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PUMA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243445

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53… More details

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Product Overview

Product Name
PUMA, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human PUMA
Product Gene Name
anti-PUMA antibody
Product Synonym Gene Name
BBC3
Product Synonym Names
Anti-PUMA Antibody (N-Terminus) IHC-plus; BBC3; Bcl-2-binding component 3; BCL2 binding component 3; JFY-1; JFY1; PUMA; Human PUMA
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Gibbon, Human, Mouse, Rat
Form/Format
PBS, 0.02% sodium azide.
Purity/Purification
Purified
Concentration
1 mg/ml (lot specific)
Specificity
Recognizes the p53 - upregulated modulator of apoptosis (PUMA), also known as bbc3. PUMA is a pro-apoptotic member of the BCL-2 family. PUMA is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
10,326 Da
OMIM
605854
3D Structure
ModBase 3D Structure for Q9BXH1
Target Species
Human
Immunogen Description
Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human PUMA alpha. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%); Opossum (87%).
Immunogen Type
Synthetic peptide
Immunogen
PUMA antibody was raised against synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human PUMA alpha. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%); Opossum (87%).
Antigen Modification
N-Terminus

Product Categories/Family for anti-PUMA antibody

Family: Apoptosis
Applications Tested/Suitable for anti-PUMA antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-PUMA antibody
IHC-P (2.5 ug/ml), WB (2 - 4 ug/ml)
Usage: Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Histology Positive Control Tissue: Human breast carcinoma. Western...

Immunohistochemistry (IHC) of anti-PUMA antibody

Anti-BBC3 / PUMA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Rabbit Anti-PUMA Polyclonal Antibody – Cat# MBS243445

Other Notes

Small volumes of anti-PUMA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PUMA purchase

MBS243445 is an antibody targeting PUMA. Also known as Rabbit Polyclonal (IgG) to Human PUMA. Reactive in Gibbon, Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PUMA. It may not necessarily be applicable to this product.

NCBI GI #
15193488
NCBI GeneID
27113
NCBI Accession #
NP_055232.1
UniProt Primary Accession #
Q9BXH1
UniProt Secondary Accession #
O00171; Q96PG9; B9EGI3
UniProt Related Accession #
Q96PG8; Q9BXH1
GenBank Nucleotide #
NM_014417.4
NCBI Official Full Name
bcl-2-binding component 3 isoform 4
NCBI Official Synonym Full Names
BCL2 binding component 3
NCBI Official Symbol
BBC3
NCBI Official Synonym Symbols
JFY1; PUMA; JFY-1
UniProt Protein Name
Bcl-2-binding component 3
UniProt Synonym Protein Names
JFY-1; p53 up-regulated modulator of apoptosis
Protein Family
Hydroxycarboxylic acid receptor
UniProt Gene Name
BBC3
UniProt Synonym Gene Names
PUMA
UniProt Entry Name
BBC3_HUMAN
NCBI Protein Information
bcl-2-binding component 3; p53 up-regulated modulator of apoptosis

NCBI Summary for PUMA

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
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UniProt Comments for PUMA

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth
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Research Articles on PUMA

1. c-Jun transactivates Puma gene expression to promote osteoarthritis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PUMA antibody

 Reference Product  PubMed Publications
 PMAIP1 antibody  >72 publications with PUMA and PMAIP1
 TP53 antibody  >58 publications with PUMA and TP53
 BCL2L1 antibody  >46 publications with PUMA and BCL2L1
 BID antibody  >26 publications with PUMA and BID
 BAD antibody  >24 publications with PUMA and BAD
 MCL1 antibody  >17 publications with PUMA and MCL1
 SP1 antibody  >3 publications with PUMA and SP1
 SNAI2 antibody  >3 publications with PUMA and SNAI2
 BCL2L2 antibody  >3 publications with PUMA and BCL2L2

Pathways associated with anti-PUMA antibody

 Products by Pathway  Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of PUMA And Translocation To Mitochondria Pathway antibodies  Activation Of PUMA And Translocation To Mitochondria Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway antibodies  BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway Diagram
 DNA Damage Response Pathway antibodies  DNA Damage Response Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram

Diseases associated with anti-PUMA antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >73 publications with PUMA and Carcinoma
 Adenocarcinoma Antibodies  >33 publications with PUMA and Adenocarcinoma
 Breast Neoplasms Antibodies  >31 publications with PUMA and Breast Neoplasms
 Lung Neoplasms Antibodies  >24 publications with PUMA and Lung Neoplasms
 Necrosis Antibodies  >20 publications with PUMA and Necrosis
 Liver Diseases Antibodies  >18 publications with PUMA and Liver Diseases
 Lung Diseases Antibodies  >17 publications with PUMA and Lung Diseases
 Nervous System Diseases Antibodies  >16 publications with PUMA and Nervous System Diseases
 Carcinoma, Squamous Cell Antibodies  >13 publications with PUMA and Carcinoma, Squamous Cell
 Brain Diseases Antibodies  >13 publications with PUMA and Brain Diseases

Organs/Tissues associated with anti-PUMA antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >30 publications with PUMA and Lung
 Brain Antibodies  >18 publications with PUMA and Brain
 Kidney Antibodies  >13 publications with PUMA and Kidney
 Skin Antibodies  >12 publications with PUMA and Skin
 Prostate Antibodies  >12 publications with PUMA and Prostate
 Stomach Antibodies  >12 publications with PUMA and Stomach
 Muscle Antibodies  >9 publications with PUMA and Muscle
 Bone Antibodies  >9 publications with PUMA and Bone
 Eye Antibodies  >7 publications with PUMA and Eye
 Intestine Antibodies  >6 publications with PUMA and Intestine
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