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CRKL Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243869

MBS243869 is an antibody targeting CRKL. Also known as Goat Polyclonal to Human CRKL. Reactive in Bat, Gibbon, Chicken, Gorilla, Human, MonkeyPredicted Reactivity: Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit, Xenopus (at least 90% immunogen sequence… More details

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Product Overview

Product Name
CRKL, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human CRKL
Product Gene Name
anti-CRKL antibody
Product Synonym Names
Anti-CRKL Antibody (C-Terminus) IHC-plus; CRKL; Crk-like protein; Human CRKL
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bat, Gibbon, Chicken, Gorilla, Human, Monkey
Predicted Reactivity: Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit, Xenopus (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human CRKL.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
33,777 Da
OMIM
602007
3D Structure
ModBase 3D Structure for P46109
Target Species
Human
Immunogen Description
Synthetic peptide C-KIFDPQNPDENE from the C-terminus of human CRKL (NP_005198.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Turkey, Chicken (100%).
Immunogen Type
Synthetic peptide
Immunogen
CRKL antibody was raised against synthetic peptide C-KIFDPQNPDENE from the C-terminus of human CRKL (NP_005198.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Turkey, Chicken (100%); Mouse, Rat, Hamster, Elephant, Panda, Dog, Horse, Rabbit, Pig, Xenopus (92%); Bovine, Platypus, Zebrafish (83%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-CRKL antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CRKL antibody
ELISA (1:16000), IHC-P (2.5 - 3.75 ug/ml), WB (0.01 - 0.03 ug/ml)

Immunohistochemistry (IHC) of anti-CRKL antibody

Anti-CRKL antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Goat Anti-CRKL Polyclonal Antibody – Cat# MBS243869

Western Blot (WB) of anti-CRKL antibody

(0.01 ug/ml) Staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-CRKL Polyclonal Antibody, Western Blot (WB) – Cat# MBS243869

Other Notes

Small volumes of anti-CRKL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CRKL. It may not necessarily be applicable to this product.

NCBI GI #
4885153
NCBI GeneID
1399
NCBI Accession #
NP_005198.1
UniProt Primary Accession #
P46109
UniProt Secondary Accession #
A8KA44; D3DX35
UniProt Related Accession #
P46109
GenBank Nucleotide #
NM_005207.3
NCBI Official Full Name
crk-like protein
NCBI Official Synonym Full Names
v-crk avian sarcoma virus CT10 oncogene homolog-like
NCBI Official Symbol
CRKL
UniProt Protein Name
Crk-like protein
Protein Family
Crk-like protein
UniProt Gene Name
CRKL
UniProt Entry Name
CRKL_HUMAN
NCBI Protein Information
crk-like protein

NCBI Summary for CRKL

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]
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UniProt Comments for CRKL

CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; endosome

Molecular Function: protein binding; signal transducer activity; SH3/SH2 adaptor activity

Biological Process: anterior/posterior pattern formation; blood vessel development; organ morphogenesis; activation of MAPKK activity; nerve growth factor receptor signaling pathway; thymus development; heart development; Ras protein signal transduction; JNK cascade; parathyroid gland development
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Research Articles on CRKL

1. results suggest that CRKL has the ability to regulate colon cancer malignancy and CRKL has the potential to serve as a diagnosis and prognosis marker and a therapy target of colon cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CRKL antibody

 Reference Product  PubMed Publications
 BCR antibody  >161 publications with CRKL and BCR
 CBL antibody  >70 publications with CRKL and CBL
 ABL1 antibody  >10 publications with CRKL and ABL1
 BCAR1 antibody  >9 publications with CRKL and BCAR1
 PXN antibody  >9 publications with CRKL and PXN
 GAB2 antibody  >7 publications with CRKL and GAB2
 EPOR antibody  >6 publications with CRKL and EPOR
 CBLB antibody  >2 publications with CRKL and CBLB
 MAP4K1 antibody  >1 publications with CRKL and MAP4K1

Pathways associated with anti-CRKL antibody

 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram

Diseases associated with anti-CRKL antibody

 Disease Name  Pubmed Publications
 Leukemia, Myelogenous, Chronic, BCR-ABL Positive Antibodies  >87 publications with CRKL and Leukemia, Myelogenous, Chronic, BCR-ABL Positive
 Leukemia, Myeloid, Acute Antibodies  >13 publications with CRKL and Leukemia, Myeloid, Acute
 Lung Neoplasms Antibodies  >13 publications with CRKL and Lung Neoplasms
 Craniofacial Abnormalities Antibodies  >12 publications with CRKL and Craniofacial Abnormalities
 Disease Models, Animal Antibodies  >12 publications with CRKL and Disease Models, Animal
 DiGeorge Syndrome Antibodies  >11 publications with CRKL and DiGeorge Syndrome
 Breast Neoplasms Antibodies  >8 publications with CRKL and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >7 publications with CRKL and Neoplasms, Experimental
 Skin Diseases Antibodies  >5 publications with CRKL and Skin Diseases
 Carcinoma, Hepatocellular Antibodies  >5 publications with CRKL and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-CRKL antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >101 publications with CRKL and Blood
 Bone Antibodies  >25 publications with CRKL and Bone
 Bone Marrow Antibodies  >21 publications with CRKL and Bone Marrow
 Lung Antibodies  >20 publications with CRKL and Lung
 Nerve Antibodies  >20 publications with CRKL and Nerve
 Brain Antibodies  >19 publications with CRKL and Brain
 Embryonic Tissue Antibodies  >12 publications with CRKL and Embryonic Tissue
 Heart Antibodies  >12 publications with CRKL and Heart
 Stomach Antibodies  >7 publications with CRKL and Stomach
 Lymph Node Antibodies  >6 publications with CRKL and Lymph Node
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