• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NUP50 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243043

MBS243043 is an antibody targeting NUP50. Also known as Goat Polyclonal to Human NUP50. Reactive in Gibbon, Bovine, Human, MousePredicted Reactivity: Monkey, Hamster, Pig, Chicken (at least 90% immunogen sequence identity). Host: Goat. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
NUP50, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human NUP50
Product Gene Name
anti-NUP50 antibody
Product Synonym Names
Anti-NUP50 Antibody (C-Terminus) IHC-plus; NUP50; 50 kDa nucleoporin; Nucleoporin 50kD; Nucleoporin 50kDa; NPAP60; NPAP60L; Nucleoporin Nup50; Human NUP50
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Bovine, Human, Mouse
Predicted Reactivity: Monkey, Hamster, Pig, Chicken (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human NUP50. This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1).
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
46,863 Da
OMIM
604646
3D Structure
ModBase 3D Structure for Q9UKX7
Target Species
Human
Immunogen Description
Synthetic peptide C-ELHKILLEKKDA from the C-terminus of human NUP50 (NP_009103.2; NP_705931.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Mouse, Bovine (100%); Marmoset, Hamster, Pig, Opossum, Turkey, Chicken, Platypus (92%).
Immunogen Type
Synthetic peptide
Immunogen
NUP50 antibody was raised against synthetic peptide C-ELHKILLEKKDA from the C-terminus of human NUP50 (NP_009103.2; NP_705931.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Mouse, Bovine (100%); Marmoset, Hamster, Pig, Opossum, Turkey, Chicken, Platypus (92%); Rat, Panda, Dog, Rabbit, Horse, Lizard, Xenopus (83%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-NUP50 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-NUP50 antibody
ELISA, IHC-P (2.5 - 3.75 ug/ml), WB (0.2 - 1 ug/ml)

Immunohistochemistry (IHC) of anti-NUP50 antibody

Anti-NUP50 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Goat Anti-NUP50 Polyclonal Antibody – Cat# MBS243043

Western Blot (WB) of anti-NUP50 antibody

Antibody staining (0.5 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Goat Anti-NUP50 Polyclonal Antibody, Western Blot (WB) – Cat# MBS243043

Other Notes

Small volumes of anti-NUP50 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NUP50. It may not necessarily be applicable to this product.

NCBI GI #
24497451
NCBI GeneID
10762
NCBI Accession #
NP_009103.2
UniProt Primary Accession #
Q9UKX7
UniProt Secondary Accession #
O75644; Q8N6V5; Q9NPM9; Q9NPR6; Q9P1K5; B1AHA4; B2RB15
UniProt Related Accession #
Q9UKX7
GenBank Nucleotide #
NM_007172.3
NCBI Official Full Name
nuclear pore complex protein Nup50 isoform b
NCBI Official Synonym Full Names
nucleoporin 50kDa
NCBI Official Symbol
NUP50
NCBI Official Synonym Symbols
NPAP60; NPAP60L
UniProt Protein Name
Nuclear pore complex protein Nup50
UniProt Synonym Protein Names
50 kDa nucleoporin; Nuclear pore-associated protein 60 kDa-like; Nucleoporin Nup50
Protein Family
Nuclear pore complex protein
UniProt Gene Name
NUP50
UniProt Synonym Gene Names
NPAP60L
UniProt Entry Name
NUP50_HUMAN
NCBI Protein Information
nuclear pore complex protein Nup50; nucleoporin 50kD; nucleoporin Nup50; 50 kDa nucleoporin; nuclear pore-associated protein 60L

NCBI Summary for NUP50

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for NUP50

NUP50: Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin- alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleoporin; Nuclear import

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nuclear pore

Molecular Function: protein binding

Biological Process: mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; viral infectious cycle; glucose transport; protein transport; intracellular transport; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport
Show more

Research Articles on NUP50

1. analysis of the Nup153-Nup50 protein interface and its role in nuclear import

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NUP50 antibody

 Reference Product  PubMed Publications
 NUP153 antibody  >8 publications with NUP50 and NUP153
 NUP93 antibody  >3 publications with NUP50 and NUP93
 RAN antibody  >3 publications with NUP50 and RAN
 NUP107 antibody  >2 publications with NUP50 and NUP107
 KPNB1 antibody  >1 publications with NUP50 and KPNB1
 NUP62 antibody  >1 publications with NUP50 and NUP62
 KPNA1 antibody  >1 publications with NUP50 and KPNA1

Pathways associated with anti-NUP50 antibody

 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Export Of Viral Ribonucleoproteins From Nucleus Pathway antibodies  Export Of Viral Ribonucleoproteins From Nucleus Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Glucose Transport Pathway antibodies  Glucose Transport Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram

Diseases associated with anti-NUP50 antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >3 publications with NUP50 and Death
 Nervous System Diseases Antibodies  >1 publications with NUP50 and Nervous System Diseases
 Liver Diseases Antibodies  >1 publications with NUP50 and Liver Diseases
 Fetal Death Antibodies  >1 publications with NUP50 and Fetal Death

Organs/Tissues associated with anti-NUP50 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3 publications with NUP50 and Liver
 Eye Antibodies  >1 publications with NUP50 and Eye
 Testis Antibodies  >1 publications with NUP50 and Testis
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000