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PPP4C Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242662

Protein phosphatase 4 (protein phosphatase X) is a 35kD serine/threonine phosphatase with predicted binding sites for iron and manganese. It is found in both the cytoplasm and the nucleus. The phophoprotein alpha 4 has been identified as a potential… More details

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Product Overview

Product Name
PPP4C, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human PPP4C
Product Gene Name
Product Synonym Names
Anti-PPP4C Antibody IHC-plus; PPP4C; PP4; PPH3; Protein phosphatase X; PP-X; Protein phosphatase 4; PP4C; PPP4; PPX; Human PPP4C
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bat, Gibbon, Bovine, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Rabbit, Rat, Zebrafish
Predicted Reactivity: Drosophila, Insect (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human PPP4C.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
35,080 Da
OMIM
602035
3D Structure
ModBase 3D Structure for P60510
Target Species
Human
Immunogen Description
Synthetic peptide C-PQETRGIPSKKP from the C-terminus of human PPP4C (NP_002711.1).
Immunogen Type
Synthetic peptide
Immunogen
PPP4C antibody was raised against synthetic peptide C-PQETRGIPSKKP from the C-terminus of human PPP4C (NP_002711.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus, Xenopus, Catfish, Stickleback, Pufferfish, Zebrafish, Water flea (100%); Drosophila, Mosquito, Ant, Beetle (92%); Seq squirt, Poplar, Trichoplax, Toxoplasma, Tick, Spikemoss, Amoeba, Neospora (83%).
Applications Tested/Suitable for anti-PPP4C antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PPP4C antibody
ELISA, IHC-P (2.5 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-PPP4C antibody

Anti-PPP4C antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Goat Anti-PPP4C Polyclonal Antibody – Cat# MBS242662

Immunohistochemistry (IHC) of anti-PPP4C antibody

Anti-PPP4C antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Goat Anti-PPP4C Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS242662

Western Blot (WB) of anti-PPP4C antibody

Antibody (0.3 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PPP4C Polyclonal Antibody, Western Blot (WB) – Cat# MBS242662

Other Notes

Small volumes of anti-PPP4C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPP4C purchase

MBS242662 is an antibody targeting PPP4C. Also known as Goat Polyclonal to Human PPP4C. Reactive in Bat, Gibbon, Bovine, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Rabbit, Rat, Zebrafish
Predicted Reactivity: Drosophila, Insect (at least 90% immunogen sequence identity). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP4C. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein phosphatase 4 catalytic subunit
NCBI Official Synonym Full Names
protein phosphatase 4, catalytic subunit
NCBI Official Symbol
PPP4C
NCBI Official Synonym Symbols
PP4; PPX; PP4C; PPH3; PPP4
UniProt Protein Name
Serine/threonine-protein phosphatase 4 catalytic subunit
UniProt Synonym Protein Names
Protein phosphatase X; PP-X
UniProt Gene Name
PPP4C
UniProt Synonym Gene Names
PPP4; PPX; PP4C; Pp4; PP-X
UniProt Entry Name
PP4C_HUMAN
NCBI Protein Information
serine/threonine-protein phosphatase 4 catalytic subunit; PP-X; protein phosphatase X, catalytic subunit; protein phosphatase 4 (formerly X), catalytic subunit

UniProt Comments for PPP4C

PPP4C: a Ser/Thr phosphatase. A positive regulator of HPK1 and the HPK1-JNK signaling pathway. Mediates TNF-alpha-induced degradation of IRS-4 and plays a role in NF-kappaB p65 Thr dephosphorylation. Holoenzyme consists at least of a catalytic subunit PPP4C and a regulatory subunit PPP4R1.

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; microtubule organizing center; nucleus

Molecular Function: NF-kappaB-inducing kinase activity; protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: dephosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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