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DOK3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243418

MBS243418 is an antibody targeting DOK3. Also known as Goat Polyclonal to Human DOK3. Reactive in HumanPredicted Reactivity: Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Rabbit (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DOK3, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human DOK3
Product Gene Name
anti-DOK3 antibody
Product Synonym Names
Anti-DOK3 Antibody (C-Terminus) IHC-plus; DOK3; Docking protein 3; Dok-like protein; DOKL; Human DOK3
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Predicted Reactivity: Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Rabbit (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human DOK3.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
24,737 Da
OMIM
611435
3D Structure
ModBase 3D Structure for Q7L591
Target Species
Human
Immunogen Description
Synthetic peptide TRERRKGPAPCDRP from the C-terminus of human DOK3 (NP_079148.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Rabbit (93%); Bovine, Bat (86%).
Immunogen Type
Synthetic peptide
Immunogen
DOK3 antibody was raised against synthetic peptide TRERRKGPAPCDRP from the C-terminus of human DOK3 (NP_079148.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Rabbit (93%); Bovine, Bat (86%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-DOK3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-DOK3 antibody
ELISA (1:64000), IHC-P (6.12 ug/ml), WB (0.1 - 0.3 ug/ml)

Immunohistochemistry (IHC) of anti-DOK3 antibody

Anti-DOK3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6.125 ug/ml.
Goat Anti-DOK3 Polyclonal Antibody – Cat# MBS243418

Immunohistochemistry (IHC) of anti-DOK3 antibody

Anti-DOK3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6.125 ug/ml.
Goat Anti-DOK3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS243418

Western Blot (WB) of anti-DOK3 antibody

Antibody staining (0.1 ug/ml) of Human PBMC lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Goat Anti-DOK3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS243418

Other Notes

Small volumes of anti-DOK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DOK3. It may not necessarily be applicable to this product.

NCBI GI #
221554537
NCBI GeneID
79930
NCBI Accession #
NP_079148.2
UniProt Primary Accession #
Q7L591
UniProt Secondary Accession #
Q8N864; Q9BQB3; Q9H666; E9PAT0; H7BXS0
UniProt Related Accession #
Q7L591
GenBank Nucleotide #
NM_024872.2
NCBI Official Full Name
docking protein 3 isoform 1
NCBI Official Synonym Full Names
docking protein 3
NCBI Official Symbol
DOK3
NCBI Official Synonym Symbols
DOKL
UniProt Protein Name
Docking protein 3
UniProt Synonym Protein Names
Downstream of tyrosine kinase 3
Protein Family
Docking protein
UniProt Gene Name
DOK3
UniProt Entry Name
DOK3_HUMAN
NCBI Protein Information
docking protein 3; Dok-like protein; downstream of tyrosine kinase 3

UniProt Comments for DOK3

DOK3: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with SHIP. May modulate Abl function. Upon tyrosine phosphorylation, Dok3 interacts with CSK and SHIP via their SH2 domains. Both pY381 and pY398 are required for interaction with SHIP. Only pY381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Does not interact with RasGAP. Four isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: cytoplasm; plasma membrane

Molecular Function: protein binding; insulin receptor binding

Biological Process: Ras protein signal transduction
Show more

Research Articles on DOK3

1. The Dok-3/Grb2 protein signal module attenuates Lyn kinase-dependent activation of Syk kinase in B cell antigen receptor microclusters

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DOK3 antibody

 Reference Product  PubMed Publications
 SRC antibody  >9 publications with DOK3 and SRC
 GRB2 antibody  >7 publications with DOK3 and GRB2
 LYN antibody  >2 publications with DOK3 and LYN
 CSK antibody  >2 publications with DOK3 and CSK

Pathways associated with anti-DOK3 antibody

 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram

Diseases associated with anti-DOK3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2 publications with DOK3 and Inflammation
 Hypertension Antibodies  >1 publications with DOK3 and Hypertension
 Adenocarcinoma Antibodies  >1 publications with DOK3 and Adenocarcinoma
 Necrosis Antibodies  >1 publications with DOK3 and Necrosis
 Hyperplasia Antibodies  >1 publications with DOK3 and Hyperplasia
 Lung Neoplasms Antibodies  >1 publications with DOK3 and Lung Neoplasms

Organs/Tissues associated with anti-DOK3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11 publications with DOK3 and Blood
 Lung Antibodies  >3 publications with DOK3 and Lung
 Bone Marrow Antibodies  >2 publications with DOK3 and Bone Marrow
 Heart Antibodies  >1 publications with DOK3 and Heart
 Thymus Antibodies  >1 publications with DOK3 and Thymus
 Kidney Antibodies  >1 publications with DOK3 and Kidney
 Lymph Node Antibodies  >1 publications with DOK3 and Lymph Node
 Spleen Antibodies  >1 publications with DOK3 and Spleen
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