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ALS2CR1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242199

MBS242199 is an antibody targeting ALS2CR1. Also known as Goat Polyclonal to Human ALS2CR1 / NIF3L1. Reactive in Gibbon, Gorilla, Human, MonkeyPredicted Reactivity: Mouse, Rat, Bat, Dog, Hamster, Horse, Chicken (at least 90% immunogen sequence identity).… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
ALS2CR1 / NIF3L1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human ALS2CR1 / NIF3L1
Product Gene Name
Product Synonym Gene Name
NIF3L1
Product Synonym Names
Anti-ALS2CR1 / NIF3L1 Antibody (C-Terminus) IHC-plus; NIF3L1; CALS-7; MDS015; NIF3-like protein 1; ALS2CR1; Human ALS2CR1; NIF3L1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Gorilla, Human, Monkey
Predicted Reactivity: Mouse, Rat, Bat, Dog, Hamster, Horse, Chicken (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human NIF3L1. This antibody is expected to recognise both reported isoforms. Variants (NP_001135827.1; NP_068596.2) encode the same isoform.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
32,164 Da
OMIM
605778
3D Structure
ModBase 3D Structure for Q9GZT8
Target Species
Human
Immunogen Description
Synthetic peptide C-ILSETDRDPLQVV from the C-terminus of human NIF3L1 (NP_001129511.1; NP_068596.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Opossum (100%).
Immunogen Type
Synthetic peptide
Immunogen
ALS2CR1 / NIF3L1 antibody was raised against synthetic peptide C-ILSETDRDPLQVV from the C-terminus of human NIF3L1 (NP_001129511.1; NP_068596.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Opossum (100%); Mouse, Rat, Hamster, Panda, Bat, Dog, Horse, Turkey, Chicken (92%); Goat, Elephant, Bovine (85%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-ALS2CR1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ALS2CR1 antibody
ELISA (1:32000), IHC-P (2.5 ug/ml), WB (0.2 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-ALS2CR1 antibody

Anti-NIF3L1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-ALS2CR1 Polyclonal Antibody – Cat# MBS242199

Western Blot (WB) of anti-ALS2CR1 antibody

Antibody staining (1 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Goat Anti-ALS2CR1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242199

Other Notes

Small volumes of anti-ALS2CR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ALS2CR1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
putative GTP cyclohydrolase 1 type 2 NIF3L1 isoform 2
NCBI Official Synonym Full Names
NIF3 NGG1 interacting factor 3-like 1 (S. cerevisiae)
NCBI Official Symbol
NIF3L1
NCBI Official Synonym Symbols
CALS-7; MDS015; ALS2CR1
UniProt Protein Name
Putative GTP cyclohydrolase 1 type 2 NIF3L1
UniProt Synonym Protein Names
Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 1 protein; GTP cyclohydrolase I; NIF3-like protein 1
UniProt Gene Name
NIF3L1
UniProt Synonym Gene Names
ALS2CR1
UniProt Entry Name
GTPC1_HUMAN
NCBI Protein Information
putative GTP cyclohydrolase 1 type 2 NIF3L1; NIF3-like protein 1; GTP cyclohydrolase I; NIF3 (Ngg1 interacting factor 3, S.pombe homolog)-like 1; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 1; amyotrophic lateral sclerosis 2 chromosomal region candidate gene 1 protein

UniProt Comments for ALS2CR1

NIF3L1: Belongs to the UPF0135 (NIF3) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.4.16; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: mitochondrion; cytoplasm

Molecular Function: GTP cyclohydrolase I activity; protein binding; GTP binding; metal ion binding; transcription factor binding

Biological Process: positive regulation of transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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