• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ZBED2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243388

MBS243388 is an antibody targeting ZBED2. Also known as Rabbit Polyclonal to Human ZBED2. Reactive in Human. Host: Rabbit. More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ZBED2, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human ZBED2
Product Gene Name
anti-ZBED2 antibody
Product Synonym Names
Anti-ZBED2 Antibody (N-Terminus) IHC-plus; ZBED2; Human ZBED2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Peptide Column Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED3.
Preparation and Storage
Store at 4°C for up 3 months. Store at -20°C for 1 year. Avoid freeze/thaw cycles.
OMIM
615246
3D Structure
ModBase 3D Structure for Q9BTP6
Immunogen
ZBED2 antibody was raised against a 15 AA synthetic peptide near the N-Terminus of human ZBED2. The immunogen is located within the first 50 AA of ZBED2.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-ZBED2 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA)
Application Notes for anti-ZBED2 antibody
ELISA, ICC, IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: ZBED2 antibody can be used for detection of ZBED2 by Western blot at 1-2 ug/ml.

Immunohistochemistry (IHC) of anti-ZBED2 antibody

Anti-ZBED2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-ZBED2 Polyclonal Antibody – Cat# MBS243388

Immunocytochemistry (ICC) of anti-ZBED2 antibody

Immunocytochemistry of ZBED2 in A549 cells with ZBED2 antibody at 5 ug/ml.
Rabbit Anti-ZBED2 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS243388

Western Blot (WB) of anti-ZBED2 antibody

Western blot of ZBED2 in A549 cell lysate with ZBED2 antibody at (A) 1 and (B) 2 ug/ml.
Rabbit Anti-ZBED2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS243388

Other Notes

Small volumes of anti-ZBED2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ZBED2. It may not necessarily be applicable to this product.

NCBI GI #
56790947
NCBI GeneID
79413
NCBI Accession #
NP_078784.2
UniProt Primary Accession #
Q9BTP6
UniProt Secondary Accession #
D3DN62
UniProt Related Accession #
Q9BTP6
GenBank Nucleotide #
NM_024508.4
NCBI Official Full Name
zinc finger BED domain-containing protein 2
NCBI Official Synonym Full Names
zinc finger, BED-type containing 2
NCBI Official Symbol
ZBED2
UniProt Protein Name
Zinc finger BED domain-containing protein 2
Protein Family
Zinc finger BED domain-containing protein
UniProt Gene Name
ZBED2
UniProt Entry Name
ZBED2_HUMAN
NCBI Protein Information
zinc finger BED domain-containing protein 2; zinc finger, BED domain containing 2

UniProt Comments for ZBED2

ZBED2:

Protein type: C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 3q13.2

Molecular Function: DNA binding; metal ion binding

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000