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VGF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS151354

VGF Antibody: VGF was initially identified as a rapidly regulated gene product in nerve growth factor-treated PC12 cells. This protein is synthesized in neurons in the central and peripheral nervous system as well as in the pituitary, adrenal medulla,… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
VGF, Polyclonal Antibody
Full Product Name
VGF Antibody
Product Gene Name
Product Synonym Names
VGF; Neurosecretory protein VGF; NERP-1; VGF nerve growth factor inducible
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid; VGF Antibody is supplied in PBS containing 0.02% sodium azide.
Purity/Purification
VGF Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
VGF peptide (MBS151855) is used for blocking the activity of VGF antibody (MBS151354)
Molecular Weight
Predicted: 68 kDa

Observed: 70 kDa
OMIM
602186
3D Structure
ModBase 3D Structure for O15240
Conjugate
Unconjugated
Immunogen
VGF antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human VGF.The immunogen is located within the last 50 amino acids of VGF.
Homology
Predicted Species reactivity based on immunogen sequence: Bovine: (100%)
Applications Tested/Suitable for anti-VGF antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-VGF antibody
VGF antibody can be used for detection of VGF by Western blot at 1-2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.

Western Blot (WB) of anti-VGF antibody

Western blot analysis of VGF expression in human brain tissue lysate with VGF antibody at (A) 1 and (B) 2 μg/ml.
Rabbit Anti-VGF Polyclonal Antibody – Cat# MBS151354

Immunohistochemistry (IHC) of anti-VGF antibody

Immunohistochemistry of VGF in human brain with VGFantibody at 5 μg/mL.
Rabbit Anti-VGF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS151354

Immunofluorescence (IF) of anti-VGF antibody

Immunofluorescence of VGF in Human Brain cells with VGF antibody at 20 μg/mL.
Rabbit Anti-VGF Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS151354

Other Notes

Small volumes of anti-VGF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VGF purchase

MBS151354 is an antibody targeting VGF. Also known as VGF Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VGF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neurosecretory protein VGF
NCBI Official Synonym Full Names
VGF nerve growth factor inducible
NCBI Official Symbol
VGF
UniProt Protein Name
Neurosecretory protein VGF
UniProt Gene Name
VGF
UniProt Synonym Gene Names
NERP-1; NERP-2
UniProt Entry Name
VGF_HUMAN
NCBI Protein Information
neurosecretory protein VGF; neuro-endocrine specific protein VGF

NCBI Summary for VGF

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]
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UniProt Comments for VGF

VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular space; transport vesicle; cytoplasmic vesicle

Molecular Function: neuropeptide hormone activity; growth factor activity

Biological Process: response to dietary excess; generation of precursor metabolites and energy; response to cAMP; ovarian follicle development; insulin secretion; sexual reproduction; defense response to bacterium; response to cold; glucose homeostasis; response to insulin stimulus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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