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AKAP8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243273

MBS243273 is an antibody targeting AKAP8. Also known as Rabbit Polyclonal (IgG) to Human AKAP8 / AKAP95. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
AKAP8 / AKAP95, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human AKAP8 / AKAP95
Product Gene Name
Product Synonym Gene Name
AKAP95
Product Synonym Names
Anti-AKAP8 / AKAP95 Antibody (aa331-380) IHC-plus; AKAP8; A-kinase anchor protein 8; A-kinase anchor protein; 95kDa; A-kinase anchor protein 95 kDa; AKAP-8; AKAP95; AKAP 95; AKAP-95; Human AKAP8; AKAP95
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
AKAP8 Antibody detects endogenous levels of total AKAP8 protein.
Preparation and Storage
Store at -20 degree C for up to one year.
Molecular Weight
76,108 Da
OMIM
604692
3D Structure
ModBase 3D Structure for O43823
Target Species
Human
Immunogen Description
Synthetic peptide from human AKAP8 (aa331-380).
Immunogen
AKAP8 / AKAP95 antibody was raised against synthetic peptide from human AKAP8 (aa331-380).
Antigen Modification
aa331-380
Applications Tested/Suitable for anti-AKAP8 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-AKAP8 antibody
ELISA (1:20000), IF (1:100 - 1:500), IHC-P (5 ug/ml), WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-AKAP8 antibody

Anti-AKAP8 / AKAP95 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Rabbit Anti-AKAP8 Polyclonal Antibody – Cat# MBS243273

Immunofluorescence (IF) of anti-AKAP8 antibody

Immunofluorescence of HUVEC cells, using AKAP8 Antibody. The picture on the right is treated with the synthesized peptide.
Rabbit Anti-AKAP8 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS243273

Western Blot (WB) of anti-AKAP8 antibody

Western blot of extracts from mouse heart/Jurkat cells, using AKAP8 Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-AKAP8 Polyclonal Antibody, Western Blot (WB) – Cat# MBS243273

Other Notes

Small volumes of anti-AKAP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AKAP8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
A-kinase anchor protein 8
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein 8
NCBI Official Symbol
AKAP8
NCBI Official Synonym Symbols
AKAP-8; AKAP95; AKAP 95; AKAP-95
UniProt Protein Name
A-kinase anchor protein 8
UniProt Synonym Protein Names
A-kinase anchor protein 95 kDa; AKAP 95
UniProt Gene Name
AKAP8
UniProt Synonym Gene Names
AKAP95; AKAP-8; AKAP 95
UniProt Entry Name
AKAP8_HUMAN
NCBI Protein Information
A-kinase anchor protein 8; A-kinase anchor protein, 95kDa

NCBI Summary for AKAP8

This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]
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UniProt Comments for AKAP8

AKAP8: Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Belongs to the AKAP95 family.

Protein type: RNA-binding; Cell cycle regulation; Adaptor/scaffold; Mitochondrial

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; Golgi apparatus; nuclear matrix; membrane; mitochondrion; female pronucleus; condensed chromosome; nucleus

Molecular Function: zinc ion binding; double-stranded DNA binding

Biological Process: mitosis; mitotic chromosome condensation; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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