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LEO1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243475

MBS243475 is an antibody targeting LEO1. Also known as Rabbit Polyclonal to Human LEO1. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
LEO1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human LEO1
Product Gene Name
anti-LEO1 antibody
Product Synonym Names
Anti-LEO1 Antibody (phospho-Ser10) IHC-plus; LEO1; RDL; Human LEO1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity Purified
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Molecular Weight
68,004 Da
OMIM
610507
3D Structure
ModBase 3D Structure for Q8WVC0
Target Species
Human
Immunogen Description
KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human LEO1.
Immunogen Type
Synthetic phospho-peptide
Immunogen
LEO1 antibody was raised against kLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human LEO1.
Antigen Modification
pSer10
Applications Tested/Suitable for anti-LEO1 antibody
Immunohistochemistry (IHC - Paraffin), Dot Blot (DB)
Application Notes for anti-LEO1 antibody
DB (1:500), IHC-P (5 ug/ml)

Immunohistochemistry (IHC) of anti-LEO1 antibody

Anti-LEO1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-LEO1 Polyclonal Antibody – Cat# MBS243475

Dot Blot (DB) of anti-LEO1 antibody

Dot blot analysis of anti-Phospho-LEO1-pS10 Antibody on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug per ml.
Rabbit Anti-LEO1 Polyclonal Antibody, Dot Blot (DB) – Cat# MBS243475

Other Notes

Small volumes of anti-LEO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LEO1. It may not necessarily be applicable to this product.

NCBI GI #
20270337
NCBI GeneID
123169
NCBI Accession #
NP_620147.1
UniProt Primary Accession #
Q8WVC0
UniProt Secondary Accession #
Q96N99
UniProt Related Accession #
Q8WVC0
GenBank Nucleotide #
NM_138792.3
NCBI Official Full Name
RNA polymerase-associated protein LEO1 isoform 1
NCBI Official Synonym Full Names
Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
NCBI Official Symbol
LEO1
NCBI Official Synonym Symbols
RDL
UniProt Protein Name
RNA polymerase-associated protein LEO1
UniProt Synonym Protein Names
Replicative senescence down-regulated leo1-like protein
Protein Family
RNA polymerase-associated protein
UniProt Gene Name
LEO1
UniProt Synonym Gene Names
RDL
UniProt Entry Name
LEO1_HUMAN
NCBI Protein Information
RNA polymerase-associated protein LEO1; replicative senescence down-regulated leo1-like protein

NCBI Summary for LEO1

LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008]
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UniProt Comments for LEO1

LEO1: a component of the PAF1 multifunctional complex involved in initiation and elongation, histone methylation and RNA processing. The PAF1 complex interacts with POLR2A. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts. The PAF1 complex consists of at least of CDC73, PAF1, LEO1 and CTR9. Interacts with CDC73. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: nucleoplasm; nucleolus

Molecular Function: protein binding

Biological Process: histone monoubiquitination; Wnt receptor signaling pathway; negative regulation of myeloid cell differentiation; endodermal cell fate commitment; transcription, DNA-dependent; positive regulation of RNA elongation from RNA polymerase II promoter; stem cell maintenance; histone H2B ubiquitination; positive regulation of mRNA 3'-end processing; mRNA polyadenylation; positive regulation of transcription from RNA polymerase II promoter
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Research Articles on LEO1

1. PRL-3 and Leo1 levels were positively associated in AML patient samples.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LEO1 antibody

 Reference Product  PubMed Publications
 PAF1 antibody  >26 publications with LEO1 and PAF1
 CDC73 antibody  >21 publications with LEO1 and CDC73
 CTR9 antibody  >20 publications with LEO1 and CTR9
 RTF1 antibody  >18 publications with LEO1 and RTF1

Pathways associated with anti-LEO1 antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies  RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By WNT In Cancer Pathway antibodies  Signaling By WNT In Cancer Pathway Diagram
 Signaling By Wnt Pathway antibodies  Signaling By Wnt Pathway Diagram
 TCF Dependent Signaling In Response To WNT Pathway antibodies  TCF Dependent Signaling In Response To WNT Pathway Diagram
 XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway antibodies  XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway Diagram
 Formation Of The Beta-catenin:TCF Transactivating Complex Pathway antibodies  Formation Of The Beta-catenin:TCF Transactivating Complex Pathway Diagram
 Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway antibodies  Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway Diagram

Diseases associated with anti-LEO1 antibody

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >2 publications with LEO1 and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >1 publications with LEO1 and Breast Neoplasms
 Kidney Neoplasms Antibodies  >1 publications with LEO1 and Kidney Neoplasms

Organs/Tissues associated with anti-LEO1 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >3 publications with LEO1 and Heart
 Muscle Antibodies  >1 publications with LEO1 and Muscle
 Lung Antibodies  >1 publications with LEO1 and Lung
 Liver Antibodies  >1 publications with LEO1 and Liver
 Ovary Antibodies  >1 publications with LEO1 and Ovary
 Embryonic Tissue Antibodies  >1 publications with LEO1 and Embryonic Tissue
 Kidney Antibodies  >1 publications with LEO1 and Kidney
 Brain Antibodies  >1 publications with LEO1 and Brain
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