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ANXA1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242449

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35000 to 40000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent… More details

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Product Overview

Online Datasheet in PDF
Product Name
ANXA1 / Annexin A1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human ANXA1 / Annexin A1
Product Gene Name
anti-ANXA1 antibody
Product Synonym Names
Anti-ANXA1 / Annexin A1 Antibody (C-Terminus) IHC-plus; ANXA1; Annexin A1; Annexin I; Annexin-1; ANX1; Calpactin II; Chromobindin-9; Lipocortin I; Annexin I (lipocortin I); Calpactin-2; LPC1; Human ANXA1; Annexin A1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea Pig, Hamster, Horse, Pig, Rabbit
Form/Format
TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
Recommended Concentration: 0.5-1.5 mg/ml (lot specific)
Specificity
Human ANXA1 / Annexin A1.
Preparation and Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Molecular Weight
38,714 Da
OMIM
151690
3D Structure
ModBase 3D Structure for P04083
Immunogen Species
Human
Immunogen
ANXA1 / Annexin A1 antibody was raised against peptide with sequence CQAILDETKGDYEK from the C-terminus of protein sequence according to (NP_000691.1).
Epitope
aa324-337
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-ANXA1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ANXA1 antibody
ELISA (1:32000), IHC-P (4 ug/ml), WB (1-3ug/mL), Peptide Enzyme-Linked Immunoabsorbent Assay (1:4000)
Optimal dilution should be determined by the researcher.
Usage: Immunohistochemistry: MBS242474 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242474 was determined to be 4 ug/ml.
Peptide ELISA: antibody detection limit dilution 1:3200.
Western Blot: Approx 35kD band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7 kD according to NP_000691.1).
The observed molecular weight corresponds to earlier findings in literature with different antibodies ( Paweletz et al, Cancer RES. 2000 Nov15;16(22):6293-7. pmid: 11103786).

Immunohistochemistry (IHC) of anti-ANXA1 antibody

Anti-Annexin A1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.
Goat Anti-ANXA1 Polyclonal Antibody – Cat# MBS242449

Immunohistochemistry (IHC) of anti-ANXA1 antibody

Anti-Annexin A1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.
Goat Anti-ANXA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242449

Western Blot (WB) of anti-ANXA1 antibody

Antibody (0.5 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ANXA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242449

Other Notes

Small volumes of anti-ANXA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ANXA1 purchase

MBS242449 is an antibody targeting ANXA1. Also known as Goat Polyclonal to Human ANXA1 / Annexin A1. Reactive in Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea Pig, Hamster, Horse, Pig, Rabbit. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for ANXA1. It may not necessarily be applicable to this product.

NCBI GI #
4502101
NCBI GeneID
301
NCBI Accession #
NP_000691.1
UniProt Primary Accession #
P04083
UniProt Related Accession #
P04083
GenBank Nucleotide #
NM_000700.2
NCBI Official Full Name
annexin A1
NCBI Official Synonym Full Names
annexin A1
NCBI Official Symbol
ANXA1
NCBI Official Synonym Symbols
ANX1; LPC1
UniProt Protein Name
Annexin A1
UniProt Synonym Protein Names
Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35
Protein Family
Annexin
UniProt Gene Name
ANXA1
UniProt Synonym Gene Names
ANX1; LPC1
UniProt Entry Name
ANXA1_HUMAN
NCBI Protein Information
annexin A1; annexin-1; calpactin-2; calpactin II; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein

NCBI Summary for ANXA1

This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]

UniProt Comments for ANXA1

ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: extracellular space; mast cell granule; protein complex; cell surface; focal adhesion; basolateral plasma membrane; extracellular region; cornified envelope; cilium; mitochondrial membrane; cytoplasm; plasma membrane; nucleus; sarcolemma; vesicle; endosome

Molecular Function: protein binding, bridging; protein binding; single-stranded RNA binding; protein homodimerization activity; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; double-stranded DNA-dependent ATPase activity; phospholipid binding; calcium ion binding; structural molecule activity; helicase activity; calcium-dependent protein binding; single-stranded DNA binding; receptor binding

Biological Process: prostate gland development; response to drug; response to peptide hormone stimulus; positive regulation of neutrophil apoptosis; gliogenesis; positive regulation of vesicle fusion; alpha-beta T cell differentiation; arachidonic acid secretion; signal transduction; neutrophil homeostasis; endocrine pancreas development; response to estradiol stimulus; DNA duplex unwinding; regulation of cell proliferation; keratinocyte differentiation; cell surface receptor linked signal transduction; insulin secretion; DNA strand renaturation; peptide cross-linking; cell motility; inflammatory response; negative regulation of apoptosis; positive regulation of prostaglandin biosynthetic process; response to X-ray
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Research Articles on ANXA1

1. result of the present study suggest that ANXA1 complex coordinates the actions of PKA and PKC to control the distribution of oxytocin vesicles between the axon and the cell body

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ANXA1 antibody

 Reference Product  PubMed Publications
 FPR2 antibody  >24 publications with ANXA1 and FPR2
 FPR1 antibody  >14 publications with ANXA1 and FPR1
 POMC antibody  >11 publications with ANXA1 and POMC
 S100A11 antibody  >5 publications with ANXA1 and S100A11
 FPR3 antibody  >3 publications with ANXA1 and FPR3
 S100A6 antibody  >2 publications with ANXA1 and S100A6

Pathways associated with anti-ANXA1 antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway antibodies  Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram

Diseases associated with anti-ANXA1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >79 publications with ANXA1 and Inflammation
 Carcinoma Antibodies  >57 publications with ANXA1 and Carcinoma
 Disease Models, Animal Antibodies  >25 publications with ANXA1 and Disease Models, Animal
 Brain Diseases Antibodies  >19 publications with ANXA1 and Brain Diseases
 Neoplasm Metastasis Antibodies  >18 publications with ANXA1 and Neoplasm Metastasis
 Carcinoma, Squamous Cell Antibodies  >17 publications with ANXA1 and Carcinoma, Squamous Cell
 Lung Diseases Antibodies  >16 publications with ANXA1 and Lung Diseases
 Cardiovascular Diseases Antibodies  >12 publications with ANXA1 and Cardiovascular Diseases
 Necrosis Antibodies  >12 publications with ANXA1 and Necrosis
 Stomach Diseases Antibodies  >10 publications with ANXA1 and Stomach Diseases

Organs/Tissues associated with anti-ANXA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >90 publications with ANXA1 and Blood
 Brain Antibodies  >29 publications with ANXA1 and Brain
 Lung Antibodies  >23 publications with ANXA1 and Lung
 Vascular Antibodies  >21 publications with ANXA1 and Vascular
 Liver Antibodies  >15 publications with ANXA1 and Liver
 Pituitary Gland Antibodies  >13 publications with ANXA1 and Pituitary Gland
 Stomach Antibodies  >11 publications with ANXA1 and Stomach
 Lymph Node Antibodies  >11 publications with ANXA1 and Lymph Node
 Bone Antibodies  >10 publications with ANXA1 and Bone
 Muscle Antibodies  >10 publications with ANXA1 and Muscle
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