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ENO1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242359

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens… More details

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Product Overview

Product Name
ENO1 / Alpha Enolase, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human ENO1 / Alpha Enolase
Product Gene Name
anti-ENO1 antibody
Product Synonym Names
Anti-ENO1 / Alpha Enolase Antibody (Internal) IHC-plus; ENO1; Alpha Enolase; Alpha enolase like 1; ENO1L1; Enolase 1; MBP-1; MBPB1; MPB1; MYC promoter-binding protein 1; NNE; Phosphopyruvate hydratase; Alpha-enolase; Tau-crystallin; MBP1; MPB-1; C-myc promoter-binding protein; Enolase 1; (alpha); Human ENO1; Alpha Enolase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Ammonium sulfate precipitation
Concentration
0.25 mg/ml (lot specific)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Molecular Weight
36,928 Da
OMIM
172430
3D Structure
ModBase 3D Structure for P06733
Target Species
Human
Immunogen Description
KLH conjugated synthetic peptide selected from the Center region of human ENO1.
Immunogen Type
Synthetic peptide
Immunogen
ENO1 / Alpha Enolase antibody was raised against kLH conjugated synthetic peptide selected from the Center region of human ENO1.
Antigen Modification
Internal
Applications Tested/Suitable for anti-ENO1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-ENO1 antibody
Flo (1:10 - 1:50), IF (1:10 - 1:50), IHC-P (10 ug/ml), WB (1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-ENO1 antibody

Anti-ENO1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-ENO1 Polyclonal Antibody – Cat# MBS242359

Immunohistochemistry (IHC) of anti-ENO1 antibody

Anti-ENO1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-ENO1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242359

Immunohistochemistry (IHC) of anti-ENO1 antibody

Formalin-fixed and paraffin-embedded human lymph reacted with ENO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-ENO1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242359

Immunofluorescence (IF) of anti-ENO1 antibody

Fluorescent confocal image of C2C12 cell stained with ENO1 Antibody (Center).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENO1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ENO1 immunoreactivity is localized to Cytoplasm significantly.
Rabbit Anti-ENO1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS242359

Flow Cytometry (FC/FACS) of anti-ENO1 antibody

ENO1 Antibody (Center) flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ENO1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS242359

Western Blot (WB) of anti-ENO1 antibody

Western blot of ENO1 Antibody (Center) in Ramos, HL-60 cell line lysates (35 ug/lane). ENO1 (arrow) was detected using the purified Pab.
Rabbit Anti-ENO1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242359

Other Notes

Small volumes of anti-ENO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENO1 purchase

MBS242359 is an antibody targeting ENO1. Also known as Rabbit Polyclonal to Human ENO1 / Alpha Enolase. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ENO1. It may not necessarily be applicable to this product.

NCBI GI #
4503571
NCBI GeneID
2023
NCBI Accession #
NP_001419.1
UniProt Primary Accession #
P06733
UniProt Secondary Accession #
P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59
UniProt Related Accession #
P06733
GenBank Nucleotide #
NM_001428.3
NCBI Official Full Name
alpha-enolase isoform 1
NCBI Official Synonym Full Names
enolase 1, (alpha)
NCBI Official Symbol
ENO1
NCBI Official Synonym Symbols
NNE; PPH; MPB1; ENO1L1
UniProt Protein Name
Alpha-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein
Protein Family
Alpha-enolase
UniProt Gene Name
ENO1
UniProt Synonym Gene Names
ENO1L1; MBPB1; MPB1; NNE
UniProt Entry Name
ENOA_HUMAN
NCBI Protein Information
alpha-enolase; c-myc promoter-binding protein-1; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase

NCBI Summary for ENO1

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
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UniProt Comments for ENO1

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: EC 4.2.1.11; Transcription, coactivator/corepressor; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: extracellular space; phosphopyruvate hydratase complex; membrane; cytoplasm; plasma membrane; M band; cytosol; nucleus

Molecular Function: protein binding; DNA binding; magnesium ion binding; phosphopyruvate hydratase activity; transcription corepressor activity; transcription factor activity

Biological Process: glycolysis; transcription, DNA-dependent; response to virus; carbohydrate metabolic process; glucose metabolic process; pathogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; gluconeogenesis
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Research Articles on ENO1

1. Isotypes of anti-citrullinated fibrinogen and alpha-enolase can be found in the serum of children with juvenile idiopathic arthritis of all onset types.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ENO1 antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram

Diseases associated with anti-ENO1 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >38 publications with ENO1 and Carcinoma
 Nervous System Diseases Antibodies  >20 publications with ENO1 and Nervous System Diseases
 Arthritis, Rheumatoid Antibodies  >14 publications with ENO1 and Arthritis, Rheumatoid
 Adenocarcinoma Antibodies  >13 publications with ENO1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >13 publications with ENO1 and Breast Neoplasms
 Carcinoma, Non-Small-Cell Lung Antibodies  >12 publications with ENO1 and Carcinoma, Non-Small-Cell Lung
 Inflammation Antibodies  >12 publications with ENO1 and Inflammation
 Neoplasms, Experimental Antibodies  >9 publications with ENO1 and Neoplasms, Experimental
 Carcinoma, Squamous Cell Antibodies  >7 publications with ENO1 and Carcinoma, Squamous Cell
 Liver Neoplasms Antibodies  >7 publications with ENO1 and Liver Neoplasms

Organs/Tissues associated with anti-ENO1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >50 publications with ENO1 and Blood
 Lung Antibodies  >31 publications with ENO1 and Lung
 Brain Antibodies  >31 publications with ENO1 and Brain
 Liver Antibodies  >17 publications with ENO1 and Liver
 Muscle Antibodies  >15 publications with ENO1 and Muscle
 Eye Antibodies  >12 publications with ENO1 and Eye
 Vascular Antibodies  >12 publications with ENO1 and Vascular
 Kidney Antibodies  >11 publications with ENO1 and Kidney
 Nerve Antibodies  >9 publications with ENO1 and Nerve
 Ovary Antibodies  >8 publications with ENO1 and Ovary
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