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AOC3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242214

MBS242214 is an antibody targeting AOC3. Also known as Goat Polyclonal to Human AOC3 / VAP-1. Reactive in Human, HamsterPredicted Reactivity: Monkey, Mouse, Rat, Rabbit. Host: AOC3/VAP-1 antibody was produced in Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AOC3 / VAP-1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human AOC3 / VAP-1
Product Gene Name
anti-AOC3 antibody
Product Synonym Gene Name
VAP-1
Product Synonym Names
Anti-AOC3 / VAP-1 Antibody (Internal) IHC-plus; AOC3; Copper amine oxidase; HPAO; Membrane primary amine oxidase; SSAO; VAP-1; VAP1; Vascular adhesion protein 1; Human AOC3
Clonality
Polyclonal
Host
AOC3/VAP-1 antibody was produced in Goat
Species Reactivity
Human, Hamster
Predicted Reactivity: Monkey, Mouse, Rat, Rabbit
Form/Format
TBS, pH 7.3, 0.02% sodium azide, 0.5% BSA
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human AOC3 / VAP-1.
Preparation and Storage
Store at -20°C. Avoid freeze-thaw cycles.
Immunogen Sequence Positions
aa712-724
OMIM
603735
3D Structure
ModBase 3D Structure for Q16853
Target
AOC3/VAP-1
Immunogen Species
AOC3/VAP-1 antibody was raised against Human
Antigen Type
Synthetic peptide
Usage
Immunohistochemistry: MBS242214 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242214 was determined to be 3.75 ug/ml.
Immunogen
AOC3 / VAP-1 antibody was raised against synthetic peptide C-DEDPSFYSADSIY from an internal region (near the C-terminus) of human AOC3 / VAP-1 (NP_003725.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Marmoset, Hamster (100%); Gibbon, Monkey, Mouse, Rat, Rabbit (92%); Elephant, Bovine (85%).
Antigen Modification
Internal
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-AOC3 antibody
Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-Paraffin), Peptide Enzyme-Linked Immunosorbent Assay
Application Notes for anti-AOC3 antibody
Immunohistochemistry-Paraffin (IHC-Paraffin) (3.75 ug/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:64000)

Optimal dilutions to be determined by the researcher).

Other Notes

Small volumes of anti-AOC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AOC3. It may not necessarily be applicable to this product.

NCBI GI #
4502119
NCBI GeneID
8639
NCBI Accession #
NP_003725.1
UniProt Primary Accession #
Q16853
UniProt Secondary Accession #
Q45F94; B2RCI5; K7ESB3; L0L8N9
UniProt Related Accession #
Q16853
GenBank Nucleotide #
NM_003734.3
NCBI Official Full Name
membrane primary amine oxidase isoform 1
NCBI Official Synonym Full Names
amine oxidase, copper containing 3
NCBI Official Symbol
AOC3
NCBI Official Synonym Symbols
HPAO; SSAO; VAP1; VAP-1
UniProt Protein Name
Membrane primary amine oxidase
UniProt Synonym Protein Names
Copper amine oxidase; HPAO; Semicarbazide-sensitive amine oxidase; SSAO; Vascular adhesion protein 1; VAP-1
Protein Family
Allene oxide cyclase
UniProt Gene Name
AOC3
UniProt Synonym Gene Names
VAP1; SSAO; VAP-1
UniProt Entry Name
AOC3_HUMAN
NCBI Protein Information
membrane primary amine oxidase; copper amine oxidase; placenta copper monamine oxidase; semicarbazide-sensitive amine oxidase; amine oxidase, copper containing 3 (vascular adhesion protein 1)

NCBI Summary for AOC3

This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]
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UniProt Comments for AOC3

AOC3: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. Belongs to the copper/topaquinone oxidase family.

Protein type: Oxidoreductase; Cell adhesion; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - phenylalanine; Other Amino Acids Metabolism - beta-alanine; EC 1.4.3.21; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: microvillus; cell surface; cytoplasm; plasma membrane; integral to membrane

Molecular Function: amine oxidase activity; protein binding; protein homodimerization activity; copper ion binding; protein heterodimerization activity; cation channel activity; calcium ion binding; quinone binding

Biological Process: response to antibiotic; amine metabolic process; cell adhesion; inflammatory response; cation transport
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Research Articles on AOC3

1. SSAO expression in endothelial cells increases oxygen-glucose-deprivation-associated cell damage. It mediates some tissue damage during the reoxygenation process by oxidizing its known enzymatic substrate, methylamine.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-AOC3 antibody

 Products by Pathway  Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Beta-Alanine Metabolism Pathway antibodies  Beta-Alanine Metabolism Pathway Diagram
 Beta-Alanine Metabolism Pathway antibodies  Beta-Alanine Metabolism Pathway Diagram
 Phenylethylamine Degradation I Pathway antibodies  Phenylethylamine Degradation I Pathway Diagram

Diseases associated with anti-AOC3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >74 publications with AOC3 and Inflammation
 Cardiovascular Diseases Antibodies  >33 publications with AOC3 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >14 publications with AOC3 and Atherosclerosis
 Necrosis Antibodies  >11 publications with AOC3 and Necrosis
 Disease Models, Animal Antibodies  >9 publications with AOC3 and Disease Models, Animal
 Liver Neoplasms Antibodies  >8 publications with AOC3 and Liver Neoplasms
 Lung Diseases Antibodies  >7 publications with AOC3 and Lung Diseases
 Hyperglycemia Antibodies  >6 publications with AOC3 and Hyperglycemia
 Liver Cirrhosis Antibodies  >5 publications with AOC3 and Liver Cirrhosis
 Hypertension Antibodies  >5 publications with AOC3 and Hypertension

Organs/Tissues associated with anti-AOC3 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >167 publications with AOC3 and Vascular
 Liver Antibodies  >31 publications with AOC3 and Liver
 Kidney Antibodies  >13 publications with AOC3 and Kidney
 Brain Antibodies  >11 publications with AOC3 and Brain
 Heart Antibodies  >11 publications with AOC3 and Heart
 Adipose Tissue Antibodies  >9 publications with AOC3 and Adipose Tissue
 Lung Antibodies  >7 publications with AOC3 and Lung
 Connective Tissue Antibodies  >6 publications with AOC3 and Connective Tissue
 Umbilical Cord Antibodies  >6 publications with AOC3 and Umbilical Cord
 Eye Antibodies  >6 publications with AOC3 and Eye
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