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PIM3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242649

KID-1 is an immediate early gene in the PIM protein kinase subfamily. KID-1 is induced by depolarization in PC12 cells and in brain. In brain, KID-1 expression is induced after generalized seizures and is limited to specific areas of the hippocampus and… More details

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Product Overview

Product Name
PIM3 / PIM-3, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human PIM3 / PIM-3
Product Gene Name
Product Synonym Gene Name
PIM-3
Product Synonym Names
Anti-PIM3 / PIM-3 Antibody (C-Terminus) IHC-plus; PIM3; KID1; Pim-3 oncogene; Serine/threonine kinase Pim-3; Pim-3; Human PIM3; PIM-3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Ammonium sulfate precipitation
Concentration
0.25 mg/ml (lot specific)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Molecular Weight
35,891 Da
OMIM
610580
3D Structure
ModBase 3D Structure for Q86V86
Target Species
Human
Immunogen Description
KLH conjugated synthetic peptide selected from the C-terminal region of human PIM3.
Immunogen Type
Synthetic peptide
Immunogen
PIM3 / PIM-3 antibody was raised against kLH conjugated synthetic peptide selected from the C-terminal region of human PIM3.
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-PIM3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-PIM3 antibody
IHC-P (10 ug/ml), WB (1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-PIM3 antibody

Anti-PIM3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-PIM3 Polyclonal Antibody – Cat# MBS242649

Immunohistochemistry (IHC) of anti-PIM3 antibody

Formalin-fixed and paraffin-embedded human Spleen tissue reacted with PIM3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PIM3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242649

Immunohistochemistry (IHC) of anti-PIM3 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIM3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PIM3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242649

Western Blot (WB) of anti-PIM3 antibody

is used in Western blot to detect PIM3 in mouse kidney tissue lysate.
Rabbit Anti-PIM3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242649

Western Blot (WB) of anti-PIM3 antibody

The anti-PIM3 Pab is used in Western blot to detect PIM3 in mouse kidney tissue lysate.
Rabbit Anti-PIM3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242649

Other Notes

Small volumes of anti-PIM3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIM3 purchase

MBS242649 is an antibody targeting PIM3. Also known as Rabbit Polyclonal to Human PIM3 / PIM-3. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PIM3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase pim-3
NCBI Official Synonym Full Names
Pim-3 proto-oncogene, serine/threonine kinase
NCBI Official Symbol
PIM3
NCBI Official Synonym Symbols
pim-3
UniProt Protein Name
Serine/threonine-protein kinase pim-3
UniProt Gene Name
PIM3
UniProt Entry Name
PIM3_HUMAN
NCBI Protein Information
serine/threonine-protein kinase pim-3; pim-3 oncogene; serine/threonine kinase Pim-3

NCBI Summary for PIM3

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis. [provided by RefSeq, Jun 2012]
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UniProt Comments for PIM3

Pim3: a Ca(2 )/calmodulin-dependent protein kinase of the PIM family. Highly expressed in hematopoietic tissues, in leukemia and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, CAMK; Oncoprotein; CAMK group; PIM family

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: apoptosis; histone phosphorylation; protein amino acid autophosphorylation; regulation of mitotic cell cycle; cell cycle; protein amino acid phosphorylation; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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