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P4HA1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242305

MBS242305 is an antibody targeting P4HA1. Also known as Goat Polyclonal to Human P4HA1. Reactive in Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity). Host: Goat. More details

$680 / 0.05 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
P4HA1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human P4HA1
Product Gene Name
anti-P4HA1 antibody
Product Synonym Names
Anti-P4HA1 Antibody (Internal) IHC-plus; P4HA1; 4-PH alpha-1; C-P4Halpha(I); p4HA; p4HA1; Human P4HA1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human P4HA1. This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
58,958 Da
OMIM
176710
3D Structure
ModBase 3D Structure for P13674
Target Species
Human
Immunogen
P4HA1 antibody was raised against synthetic peptide C-DFARKDEPDAFKE from an internal region of human P4HA1 (NP_000908.2; NP_001017962.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%); Mouse, Rat, Hamster, Opossum, Salmon, Stickleback, Pufferfish, Zebrafish (92%).
Immunogen Type
Synthetic peptide
Applications Tested/Suitable for anti-P4HA1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-P4HA1 antibody
Immunohistochemistry: It was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

Immunohistochemistry (IHC) of anti-P4HA1 antibody

Anti-P4HA1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-P4HA1 Polyclonal Antibody – Cat# MBS242305

Immunohistochemistry (IHC) of anti-P4HA1 antibody

Anti-P4HA1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-P4HA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242305

Western Blot (WB) of anti-P4HA1 antibody

Antibody (0.2 ug/ml) staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-P4HA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242305

Other Notes

Small volumes of anti-P4HA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for P4HA1. It may not necessarily be applicable to this product.

NCBI GI #
63252886
NCBI GeneID
5033
NCBI Accession #
NP_000908.2
UniProt Primary Accession #
P13674
UniProt Secondary Accession #
Q15082; Q15083; Q5VSQ5; C9JL12
UniProt Related Accession #
P13674
GenBank Nucleotide #
NM_000917.3
NCBI Official Full Name
prolyl 4-hydroxylase subunit alpha-1 isoform 1
NCBI Official Synonym Full Names
prolyl 4-hydroxylase, alpha polypeptide I
NCBI Official Symbol
P4HA1
NCBI Official Synonym Symbols
P4HA
UniProt Protein Name
Prolyl 4-hydroxylase subunit alpha-1
UniProt Synonym Protein Names
Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1
Protein Family
Prolyl 4-hydroxylase
UniProt Gene Name
P4HA1
UniProt Synonym Gene Names
P4HA; 4-PH alpha-1
UniProt Entry Name
P4HA1_HUMAN
NCBI Protein Information
prolyl 4-hydroxylase subunit alpha-1; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I

NCBI Summary for P4HA1

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for P4HA1

P4HA1: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Belongs to the P4HA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Oxidoreductase; EC 1.14.11.2; Endoplasmic reticulum; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: intracellular membrane-bound organelle; membrane; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; procollagen-proline 4-dioxygenase activity; L-ascorbic acid binding; iron ion binding

Biological Process: collagen fibril organization; peptidyl-proline hydroxylation to 4-hydroxy-L-proline
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Research Articles on P4HA1

1. Overexpression of miR-122 markedly attenuated the expression of P4HA1 via targeting a binding site located at 3'-UTR of P4HA1 mRNA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-P4HA1 antibody

 Reference Product  PubMed Publications
 P4HB antibody  >2 publications with P4HA1 and P4HB

Pathways associated with anti-P4HA1 antibody

 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with anti-P4HA1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >4 publications with P4HA1 and Breast Neoplasms
 Inflammation Antibodies  >4 publications with P4HA1 and Inflammation
 Heart Diseases Antibodies  >2 publications with P4HA1 and Heart Diseases
 Fibrosis Antibodies  >2 publications with P4HA1 and Fibrosis
 Necrosis Antibodies  >2 publications with P4HA1 and Necrosis
 Disease Models, Animal Antibodies  >1 publications with P4HA1 and Disease Models, Animal
 Weight Loss Antibodies  >1 publications with P4HA1 and Weight Loss
 Liver Diseases Antibodies  >1 publications with P4HA1 and Liver Diseases

Organs/Tissues associated with anti-P4HA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >9 publications with P4HA1 and Vascular
 Muscle Antibodies  >6 publications with P4HA1 and Muscle
 Heart Antibodies  >4 publications with P4HA1 and Heart
 Liver Antibodies  >3 publications with P4HA1 and Liver
 Embryonic Tissue Antibodies  >3 publications with P4HA1 and Embryonic Tissue
 Bone Antibodies  >2 publications with P4HA1 and Bone
 Brain Antibodies  >2 publications with P4HA1 and Brain
 Prostate Antibodies  >1 publications with P4HA1 and Prostate
 Mouth Antibodies  >1 publications with P4HA1 and Mouth
 Connective Tissue Antibodies  >1 publications with P4HA1 and Connective Tissue
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