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MSI1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243096

MBS243096 is an antibody targeting MSI1. Also known as Rabbit Polyclonal to Human MSI1 / Musashi 1. Reactive in Dog, Horse, Human, Monkey, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
MSI1 / Musashi 1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human MSI1 / Musashi 1
Product Gene Name
Product Synonym Names
Anti-MSI1 / Musashi 1 Antibody (aa200-250) IHC-plus; MSI1; Musashi homolog 1 (Drosophila); Musashi RNA-binding protein 1; Musashi (Drosophila) homolog 1; Musashi1; Musashi 1; Musashi-1; Human MSI1; Musashi 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Dog, Horse, Human, Monkey, Mouse, Rat
Form/Format
PBS, 0.05% BSA, 0.05% sodium azide
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human MSI1
Preparation and Storage
Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.
Molecular Weight
39,125 Da
OMIM
603328
3D Structure
ModBase 3D Structure for O43347
Target Species
Human
Immunogen Description
A portion of amino acids 200-250 of human Musashi-1 was used as immunogen.
Immunogen Type
Synthetic peptide
Immunogen
MSI1 / Musashi 1 antibody was raised against a portion of amino acids 200-250 of human Musashi-1 was used as immunogen.
Antigen Modification
aa200-250
Applications Tested/Suitable for anti-MSI1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-MSI1 antibody
IHC-P (10 ug/ml), WB (2 - 4 ug/ml)

Immunohistochemistry (IHC) of anti-MSI1 antibody

Anti-MSI1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-MSI1 Polyclonal Antibody – Cat# MBS243096

Immunohistochemistry (IHC) of anti-MSI1 antibody

Anti-MSI1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-MSI1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS243096

Western Blot (WB) of anti-MSI1 antibody

Western blot of Musashi-1 in human small intestine lysate in the 1) absence and2) presence of immunizing peptide, 3) mouse small intestine, 4) rat small intestine and 5) mouse embryo brain lysate using at 2, 2, 4 and2 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
Rabbit Anti-MSI1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS243096

Other Notes

Small volumes of anti-MSI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MSI1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RNA-binding protein Musashi homolog 1
NCBI Official Synonym Full Names
musashi RNA-binding protein 1
NCBI Official Symbol
MSI1
UniProt Protein Name
RNA-binding protein Musashi homolog 1
UniProt Gene Name
MSI1
UniProt Synonym Gene Names
Musashi-1
UniProt Entry Name
MSI1H_HUMAN
NCBI Protein Information
RNA-binding protein Musashi homolog 1; musashi1; musashi-1

NCBI Summary for MSI1

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq, Jul 2008]
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UniProt Comments for MSI1

Musashi-1: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. Belongs to the Musashi family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: polysome; cytoplasm; nucleus

Molecular Function: RNA binding; nucleotide binding; poly(U) binding

Biological Process: nervous system development; epithelial cell differentiation; response to hormone stimulus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MSI1 antibody

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