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GAD67 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242634

Immunohistochemistry: MBS242634 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody,… More details

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Product Overview

Online Datasheet in PDF
Product Name
GAD67, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human GAD67
Product Gene Name
anti-GAD67 antibody
Product Synonym Gene Name
GAD1
Product Synonym Names
Anti-GAD67 Antibody (Internal) IHC-plus; GAD1; CPSQ1; GAD-67; Glutamate decarboxylase 1; GAD67; GAD; SCP; Human GAD67
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human, Mouse
Form/Format
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml (lot specific)
Specificity
Human GAD11 GAD67. This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
Preparation and Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Molecular Weight
47,440 Da
Immunogen Sequence Positions
526-537
OMIM
phenotype 605363
3D Structure
ModBase 3D Structure for Q99259
Immunogen Species
GAD1 1 GAD67 antibody was raised against Human
Immunogen
GAD1 1 GAD67 antibody was raised against peptide with sequence CPDSPQRREKLHK, from the internal region of the protein sequence according to NP _000808.2.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect tohow this antibody will behave in your system. Researchers using this antibody should conduct optimization studies to achieve themost optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes noguarantee of the results:Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-GAD67 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-GAD67 antibody
IHC - Paraffin (4 IJg/ml)
Western blot (0.5 - 1.5 ug/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
(optimal dilution to be determined by the researcher)

Immunohistochemistry (IHC) of anti-GAD67 antibody

Anti-GAD67 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.
Goat Anti-GAD67 Polyclonal Antibody – Cat# MBS242634

Western Blot (WB) of anti-GAD67 antibody

Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-GAD67 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242634

Other Notes

Small volumes of anti-GAD67 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAD67 purchase

MBS242634 is an antibody targeting GAD67. Also known as Goat Polyclonal to Human GAD67. Reactive in Human, Mouse. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for GAD67. It may not necessarily be applicable to this product.

NCBI GI #
58331246
NCBI GeneID
2571
NCBI Accession #
NP_000808.2
UniProt Primary Accession #
Q99259
UniProt Secondary Accession #
Q49AK1; Q53TQ7; Q9BU91; Q9UHH4
UniProt Related Accession #
Q99259
GenBank Nucleotide #
NM_000817.2
NCBI Official Full Name
glutamate decarboxylase 1 isoform GAD67
NCBI Official Synonym Full Names
glutamate decarboxylase 1 (brain, 67kDa)
NCBI Official Symbol
GAD1
NCBI Official Synonym Symbols
GAD; SCP; CPSQ1
UniProt Protein Name
Glutamate decarboxylase 1
UniProt Synonym Protein Names
67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
UniProt Gene Name
GAD1
UniProt Synonym Gene Names
GAD; GAD67; GAD-67
UniProt Entry Name
DCE1_HUMAN
NCBI Protein Information
glutamate decarboxylase 1; GAD-67; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform

NCBI Summary for GAD67

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
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UniProt Comments for GAD67

GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Other Amino Acids Metabolism - taurine and hypotaurine; EC 4.1.1.15; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Lyase; Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - beta-alanine

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: plasma membrane; vesicle membrane; intracellular

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate catabolic process; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process

Disease: Cerebral Palsy, Spastic Quadriplegic, 1
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Research Articles on GAD67

1. CNR1, GAD1 and BDNF polymorphisms are associated with male heroin dependence in the Dai population in China.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GAD67 antibody

 Reference Product  PubMed Publications
 GAD2 antibody  >50 publications with GAD67 and GAD2
 GLUL antibody  >2 publications with GAD67 and GLUL
 OLIG2 antibody  >2 publications with GAD67 and OLIG2
 ABAT antibody  >1 publications with GAD67 and ABAT

Pathways associated with anti-GAD67 antibody

 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA Synthesis Pathway antibodies  GABA Synthesis Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram

Diseases associated with anti-GAD67 antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >55 publications with GAD67 and Mental Disorders
 Schizophrenia Antibodies  >40 publications with GAD67 and Schizophrenia
 Nervous System Diseases Antibodies  >29 publications with GAD67 and Nervous System Diseases
 Brain Diseases Antibodies  >23 publications with GAD67 and Brain Diseases
 Disease Models, Animal Antibodies  >15 publications with GAD67 and Disease Models, Animal
 Seizures Antibodies  >9 publications with GAD67 and Seizures
 Anxiety Disorders Antibodies  >8 publications with GAD67 and Anxiety Disorders
 Autistic Disorder Antibodies  >6 publications with GAD67 and Autistic Disorder
 Depressive Disorder Antibodies  >6 publications with GAD67 and Depressive Disorder
 Cognition Disorders Antibodies  >4 publications with GAD67 and Cognition Disorders

Organs/Tissues associated with anti-GAD67 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >101 publications with GAD67 and Brain
 Embryonic Tissue Antibodies  >2 publications with GAD67 and Embryonic Tissue
 Muscle Antibodies  >2 publications with GAD67 and Muscle
 Eye Antibodies  >2 publications with GAD67 and Eye
 Kidney Antibodies  >2 publications with GAD67 and Kidney
 Bone Antibodies  >1 publications with GAD67 and Bone
 Thymus Antibodies  >1 publications with GAD67 and Thymus
 Uncharacterized Tissue Antibodies  >1 publications with GAD67 and Uncharacterized Tissue
 Intestine Antibodies  >1 publications with GAD67 and Intestine
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