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GPR65 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243866

GPR65 (TDAG8), a Lysophospholipid/Lysosphingolipid Receptor, is a molecular target for psychosine, a toxic lipid formed by the breakdown of galactosylceramide (cerebroside). A build-up of psychosine, caused by the lack of galactosylceramidase, results in the… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GPR65 / TDAG8, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human GPR65 / TDAG8
Product Gene Name
anti-GPR65 antibody
Product Synonym Gene Name
TDAG8
Product Synonym Names
GPR65 Antibody, G-protein coupled receptor 65 Antibody, Gpcr25 Antibody, HTDAG8 Antibody, TDAG8 Antibody, T-cell death-associated gene 8 Antibody, Psychosine receptor Antibody, G protein-coupled receptor 65 Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Predicted Reactivity: Gorilla, Gibbon (at least 90% immunogen sequence identity)
Form/Format
PBS, less than 0.1% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human GPR65. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except VCL (50%), CPS1 (39%).
Preparation and Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Molecular Weight
39,333 Da
OMIM
604620
3D Structure
ModBase 3D Structure for Q8IYL9
Immunogen Species
Human
Immunogen Description
Synthetic 18 amino acid peptide from 3rd extracellular domain of human GPR65. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Gibbon (94%).
Immunogen Type
Synthetic peptide
Immunogen
GPR65 / TDAG8 antibody was raised against synthetic 18 amino acid peptide from 3rd extracellular domain of human GPR65. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Gibbon (94%).
Antigen Modification
Extracellular Domain
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-GPR65 antibody

Family: GPCR; Subfamily: Lysophospholipid/Lysosphingolipid
Applications Tested/Suitable for anti-GPR65 antibody
Immunohistochemistry (IHC - Paraffin)
Application Notes for anti-GPR65 antibody
IHC-P (10 ug/ml)
Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC) of anti-GPR65 antibody

Anti-GPR65 antibody IHC of human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-GPR65 Polyclonal Antibody – Cat# MBS243866

Other Notes

Small volumes of anti-GPR65 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPR65 purchase

MBS243866 is an antibody targeting GPR65. Also known as Rabbit Polyclonal to Human GPR65 / TDAG8. Reactive in Human
Predicted Reactivity: Gorilla, Gibbon (at least 90% immunogen sequence identity). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GPR65. It may not necessarily be applicable to this product.

NCBI GI #
33695104
NCBI GeneID
8477
NCBI Accession #
NP_003599.2
UniProt Primary Accession #
Q8IYL9
UniProt Secondary Accession #
O75819
UniProt Related Accession #
Q8IYL9
GenBank Nucleotide #
NM_003608.3
NCBI Official Full Name
psychosine receptor
NCBI Official Synonym Full Names
G protein-coupled receptor 65
NCBI Official Symbol
GPR65
NCBI Official Synonym Symbols
TDAG8; hTDAG8
UniProt Protein Name
Psychosine receptor
UniProt Synonym Protein Names
G-protein coupled receptor 65; T-cell death-associated gene 8 protein
Protein Family
Psychosine receptor
UniProt Gene Name
GPR65
UniProt Synonym Gene Names
TDAG8
UniProt Entry Name
PSYR_HUMAN
NCBI Protein Information
psychosine receptor; G-protein coupled receptor 65; T-cell death-associated gene 8 protein

UniProt Comments for GPR65

GPR65: Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. May have a role in activation- induced cell death or differentiation of T-cells. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 14q31-q32.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; apoptosis; multicellular organismal development; positive regulation of stress fiber formation; actin cytoskeleton reorganization; positive regulation of cAMP biosynthetic process; immune response; response to acidity
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Research Articles on GPR65

1. TDAG8 expression is decreased by more than 50%.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GPR65 antibody

 Reference Product  PubMed Publications
 GPR68 antibody  >10 publications with GPR65 and GPR68

Pathways associated with anti-GPR65 antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Metabolic Disorders Of Biological Oxidation Enzymes Pathway antibodies  Metabolic Disorders Of Biological Oxidation Enzymes Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram

Diseases associated with anti-GPR65 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >6 publications with GPR65 and Inflammation
 Disease Models, Animal Antibodies  >4 publications with GPR65 and Disease Models, Animal
 Lung Diseases Antibodies  >3 publications with GPR65 and Lung Diseases
 Pneumonia Antibodies  >1 publications with GPR65 and Pneumonia
 Respiratory Hypersensitivity Antibodies  >1 publications with GPR65 and Respiratory Hypersensitivity
 Lung Neoplasms Antibodies  >1 publications with GPR65 and Lung Neoplasms
 Liver Diseases Antibodies  >1 publications with GPR65 and Liver Diseases

Organs/Tissues associated with anti-GPR65 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >14 publications with GPR65 and Blood
 Lung Antibodies  >5 publications with GPR65 and Lung
 Spleen Antibodies  >3 publications with GPR65 and Spleen
 Lymph Node Antibodies  >2 publications with GPR65 and Lymph Node
 Thyroid Antibodies  >1 publications with GPR65 and Thyroid
 Thymus Antibodies  >1 publications with GPR65 and Thymus
 Kidney Antibodies  >1 publications with GPR65 and Kidney
 Vascular Antibodies  >1 publications with GPR65 and Vascular
 Adipose Tissue Antibodies  >1 publications with GPR65 and Adipose Tissue
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