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SAR1A Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243750

MBS243750 is an antibody targeting SAR1A. Also known as Goat Polyclonal to Human SAR1A / SAR1. Reactive in Gibbon, Gorilla, Human, OrangutanPredicted Reactivity: Monkey, Mouse, Rat, Rabbit, Chicken (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
SAR1A / SAR1, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human SAR1A / SAR1
Product Gene Name
Product Synonym Gene Name
SAR1
Product Synonym Names
Anti-SAR1A / SAR1 Antibody (Internal) IHC-plus; SAR1A; GTP-binding protein SAR1a; Masra2; SAR1 homolog A (S. cerevisiae); SAR1a gene homolog 1; SAR1; COPII-associated small GTPase; SAR1 gene homolog A; SARA; SARA1; Human SAR1A; SAR1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Gorilla, Human, Orangutan
Predicted Reactivity: Monkey, Mouse, Rat, Rabbit, Chicken (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human SAR1A.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
17,500 Da
OMIM
607691
3D Structure
ModBase 3D Structure for Q9NR31
Target Species
Human
Immunogen Description
Synthetic peptide C-HSRLVESKVELNA from an internal region of human SAR1A (NP_064535.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset (100%); Monkey, Mouse, Rat, Elephant, Rabbit, Turkey, Chicken (92%).
Immunogen Type
Synthetic peptide
Immunogen
SAR1A / SAR1 antibody was raised against synthetic peptide C-HSRLVESKVELNA from an internal region of human SAR1A (NP_064535.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset (100%); Monkey, Mouse, Rat, Elephant, Rabbit, Turkey, Chicken (92%); Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (85%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-SAR1A antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SAR1A antibody
ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 23 kD band observed in lysates of NIH3T3 (calculated MW of 22.4 kD according to Human NP_064535.1 and to Mouse NP_033146.1). Recommended concentration: 0.1-0.3 ug/ml.

Immunohistochemistry (IHC) of anti-SAR1A antibody

Anti-SAR1A antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Goat Anti-SAR1A Polyclonal Antibody – Cat# MBS243750

Immunohistochemistry (IHC) of anti-SAR1A antibody

Anti-SAR1A antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Goat Anti-SAR1A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS243750

Western Blot (WB) of anti-SAR1A antibody

Antibody (0.1 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-SAR1A Polyclonal Antibody, Western Blot (WB) – Cat# MBS243750

Other Notes

Small volumes of anti-SAR1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SAR1A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
GTP-binding protein SAR1a
NCBI Official Synonym Full Names
secretion associated, Ras related GTPase 1A
NCBI Official Symbol
SAR1A
NCBI Official Synonym Symbols
SAR1; Sara; SARA1; masra2
UniProt Protein Name
GTP-binding protein SAR1a
UniProt Synonym Protein Names
COPII-associated small GTPase
Protein Family
UniProt Gene Name
SAR1A
UniProt Synonym Gene Names
SAR1; SARA; SARA1
UniProt Entry Name
SAR1A_HUMAN
NCBI Protein Information
GTP-binding protein SAR1a; SAR1 homolog A; SAR1 gene homolog A; SAR1a gene homolog 1; COPII-associated small GTPase

UniProt Comments for SAR1A

SAR1A: Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). Belongs to the small GTPase superfamily. SAR1 family.

Protein type: G protein; G protein, monomeric, SAR1; G protein, monomeric

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: Golgi apparatus; sarcoplasmic reticulum

Molecular Function: GTP binding

Biological Process: vesicle-mediated transport; intracellular protein transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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