with IL33 and Heart Diseases
 Dermatitis, Atopic Antibodies  >18 publications with IL33 and Dermatitis, Atopic
 Disease Models, Animal Antibodies  >18 publications with IL33 and Disease Models, Animal
 Nervous System Diseases Antibodies  >12 publications with IL33 and Nervous System Diseases

Organs/Tissues associated with anti-IL33 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >52 publications with IL33 and Lung
 Heart Antibodies  >42 publications with IL33 and Heart
 Skin Antibodies  >41 publications with IL33 and Skin
 Vascular Antibodies  >32 publications with IL33 and Vascular
 Muscle Antibodies  >19 publications with IL33 and Muscle
 Intestine Antibodies  >17 publications with IL33 and Intestine
 Liver Antibodies  >16 publications with IL33 and Liver
 Brain Antibodies  >11 publications with IL33 and Brain
 Eye Antibodies  >9 publications with IL33 and Eye
 Connective Tissue Antibodies  >7 publications with IL33 and Connective Tissue
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