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AKT3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243055

AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including… More details

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Product Overview

Product Name
AKT3, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human AKT3
Product Gene Name
Product Synonym Names
Anti-AKT3 Antibody (Internal) IHC-plus; AKT3; AKT3/PKBgamma; PRKBG; PKB gamma; Protein kinase Akt-3; RAC-gamma; MPPH; PKB-GAMMA; PKBG; Protein kinase B gamma; RAC-PK-gamma; STK-2; Human AKT3
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Predicted Reactivity: Hamster, Zebrafish (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human AKT3. This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1; NP_001193658.1).
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
54,032 Da
OMIM
gene 615937
3D Structure
ModBase 3D Structure for Q9Y243
Target Species
Human
Immunogen Description
Synthetic peptide CSPTSQIDNIGEEEM from an internal region of human AKT3 (NP_005456.1; NP_859029.1).
Immunogen Type
Synthetic peptide
Immunogen
AKT3 antibody was raised against synthetic peptide CSPTSQIDNIGEEEM from an internal region of human AKT3 (NP_005456.1; NP_859029.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Hamster, Zebrafish (93%); Stickleback (80%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-AKT3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-AKT3 antibody
ELISA (1:16000), IHC-P (5 ug/ml), WB (1 - 3 ug/ml)

Immunohistochemistry (IHC) of anti-AKT3 antibody

Anti-AKT3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-AKT3 Polyclonal Antibody – Cat# MBS243055

Immunohistochemistry (IHC) of anti-AKT3 antibody

Anti-AKT3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-AKT3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS243055

Western Blot (WB) of anti-AKT3 antibody

Staining (1 ug/ml) of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-AKT3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS243055

Other Notes

Small volumes of anti-AKT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AKT3 purchase

MBS243055 is an antibody targeting AKT3. Also known as Goat Polyclonal to Human AKT3. Reactive in Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Predicted Reactivity: Hamster, Zebrafish (at least 90% immunogen sequence identity). Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for AKT3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RAC-gamma serine/threonine-protein kinase isoform 1
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 3
NCBI Official Symbol
AKT3
NCBI Official Synonym Symbols
MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
UniProt Protein Name
RAC-gamma serine/threonine-protein kinase
UniProt Synonym Protein Names
Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2
UniProt Gene Name
AKT3
UniProt Synonym Gene Names
PKBG; PKB gamma
UniProt Entry Name
AKT3_HUMAN
NCBI Protein Information
RAC-gamma serine/threonine-protein kinase; PKB gamma; RAC-gamma serine/threonine protein kinase; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)

NCBI Summary for AKT3

The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for AKT3

Akt3: an AGC kinase that plays a critical role in controlling the balance between survival and apoptosis. Stimulated by platelet-derived growth factor, insulin, and insulin-like growth factor 1. Alternatively spliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; AGC group; AKT family

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: mitochondrial genome maintenance; signal transduction; protein amino acid phosphorylation

Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 2
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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