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PLIN1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242628

MBS242628 is an antibody targeting PLIN1. Also known as Goat Polyclonal to Human PLIN1 / Perilipin. Reactive in Bovine, Dog, Horse, Human, Monkey, MousePredicted Reactivity: Gorilla, Gibbon, Rat, Bat, Hamster, Pig (at least 90% immunogen sequence identity).… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PLIN1 / Perilipin, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human PLIN1 / Perilipin
Product Gene Name
Product Synonym Names
Anti-PLIN1 / Perilipin Antibody (C-Terminus) IHC-plus; PLIN1; FPLD4; PERI; Perilipin-1; PLIN; Perilipin; Perilipin 1; Human PLIN1; Perilipin
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bovine, Dog, Horse, Human, Monkey, Mouse
Predicted Reactivity: Gorilla, Gibbon, Rat, Bat, Hamster, Pig (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human PLIN1 / Perilipin.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
55,990 Da
OMIM
gene 613877
3D Structure
ModBase 3D Structure for O60240
Target Species
Human
Immunogen Description
Synthetic peptide C-EPILGRAQYSQLRKK from the C-terminus of human PLIN1 / Perilipin (NP_002657.2). Percent identity by BLAST analysis: Monkey, Marmoset, Mouse, Panda, Bovine, Dog, Horse (100%); Gorilla, Gibbon, Rat, Hamster, Elephant, Bat, Pig (93%).
Immunogen Type
Synthetic peptide
Immunogen
PLIN1 / Perilipin antibody was raised against synthetic peptide C-EPILGRAQYSQLRKK from the C-terminus of human PLIN1 / Perilipin (NP_002657.2). Percent identity by BLAST analysis: Monkey, Marmoset, Mouse, Panda, Bovine, Dog, Horse (100%); Gorilla, Gibbon, Rat, Hamster, Elephant, Bat, Pig (93%); Human (87%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-PLIN1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PLIN1 antibody
ELISA (1:32000), IHC-P (4 ug/ml), WB (0.01 - 0.03 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-PLIN1 antibody

Anti-Perilipin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.
Goat Anti-PLIN1 Polyclonal Antibody – Cat# MBS242628

Western Blot (WB) of anti-PLIN1 antibody

Antibody (0.01 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PLIN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242628

Other Notes

Small volumes of anti-PLIN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PLIN1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
perilipin-1
NCBI Official Synonym Full Names
perilipin 1
NCBI Official Symbol
PLIN1
NCBI Official Synonym Symbols
PERI; PLIN; FPLD4
UniProt Protein Name
Perilipin-1
UniProt Synonym Protein Names
Lipid droplet-associated protein
Protein Family
UniProt Gene Name
PLIN1
UniProt Synonym Gene Names
PERI; PLIN
UniProt Entry Name
PLIN1_HUMAN
NCBI Protein Information
perilipin-1; lipid droplet-associated protein

NCBI Summary for PLIN1

The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009]
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UniProt Comments for PLIN1

perilipin 1: an adipocyte protein of the peripilin family. A modulator of adipocyte lipid metabolism, coating lipid storage droplets to protect them from being broken down by hormone-sensitive lipase. A major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: endoplasmic reticulum; lipid particle; cytosol

Molecular Function: lipid binding

Biological Process: triacylglycerol catabolic process; lipid metabolic process

Disease: Lipodystrophy, Familial Partial, Type 4
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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