Goat Anti-LIMS2 Polyclonal Antibody – Cat# MBS243325

Anti-LIMS2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

LIMS2 Antibody – Goat Polyclonal

Name: LIMS2 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS243325
Price: 625 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243325

MBS243325 is an antibody targeting LIMS2. Also known as Goat Polyclonal to Human LIMS2. Reactive in HumanPredicted Reactivity: Monkey (at least 90% immunogen sequence identity). Host: Goat. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

LIMS2, Polyclonal Antibody

Full Product Name

Goat Polyclonal to Human LIMS2

Product Gene Name

anti-LIMS2 antibody

Product Synonym Names

Anti-LIMS2 Antibody (Internal) IHC-plus; LIMS2; PINCH2; PINCH-2; LIM-like protein 2; ILK-binding protein; Human LIMS2

Clonality

Polyclonal

Host

Goat

Species Reactivity

Human
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)

Form/Format

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

Purity/Purification

Immunoaffinity Purified

Concentration

0.5 mg/ml (lot specific)

Specificity

Human LIMS2. This antibody is expected to recognize isoform 1 (NP_001129509.2) and isoformn 3 (NP_001154875.1).

Preparation and Storage

Store at -20 degree C. Minimize freezing and thawing.

Molecular Weight

41,226 Da

OMIM

607908

3D Structure

ModBase 3D Structure for Q7Z4I7

Target Species

Human

Immunogen Description

Synthetic peptide C-RWSNMSDALA from an internal region of human LIMS2 (NP_001129509.2; NP_001154875.1). Percent identity with other species by BLAST analysis: Human (100%), Marmoset (90%), Gorilla (80%), Monkey (80%), Rabbit (80%), Opossum (80%).

Immunogen Type

Synthetic peptide

Immunogen

LIMS2 antibody was raised against synthetic peptide C-RWSNMSDALA from an internal region of human LIMS2 (NP_001129509.2; NP_001154875.1). Percent identity with other species by BLAST analysis: Human (100%), Marmoset (90%), Gorilla (80%), Monkey (80%), Rabbit (80%), Opossum (80%), Turkey (80%), Chicken (80%), Platypus (80%), Nectri (80%).

Antigen Modification

Internal

Applications Tested/Suitable for anti-LIMS2 antibody

Immunohistochemistry (IHC - Paraffin), ELISA (EIA)

Application Notes for anti-LIMS2 antibody

ELISA (1:8000), IHC-P (3.75 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Preliminary experiments gave an approx 80kD band in Human Heart and Mouse Heart lysates after 0.5 g/ml antibody staining. Please note that currently we cannot find an explanation...

Other Notes

Small volumes of anti-LIMS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LIMS2. It may not necessarily be applicable to this product.

NCBI GI #

40804748

NCBI GeneID

55679

NCBI Accession #

NP_060450.2

UniProt Primary Accession #

Q7Z4I7

UniProt Secondary Accession #

Q7Z4I2; Q7Z4I6; Q7Z4I8; Q8NFE7; Q9HA13; A6NLH0; B4DMV1; F5H6E6

UniProt Related Accession #

Q7Z4I7

GenBank Nucleotide #

NM_017980.4

NCBI Official Full Name

LIM and senescent cell antigen-like-containing domain protein 2 isoform 2

NCBI Official Synonym Full Names

LIM and senescent cell antigen-like domains 2

NCBI Official Symbol

LIMS2

NCBI Official Synonym Symbols

PINCH2; PINCH-2

UniProt Protein Name

LIM and senescent cell antigen-like-containing domain protein 2

UniProt Synonym Protein Names

LIM-like protein 2; Particularly interesting new Cys-His protein 2; PINCH-2

Protein Family

LIM and senescent cell antigen-like-containing domain protein

UniProt Gene Name

LIMS2

UniProt Synonym Gene Names

PINCH2; PINCH-2

UniProt Entry Name

LIMS2_HUMAN

NCBI Protein Information

LIM and senescent cell antigen-like-containing domain protein 2; LIM-like protein 2; ILK-binding protein; particularly interesting new Cys-His protein 2

NCBI Summary for LIMS2

This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase), a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains, each domain forming two zinc fingers, which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

UniProt Comments for LIMS2

LIMS2: Competes with LIMS1 for binding to ILK. Plays a role in modulating cell spreading and migration. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q14.3

Cellular Component: focal adhesion; plasma membrane; nucleus; cytosol

Molecular Function: zinc ion binding

Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; negative regulation of apoptosis

Research Articles on LIMS2

1. PINCH-2 mRNA is overexpressed in malignant mesothelioma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LIMS2 antibody

Reference Product	PubMed Publications
ILK antibody	>8 publications with LIMS2 and ILK
PARVA antibody	>2 publications with LIMS2 and PARVA
PARVB antibody	>1 publications with LIMS2 and PARVB

Pathways associated with anti-LIMS2 antibody

Products by Pathway	Pathway Diagram
Cell Junction Organization Pathway antibodies	Cell Junction Organization Pathway Diagram
Cell-Cell Communication Pathway antibodies	Cell-Cell Communication Pathway Diagram
Cell-extracellular Matrix Interactions Pathway antibodies	Cell-extracellular Matrix Interactions Pathway Diagram

Diseases associated with anti-LIMS2 antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>2 publications with LIMS2 and Carcinoma
Cardiomegaly Antibodies	>1 publications with LIMS2 and Cardiomegaly
Congenital Abnormalities Antibodies	>1 publications with LIMS2 and Congenital Abnormalities
Disease Models, Animal Antibodies	>1 publications with LIMS2 and Disease Models, Animal
Cardiomyopathies Antibodies	>1 publications with LIMS2 and Cardiomyopathies
Fibrosis Antibodies	>1 publications with LIMS2 and Fibrosis
Liver Diseases Antibodies	>1 publications with LIMS2 and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>1 publications with LIMS2 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>1 publications with LIMS2 and Liver Neoplasms

Organs/Tissues associated with anti-LIMS2 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>3 publications with LIMS2 and Muscle
Embryonic Tissue Antibodies	>3 publications with LIMS2 and Embryonic Tissue
Kidney Antibodies	>2 publications with LIMS2 and Kidney
Stomach Antibodies	>2 publications with LIMS2 and Stomach
Heart Antibodies	>2 publications with LIMS2 and Heart
Liver Antibodies	>1 publications with LIMS2 and Liver
Testis Antibodies	>1 publications with LIMS2 and Testis
Blood Antibodies	>1 publications with LIMS2 and Blood
Uterus Antibodies	>1 publications with LIMS2 and Uterus