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MCAM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242650

MCAM (also termed CD146 or MUC18) is a cell-adhesion molecule belonging to the immunoglobulin gene superfamily. It mediates cell-cell interaction through heterophilic Mel-CAM/ligand adhesion. MCAM has been shown to expressed widely in normal and neoplastic… More details

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Product Overview

Product Name
MCAM / CD146, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human MCAM / CD146
Product Gene Name
anti-MCAM antibody
Product Synonym Gene Name
CD146
Product Synonym Names
Anti-MCAM / CD146 Antibody (Tyr641) IHC-plus; MCAM; CD146; CD146 antigen; Gicerin; Melanoma adhesion molecule; MUC18; p1h12; Human MCAM; CD146
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.25 mg/ml (lot specific)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Molecular Weight
57,605 Da
OMIM
155735
3D Structure
ModBase 3D Structure for P43121
Target Species
Human
Immunogen Description
KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y641 of human MCAM.
Immunogen Type
Synthetic peptide
Immunogen
MCAM / CD146 antibody was raised against kLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y641 of human MCAM.
Antigen Modification
Tyr641

Product Categories/Family for anti-MCAM antibody

Family: Immunoglobulin
Applications Tested/Suitable for anti-MCAM antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-MCAM antibody
IHC-P (10 ug/ml), WB (1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-MCAM antibody

Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-MCAM Polyclonal Antibody – Cat# MBS242650

Western Blot (WB) of anti-MCAM antibody

Western blot analysis of anti-MCAM Antibody (Y641) in A375 cell line lysates (35 ug/lane). MCAM (arrow) was detected using the purified Pab.
Rabbit Anti-MCAM Polyclonal Antibody, Western Blot (WB) – Cat# MBS242650

Other Notes

Small volumes of anti-MCAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCAM purchase

MBS242650 is an antibody targeting MCAM. Also known as Rabbit Polyclonal to Human MCAM / CD146. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MCAM. It may not necessarily be applicable to this product.

NCBI GI #
71274107
NCBI GeneID
4162
NCBI Accession #
NP_006491.2
UniProt Primary Accession #
P43121
UniProt Secondary Accession #
O95812; Q59E86; Q6PHR3; Q6ZTR2
UniProt Related Accession #
P43121
GenBank Nucleotide #
NM_006500.2
NCBI Official Full Name
cell surface glycoprotein MUC18
NCBI Official Synonym Full Names
melanoma cell adhesion molecule
NCBI Official Symbol
MCAM
NCBI Official Synonym Symbols
CD146; MUC18
UniProt Protein Name
Cell surface glycoprotein MUC18
UniProt Synonym Protein Names
Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD_antigen: CD146
Protein Family
Cell surface glycoprotein
UniProt Gene Name
MCAM
UniProt Synonym Gene Names
MUC18
UniProt Entry Name
MUC18_HUMAN
NCBI Protein Information
cell surface glycoprotein MUC18; Gicerin; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen

UniProt Comments for MCAM

MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; focal adhesion; integral to membrane; plasma membrane; nucleus; external side of plasma membrane

Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration
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Research Articles on MCAM

1. CD146 and its soluble form correlate with the development of inflammation of atherosclerosis and plaque instability; CD146 may be a promising biomarker for monitoring the development and instability of atherosclerotic plaque in carotid disease patients

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MCAM antibody

 Reference Product  PubMed Publications
 CD34 antibody  >23 publications with MCAM and CD34
 PROM1 antibody  >2 publications with MCAM and PROM1
 FYN antibody  >2 publications with MCAM and FYN
 SELE antibody  >1 publications with MCAM and SELE
 EDN1 antibody  >1 publications with MCAM and EDN1

Diseases associated with anti-MCAM antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >29 publications with MCAM and Inflammation
 Neoplasms, Experimental Antibodies  >24 publications with MCAM and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >23 publications with MCAM and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >22 publications with MCAM and Disease Models, Animal
 Adenocarcinoma Antibodies  >22 publications with MCAM and Adenocarcinoma
 Lung Neoplasms Antibodies  >20 publications with MCAM and Lung Neoplasms
 Breast Neoplasms Antibodies  >18 publications with MCAM and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >16 publications with MCAM and Prostatic Neoplasms
 Neovascularization, Pathologic Antibodies  >15 publications with MCAM and Neovascularization, Pathologic
 Nervous System Diseases Antibodies  >15 publications with MCAM and Nervous System Diseases

Organs/Tissues associated with anti-MCAM antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >120 publications with MCAM and Blood
 Vascular Antibodies  >101 publications with MCAM and Vascular
 Bone Antibodies  >48 publications with MCAM and Bone
 Skin Antibodies  >43 publications with MCAM and Skin
 Lung Antibodies  >40 publications with MCAM and Lung
 Embryonic Tissue Antibodies  >36 publications with MCAM and Embryonic Tissue
 Bone Marrow Antibodies  >34 publications with MCAM and Bone Marrow
 Muscle Antibodies  >30 publications with MCAM and Muscle
 Prostate Antibodies  >19 publications with MCAM and Prostate
 Brain Antibodies  >17 publications with MCAM and Brain
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