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HEC1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242638

MBS242638 is an antibody targeting HEC1. Also known as Goat Polyclonal to Human HEC1 / NDC80. Reactive in Gorilla, HumanPredicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Overview

Product Name
HEC1 / NDC80, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human HEC1 / NDC80
Product Gene Name
anti-HEC1 antibody
Product Synonym Gene Name
NDC80
Product Synonym Names
Anti-HEC1 / NDC80 Antibody (C-Terminus) IHC-plus; NDC80; HEC1; Kinetochore protein Hec1; Kinetochore associated 2; HEC; HsHec1; HsNDC80; KNTC2; TID3; Human HEC1; NDC80
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gorilla, Human
Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human NDC80 / HEC1.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
73,913 Da
OMIM
607272
3D Structure
ModBase 3D Structure for O14777
Target Species
Human
Immunogen Description
Synthetic peptide C-YEKKATLIKSSEE from the C-terminus of human NDC80 / HEC1 (NP_006092.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (92%).
Immunogen Type
Synthetic peptide
Immunogen
HEC1 / NDC80 antibody was raised against synthetic peptide C-YEKKATLIKSSEE from the C-terminus of human NDC80 / HEC1 (NP_006092.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (92%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-HEC1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-HEC1 antibody
ELISA (1:32000), IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-HEC1 antibody

Anti-NDC80 / HEC1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-HEC1 Polyclonal Antibody – Cat# MBS242638

Immunohistochemistry (IHC) of anti-HEC1 antibody

Anti-NDC80 / HEC1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-HEC1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242638

Western Blot (WB) of anti-HEC1 antibody

Antibody staining (0.5 ug/ml) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Goat Anti-HEC1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242638

Other Notes

Small volumes of anti-HEC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HEC1. It may not necessarily be applicable to this product.

NCBI GI #
5174457
NCBI GeneID
10403
NCBI Accession #
NP_006092.1
UniProt Primary Accession #
O14777
UniProt Secondary Accession #
Q6PJX2
UniProt Related Accession #
O14777
GenBank Nucleotide #
NM_006101.2
NCBI Official Full Name
kinetochore protein NDC80 homolog
NCBI Official Synonym Full Names
NDC80 kinetochore complex component
NCBI Official Symbol
NDC80
NCBI Official Synonym Symbols
HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
UniProt Protein Name
Kinetochore protein NDC80 homolog
UniProt Synonym Protein Names
Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC
Protein Family
Transcription factor
UniProt Gene Name
NDC80
UniProt Synonym Gene Names
HEC; HEC1; KNTC2; HsHec1
UniProt Entry Name
NDC80_HUMAN
NCBI Protein Information
kinetochore protein NDC80 homolog; kinetochore associated 2; kinetochore protein Hec1; kinetochore-associated protein 2; highly expressed in cancer protein; retinoblastoma-associated protein HEC; NDC80 kinetochore complex component homolog; NDC80 homolog, kinetochore complex component; highly expressed in cancer, rich in leucine heptad repeats

NCBI Summary for HEC1

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
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UniProt Comments for HEC1

HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: kinetochore; membrane; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: mitosis; mitotic spindle organization and biogenesis; mitotic sister chromatid segregation; attachment of spindle microtubules to kinetochore during mitosis; establishment of mitotic spindle orientation; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; chromosome segregation
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Research Articles on HEC1

1. Hec1 is critical in maintaining the in vitro and in vivo growth of gastric cancer cells; elevated Hec1 levels may occur at the early stage of gastric tumorigenesis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HEC1 antibody

 Products by Pathway  Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
 Mitotic Metaphase And Anaphase Pathway antibodies  Mitotic Metaphase And Anaphase Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 PLK1 Signaling Events Pathway antibodies  PLK1 Signaling Events Pathway Diagram
 Resolution Of Sister Chromatid Cohesion Pathway antibodies  Resolution Of Sister Chromatid Cohesion Pathway Diagram
 Separation Of Sister Chromatids Pathway antibodies  Separation Of Sister Chromatids Pathway Diagram

Diseases associated with anti-HEC1 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >8 publications with HEC1 and Carcinoma
 Ovarian Neoplasms Antibodies  >5 publications with HEC1 and Ovarian Neoplasms
 Lung Neoplasms Antibodies  >5 publications with HEC1 and Lung Neoplasms
 Breast Neoplasms Antibodies  >5 publications with HEC1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >4 publications with HEC1 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >4 publications with HEC1 and Cell Transformation, Neoplastic
 Disease Models, Animal Antibodies  >3 publications with HEC1 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >3 publications with HEC1 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >2 publications with HEC1 and Nervous System Diseases
 Liver Neoplasms Antibodies  >2 publications with HEC1 and Liver Neoplasms

Organs/Tissues associated with anti-HEC1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with HEC1 and Blood
 Lung Antibodies  >5 publications with HEC1 and Lung
 Brain Antibodies  >4 publications with HEC1 and Brain
 Heart Antibodies  >3 publications with HEC1 and Heart
 Stomach Antibodies  >3 publications with HEC1 and Stomach
 Liver Antibodies  >2 publications with HEC1 and Liver
 Prostate Antibodies  >1 publications with HEC1 and Prostate
 Cervix Antibodies  >1 publications with HEC1 and Cervix
 Ovary Antibodies  >1 publications with HEC1 and Ovary
 Skin Antibodies  >1 publications with HEC1 and Skin
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