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GPX3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242329

MBS242329 is an antibody targeting GPX3. Also known as Goat Polyclonal to Human GPX3. Reactive in Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig. Host: Goat. More details

$680 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GPX3, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human GPX3
Product Gene Name
anti-GPX3 antibody
Product Synonym Names
Anti-GPX3 Antibody (Internal) IHC-plus; GPX3; Glutathione peroxidase 3; GSHPx-3; GPXP; GSHPx-P; GPx-3; GPx-P; Plasma glutathione peroxidase; Human GPX3
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml (lot specific)
Specificity
Human GPX3. This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
OMIM
138321
3D Structure
ModBase 3D Structure for P22352
Target Species
Human
Immunogen Description
Synthetic peptide C-NQFGKQEPGENSE from an internal region of human GPX3 (NP_002075.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig (100%).
Immunogen Type
Synthetic peptide
Immunogen
GPX3 antibody was raised against synthetic peptide C-NQFGKQEPGENSE from an internal region of human GPX3 (NP_002075.2).
Epitope
aa102-114
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-GPX3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-GPX3 antibody
IHC-p: 5ug/mL
WB: 1-3 ug/mL
Immunohistochemistry: MBS242329 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3036 was determined to be 5 ug/ml.

Immunohistochemistry (IHC) of anti-GPX3 antibody

Anti-GPX3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-GPX3 Polyclonal Antibody – Cat# MBS242329

Immunohistochemistry (IHC) of anti-GPX3 antibody

Anti-GPX3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-GPX3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242329

Western Blot (WB) of anti-GPX3 antibody

Antibody (1 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-GPX3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242329

Other Notes

Small volumes of anti-GPX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GPX3. It may not necessarily be applicable to this product.

NCBI GI #
6006001
NCBI GeneID
2878
NCBI Accession #
NP_002075.2
UniProt Primary Accession #
P22352
UniProt Secondary Accession #
O43787; Q86W78; Q9NZ74; Q9UEL1
UniProt Related Accession #
P22352
GenBank Nucleotide #
NM_002084.3
NCBI Official Full Name
glutathione peroxidase 3
NCBI Official Synonym Full Names
glutathione peroxidase 3 (plasma)
NCBI Official Symbol
GPX3
NCBI Official Synonym Symbols
GPx-P; GSHPx-3; GSHPx-P
UniProt Protein Name
Glutathione peroxidase 3
UniProt Synonym Protein Names
Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P
Protein Family
Probable glutathione peroxidase
UniProt Gene Name
GPX3
UniProt Synonym Gene Names
GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P
UniProt Entry Name
GPX3_HUMAN
NCBI Protein Information
glutathione peroxidase 3; GPx-3; plasma glutathione peroxidase; extracellular glutathione peroxidase

NCBI Summary for GPX3

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]
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UniProt Comments for GPX3

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.

Protein type: Oxidoreductase; Secreted; Secreted, signal peptide; Other Amino Acids Metabolism - glutathione; EC 1.11.1.9; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: extracellular space; extracellular region

Molecular Function: selenium binding; glutathione peroxidase activity; transcription factor binding

Biological Process: response to reactive oxygen species; response to lipid hydroperoxide; hydrogen peroxide catabolic process; protein homotetramerization
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Research Articles on GPX3

1. Our results indicate that promoter methylation is one of the major causes of GPX3 downregulation in cervical cancer and GPX3 could serve as a predictive biomarker for lymph node metastasis and prognosis of cervical cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GPX3 antibody

 Reference Product  PubMed Publications
 GSTM3 antibody  >4 publications with GPX3 and GSTM3
 GSR antibody  >4 publications with GPX3 and GSR
 GSTO1 antibody  >2 publications with GPX3 and GSTO1
 GSTA4 antibody  >2 publications with GPX3 and GSTA4
 GSTM5 antibody  >1 publications with GPX3 and GSTM5
 GSTM2 antibody  >1 publications with GPX3 and GSTM2
 GSTA3 antibody  >1 publications with GPX3 and GSTA3

Pathways associated with anti-GPX3 antibody

 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Detoxification Of Reactive Oxygen Species Pathway antibodies  Detoxification Of Reactive Oxygen Species Pathway Diagram
 Folate Metabolism Pathway antibodies  Folate Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Oxidative Stress Pathway antibodies  Oxidative Stress Pathway Diagram
 Selenium Metabolism And Selenoproteins Pathway antibodies  Selenium Metabolism And Selenoproteins Pathway Diagram

Diseases associated with anti-GPX3 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >27 publications with GPX3 and Cardiovascular Diseases
 Inflammation Antibodies  >23 publications with GPX3 and Inflammation
 Disease Models, Animal Antibodies  >20 publications with GPX3 and Disease Models, Animal
 Nervous System Diseases Antibodies  >17 publications with GPX3 and Nervous System Diseases
 Necrosis Antibodies  >15 publications with GPX3 and Necrosis
 Adenocarcinoma Antibodies  >14 publications with GPX3 and Adenocarcinoma
 Obesity Antibodies  >11 publications with GPX3 and Obesity
 Prostatic Neoplasms Antibodies  >11 publications with GPX3 and Prostatic Neoplasms
 Insulin Resistance Antibodies  >9 publications with GPX3 and Insulin Resistance
 Kidney Diseases Antibodies  >8 publications with GPX3 and Kidney Diseases

Organs/Tissues associated with anti-GPX3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >91 publications with GPX3 and Blood
 Liver Antibodies  >38 publications with GPX3 and Liver
 Kidney Antibodies  >29 publications with GPX3 and Kidney
 Muscle Antibodies  >24 publications with GPX3 and Muscle
 Lung Antibodies  >19 publications with GPX3 and Lung
 Thyroid Antibodies  >17 publications with GPX3 and Thyroid
 Prostate Antibodies  >15 publications with GPX3 and Prostate
 Heart Antibodies  >14 publications with GPX3 and Heart
 Brain Antibodies  >12 publications with GPX3 and Brain
 Adipose Tissue Antibodies  >11 publications with GPX3 and Adipose Tissue
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