Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Immunofluorescence microscopy. Rabbit anti-AKT was used at a 1:80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody. Tissue: neonatal rat cardiomyocytes. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT antibody at 1:80 dilution for 1 h at RT. Secondary antibody: Texas-red conjugated rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: AKT is nuclear. Staining: Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
Western Blot. Rabbit anti-AKT was used at a 1:500 dilution to detect AKT by Western blot.
Western blot of Rabbit Anti-AKT antibody. Lane 1: NIH/3T3 whole cell lysate. Load: 20 ug per lane. Primary antibody: AKT antibody at 1:500 for overnight at 4C. Secondary antibody: HRP conjugated Gt-a-Rabbit IgG at 1:10000 preceded color development using Pierce Chemical's SuperSignal substrate. Block: MOPS buffer overnight at 4C. Predicted/Observed size: 128 kDa, 128 kDa for AKT. Other band(s): none.
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