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UBE2F Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243297

MBS243297 is an antibody targeting UBE2F. Also known as Goat Polyclonal to Human UBE2F. Reactive in Gibbon, Gorilla, Human, Monkey, MousePredicted Reactivity: Bat, Bovine, Dog, Horse, Chicken, Zebrafish (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
UBE2F, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human UBE2F
Product Gene Name
Product Synonym Names
Anti-UBE2F Antibody (C-Terminus) IHC-plus; UBE2F; NCE2; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; NEDD8-conjugating enzyme UBE2F; Human UBE2F
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Gorilla, Human, Monkey, Mouse
Predicted Reactivity: Bat, Bovine, Dog, Horse, Chicken, Zebrafish (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human UBE2F / UBE2F.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
18,158 Da
3D Structure
ModBase 3D Structure for Q969M7
Target Species
Human
Immunogen Description
Synthetic peptide C-HLRDKEDFRNKVDD from the C-terminus of human UBE2F / UBE2F (NP_542409.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%).
Immunogen Type
Synthetic peptide
Immunogen
UBE2F antibody was raised against synthetic peptide C-HLRDKEDFRNKVDD from the C-terminus of human UBE2F / UBE2F (NP_542409.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken, Lizard, Salmon, Zebrafish (93%); Mouse, Rat, Hamster, Elephant, Rabbit, Platypus (86%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-UBE2F antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-UBE2F antibody
ELISA, IHC-P (2.5 - 3.75 ug/ml), WB (0.05 - 0.2 ug/ml)
Usage: Western Blot: Approx. 20kD band observed in Mouse Liver lysates (calculated MW of 21.1kD according to Human NP_542409.1 and to Mouse NP_080730.1). Recommended concentration: 0.05-0.2 ug/ml.

Immunohistochemistry (IHC) of anti-UBE2F antibody

Anti-UBE2F antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Goat Anti-UBE2F Polyclonal Antibody – Cat# MBS243297

Western Blot (WB) of anti-UBE2F antibody

UBE2F antibody (0.05 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-UBE2F Polyclonal Antibody, Western Blot (WB) – Cat# MBS243297

Other Notes

Small volumes of anti-UBE2F antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE2F. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NEDD8-conjugating enzyme UBE2F isoform 1
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2F (putative)
NCBI Official Symbol
UBE2F
NCBI Official Synonym Symbols
NCE2
UniProt Protein Name
NEDD8-conjugating enzyme UBE2F
UniProt Synonym Protein Names
NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; Ubiquitin-conjugating enzyme E2 F
UniProt Gene Name
UBE2F
UniProt Synonym Gene Names
NCE2
UniProt Entry Name
UBE2F_HUMAN
NCBI Protein Information
NEDD8-conjugating enzyme UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; NEDD8 carrier protein UBE2F

UniProt Comments for UBE2F

UBE2F: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm

Molecular Function: protein binding; ubiquitin protein ligase binding; ATP binding; ligase activity; NEDD8 ligase activity

Biological Process: protein neddylation; protein ubiquitination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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