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SP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS242795

MBS242795 is an antibody targeting SP1. Also known as Rabbit Polyclonal (IgG) to Human SP1. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
SP1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human SP1
Product Gene Name
anti-SP1 antibody
Product Synonym Names
Anti-SP1 Antibody (aa421-470) IHC-plus; SP1; Sp1 transcription factor; Transcription factor Sp1; Specificity protein 1; TSFP1; Human SP1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
SP1 Antibody detects endogenous levels of total SP1 protein.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Molecular Weight
75,824 Da
OMIM
189906
3D Structure
ModBase 3D Structure for P08047
Target Species
Human
Immunogen Description
Synthetic peptide from human SP1 (aa421-470).
Immunogen
SP1 antibody was raised against synthetic peptide from human SP1 (aa421-470).
Antigen Modification
aa421-470

Product Categories/Family for anti-SP1 antibody

Family: Transcription factor
Applications Tested/Suitable for anti-SP1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SP1 antibody
ELISA (1:20000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-SP1 antibody

Anti-SP1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-SP1 Polyclonal Antibody – Cat# MBS242795

Immunofluorescence (IF) of anti-SP1 antibody

Immunofluorescence of A549 cells, using SP1 Antibody. The picture on the right is treated with the synthesized peptide.
Rabbit Anti-SP1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS242795

Western Blot (WB) of anti-SP1 antibody

Western blot of extracts from LOVO cells, using SP1 Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-SP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS242795

Other Notes

Small volumes of anti-SP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SP1. It may not necessarily be applicable to this product.

NCBI GI #
38372901
NCBI GeneID
6667
NCBI Accession #
NP_612482.2
UniProt Primary Accession #
P08047
UniProt Secondary Accession #
Q86TN8; Q9H3Q5; Q9NR51; Q9NY21; Q9NYE7; E4Z9M7; G5E9M8
UniProt Related Accession #
P08047
GenBank Nucleotide #
NM_138473.2
NCBI Official Full Name
transcription factor Sp1 isoform a
NCBI Official Synonym Full Names
Sp1 transcription factor
NCBI Official Symbol
SP1
UniProt Protein Name
Transcription factor Sp1
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
SP1
UniProt Synonym Gene Names
TSFP1
UniProt Entry Name
SP1_HUMAN
NCBI Protein Information
transcription factor Sp1; specificity protein 1

NCBI Summary for SP1

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
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UniProt Comments for SP1

SP1: a transcription factor of the Sp1 C2H2-type zinc-finger protein family. Phosphorylated and activated by MAPK. Dephosphorylation by PTEN inhibits DNA binding. Binds to p38 in the nucleus. Interacts with Huntingtin and TAFII130. Transcriptional activity of SP1 and TAFII130 disrupted in early Huntingtin's Disease.

Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein C-terminus binding; histone acetyltransferase binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; histone deacetylase binding; metal ion binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor binding; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; embryonic placenta development; ossification; transcription, DNA-dependent; viral reproduction; megakaryocyte differentiation; embryonic skeletal development; positive regulation of transcription, DNA-dependent; embryonic process involved in female pregnancy; rhythmic process; cellular lipid metabolic process; enucleate erythrocyte differentiation; liver development; trophectodermal cell differentiation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; lung development
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Research Articles on SP1

1. the mechanism of transcriptional activation of LTBP3 promoter depends on MALAT1 initiated from neighboring gene LTBP3 and involves both the direct interaction of the Sp1 and promoter-specific activation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SP1 antibody

 Reference Product  PubMed Publications
 SP3 antibody  >1238 publications with SP1 and SP3
 MYC antibody  >293 publications with SP1 and MYC
 HDAC1 antibody  >102 publications with SP1 and HDAC1
 E2F1 antibody  >89 publications with SP1 and E2F1
 TP53 antibody  >51 publications with SP1 and TP53
 SMAD2 antibody  >41 publications with SP1 and SMAD2
 SMAD4 antibody  >41 publications with SP1 and SMAD4
 HDAC2 antibody  >39 publications with SP1 and HDAC2
 EP300 antibody  >21 publications with SP1 and EP300
 ESR1 antibody  >17 publications with SP1 and ESR1

Pathways associated with anti-SP1 antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activation Of Gene Expression By SREBF (SREBP) Pathway antibodies  Activation Of Gene Expression By SREBF (SREBP) Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram

Diseases associated with anti-SP1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >344 publications with SP1 and Breast Neoplasms
 Nervous System Diseases Antibodies  >285 publications with SP1 and Nervous System Diseases
 Liver Diseases Antibodies  >269 publications with SP1 and Liver Diseases
 Necrosis Antibodies  >268 publications with SP1 and Necrosis
 Cardiovascular Diseases Antibodies  >230 publications with SP1 and Cardiovascular Diseases
 Inflammation Antibodies  >226 publications with SP1 and Inflammation
 Liver Neoplasms Antibodies  >205 publications with SP1 and Liver Neoplasms
 Disease Models, Animal Antibodies  >203 publications with SP1 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >186 publications with SP1 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >183 publications with SP1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-SP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >323 publications with SP1 and Bone
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