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LL37 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242692

MBS242692 is an antibody targeting LL37. Also known as Rabbit Polyclonal to Human LL37 / CAMP / Cathelicidin. Reactive in Human. Host: LL37. More details

$750 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LL37 / CAMP / Cathelicidin, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human LL37 / CAMP / Cathelicidin
Product Gene Name
anti-LL37 antibody
Product Synonym Names
CAMP | Antibacterial protein fall-39 | CAP-18 | CRAMP | FALL-39 | FALL39 | HCAP-18 | HSD26 | LL37 | CAP18
Clonality
Polyclonal
Isotype
IgG
Host
LL37
Species Reactivity
Human
Form/Format
PBS containing 0.02% Sodium azide
Purity/Purification
Peptide column affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at 4°C for up to 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
OMIM
600474
3D Structure
ModBase 3D Structure for P49913
Host Note
Cathelicidin antibody was produced in Rabbit
Target
LL37 / Cathelicidin
Immunogen Species
LL37 Cathelicidin antibody was raised against Human
Antigen Type
Synthetic peptide
Immunogen
LL37 / Cathelicidin antibody was raised against a 17 AA synthetic peptide near the center of the human Cathelicidin. The immunogen is located within aa 50-100 of Cathelicidin.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-LL37 antibody
Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-LL37 antibody
IHC - Paraffin (5 ug/ml)
Western blot (1 ug/ml)
(Optimal dilution to be determined by the researcher)

Usage:
Cathelicidin antibody can be used for detection of Cathelicidin by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 19 kDa Observed: 18 kDa

Immunohistochemistry (IHC) of anti-LL37 antibody

Anti-CAMP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-LL37 Polyclonal Antibody – Cat# MBS242692

Immunofluorescence (IF) of anti-LL37 antibody

Immunofluorescence of Cathelicidin in Human Spleen cells with Cathelicidin antibody at 20 ug/ml.
Anti-LL37 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS242692

Western Blot (WB) of anti-LL37 antibody

Western blot of Cathelicidin in Human spleen tissue lysate with Cathelicidin antibody at 1 ug/ml.
Anti-LL37 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242692

Other Notes

Small volumes of anti-LL37 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LL37. It may not necessarily be applicable to this product.

NCBI GI #
39753970
NCBI GeneID
820
NCBI Accession #
NP_004336.2
UniProt Primary Accession #
P49913
UniProt Secondary Accession #
Q71SN9
UniProt Related Accession #
P49913
GenBank Nucleotide #
NM_004345.3
NCBI Official Full Name
cathelicidin antimicrobial peptide
NCBI Official Synonym Full Names
cathelicidin antimicrobial peptide
NCBI Official Symbol
CAMP
NCBI Official Synonym Symbols
LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39
UniProt Protein Name
Cathelicidin antimicrobial peptide
UniProt Synonym Protein Names
18 kDa cationic antimicrobial protein; CAP-18; hCAP-18
UniProt Gene Name
CAMP
UniProt Synonym Gene Names
CAP18; FALL39; CAP-18; hCAP-18
UniProt Entry Name
CAMP_HUMAN
NCBI Protein Information
cathelicidin antimicrobial peptide; 18 kDa cationic antimicrobial protein

NCBI Summary for LL37

This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. [provided by RefSeq, Sep 2014]
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UniProt Comments for LL37

CAMP: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. Belongs to the cathelicidin family.

Protein type: Cell cycle regulation; Vesicle; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: extracellular space; specific granule; cell projection; cell wall; cytosol

Molecular Function: protein binding

Biological Process: interaction with host; defense response to Gram-positive bacterium; positive regulation of angiogenesis; innate immune response in mucosa; killing by host of symbiont cells; positive regulation of cell proliferation; defense response to bacterium; antibacterial humoral response; positive regulation of protein amino acid phosphorylation; defense response to Gram-negative bacterium
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Research Articles on LL37

1. Competition assays, cross-linking experiments, limited proteolysis, and mass spectrometry revealed that LL-37 binds by specific hydrophobic interactions to the His-40-Lys-50 segment of actin, located in the DNase I binding loop.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LL37 antibody

 Reference Product  PubMed Publications
 EGFR antibody  >173 publications with LL37 and EGFR
 IL6 antibody  >66 publications with LL37 and IL6
 VDR antibody  >56 publications with LL37 and VDR
 IGF1R antibody  >50 publications with LL37 and IGF1R
 IL8 antibody  >26 publications with LL37 and IL8
 FPR2 antibody  >14 publications with LL37 and FPR2
 IL18 antibody  >12 publications with LL37 and IL18
 TLR9 antibody  >12 publications with LL37 and TLR9
 P2RX7 antibody  >4 publications with LL37 and P2RX7
 PRTN3 antibody  >1 publications with LL37 and PRTN3

Pathways associated with anti-LL37 antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway antibodies  Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway Diagram
 Phagosomal Maturation (early Endosomal Stage) Pathway antibodies  Phagosomal Maturation (early Endosomal Stage) Pathway Diagram
 SREBP Signalling Pathway antibodies  SREBP Signalling Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram

Diseases associated with anti-LL37 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3378 publications with LL37 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >2842 publications with LL37 and Cardiovascular Diseases
 Inflammation Antibodies  >1890 publications with LL37 and Inflammation
 Lung Diseases Antibodies  >1842 publications with LL37 and Lung Diseases
 Fibrosis Antibodies  >1686 publications with LL37 and Fibrosis
 Necrosis Antibodies  >1629 publications with LL37 and Necrosis
 Hypertension Antibodies  >1011 publications with LL37 and Hypertension
 Kidney Diseases Antibodies  >945 publications with LL37 and Kidney Diseases
 Neoplasms, Experimental Antibodies  >876 publications with LL37 and Neoplasms, Experimental
 Liver Diseases Antibodies  >834 publications with LL37 and Liver Diseases

Organs/Tissues associated with anti-LL37 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11526 publications with LL37 and Blood
 Heart Antibodies  >5426 publications with LL37 and Heart
 Thyroid Antibodies  >2271 publications with LL37 and Thyroid
 Testis Antibodies  >1554 publications with LL37 and Testis
 Pancreas Antibodies  >1523 publications with LL37 and Pancreas
 Eye Antibodies  >1419 publications with LL37 and Eye
 Bone Marrow Antibodies  >612 publications with LL37 and Bone Marrow
 Epididymis Antibodies  >179 publications with LL37 and Epididymis
 Lymph Node Antibodies  >176 publications with LL37 and Lymph Node
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