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TSPO Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242336

MBS242336 is an antibody targeting TSPO. Also known as Goat Polyclonal to Human TSPO / PBR. Reactive in Human. Host: Goat. More details

$680 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TSPO / PBR, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human TSPO / PBR
Product Gene Name
anti-TSPO antibody
Product Synonym Gene Name
PBR
Product Synonym Names
Anti-TSPO / PBR Antibody (C-Terminus) IHC-plus; TSPO; BZRP; BPBS; IBP; MBR; MDRC; PBR; PBRS; PBS; PKBS; PTBR; Pk18; Translocator protein; Translocator protein (18kDa); Human TSPO; PBR
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Form/Format
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml (lot specific)
Specificity
Human TSPO. This antibody is expected to recognise one isoform (NP_000705.2) only.
Preparation and Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
OMIM
109610
3D Structure
ModBase 3D Structure for P30536
Epitope
aa156-169
Target
TSPO/ PBR
Immunogen Species
TSPO/PBR antibody was raised against Human
Immunogen
TSPO / PBR antibody was raised against peptide with sequence C-RDNHGWRGGRRLPE from the C-terminus of protein sequence according to NP_000705.2.
Usage
Immunohistochemistry: MBS242336 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3245 was determined to be 2.5 ug/ml.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-TSPO antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-TSPO antibody
IHC - Paraffin (2.5 µg/ml)
Western blot (0.1 - 0.3 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC) of anti-TSPO antibody

Anti-TSPO / PBR antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-TSPO Polyclonal Antibody – Cat# MBS242336

Immunohistochemistry (IHC) of anti-TSPO antibody

Anti-TSPO / PBR antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-TSPO Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242336

Western Blot (WB) of anti-TSPO antibody

Antibody (0.1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-TSPO Polyclonal Antibody, Western Blot (WB) – Cat# MBS242336

Other Notes

Small volumes of anti-TSPO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TSPO. It may not necessarily be applicable to this product.

NCBI GI #
74275350
NCBI GeneID
706
NCBI Accession #
NP_000705.2
UniProt Primary Accession #
P30536
UniProt Secondary Accession #
Q53Y59; Q6ICF9; Q96TF6
UniProt Related Accession #
P30536
GenBank Nucleotide #
NM_000714.5
NCBI Official Full Name
translocator protein
NCBI Official Synonym Full Names
translocator protein (18kDa)
NCBI Official Symbol
TSPO
NCBI Official Synonym Symbols
DBI; IBP; MBR; PBR; PBS; BPBS; BZRP; PKBS; PTBR; mDRC; pk18
UniProt Protein Name
Translocator protein
UniProt Synonym Protein Names
Mitochondrial benzodiazepine receptor; PKBS; Peripheral-type benzodiazepine receptor; PBR
Protein Family
Putative peripheral benzodiazepine receptor-related protein
UniProt Gene Name
TSPO
UniProt Synonym Gene Names
BZRP; MBR; PBR
UniProt Entry Name
TSPOA_HUMAN
NCBI Protein Information
translocator protein; mitochondrial benzodiazepine receptor; benzodiazepine peripheral binding site; peripheral-type benzodiazepine receptor

NCBI Summary for TSPO

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein. [provided by RefSeq, Feb 2012]
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UniProt Comments for TSPO

TSPO: Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. Belongs to the TspO/BZRP family. Interacts with BZRAP1. May interact with STAR. Interacts with MOST-1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Apoptosis; Receptor, misc.

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: mitochondrial outer membrane; nuclear membrane; intracellular membrane-bound organelle; mitochondrion; cytoplasm; integral to membrane

Molecular Function: androgen binding; benzodiazepine receptor activity; cholesterol binding

Biological Process: steroid metabolic process; apoptosis; positive regulation of apoptosis; regulation of steroid biosynthetic process; axon regeneration in the peripheral nervous system; anion transport; response to vitamin B1; synaptic transmission; behavioral response to pain; positive regulation of mitochondrial depolarization; protein targeting to mitochondrion; aging; response to drug; adrenal gland development; negative regulation of nitric oxide biosynthetic process; chloride transport; response to testosterone stimulus; negative regulation of tumor necrosis factor production; lipid transport; glial cell migration; response to manganese ion; cell proliferation; response to progesterone stimulus; positive regulation of calcium ion transport; regulation of cholesterol transport; steroid biosynthetic process; heme biosynthetic process
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Research Articles on TSPO

1. TSPO expression is able to distinguish differences in the severity of myocarditis between male subjects and female subjects.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TSPO antibody

 Products by Pathway  Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with anti-TSPO antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >118 publications with TSPO and Nervous System Diseases
 Brain Diseases Antibodies  >111 publications with TSPO and Brain Diseases
 Inflammation Antibodies  >95 publications with TSPO and Inflammation
 Peripheral Nervous System Diseases Antibodies  >32 publications with TSPO and Peripheral Nervous System Diseases
 Heart Diseases Antibodies  >23 publications with TSPO and Heart Diseases
 Kidney Diseases Antibodies  >16 publications with TSPO and Kidney Diseases
 Breast Neoplasms Antibodies  >12 publications with TSPO and Breast Neoplasms
 Liver Diseases Antibodies  >12 publications with TSPO and Liver Diseases
 Carcinoma Antibodies  >11 publications with TSPO and Carcinoma
 Necrosis Antibodies  >10 publications with TSPO and Necrosis

Organs/Tissues associated with anti-TSPO antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >230 publications with TSPO and Brain
 Blood Antibodies  >70 publications with TSPO and Blood
 Heart Antibodies  >46 publications with TSPO and Heart
 Kidney Antibodies  >35 publications with TSPO and Kidney
 Liver Antibodies  >30 publications with TSPO and Liver
 Testis Antibodies  >24 publications with TSPO and Testis
 Lung Antibodies  >23 publications with TSPO and Lung
 Nerve Antibodies  >21 publications with TSPO and Nerve
 Muscle Antibodies  >21 publications with TSPO and Muscle
 Spleen Antibodies  >15 publications with TSPO and Spleen
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