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API5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS243924

MBS243924 is an antibody targeting API5. Also known as Rabbit Polyclonal (IgG) to Human API5. Reactive in Human, Mouse. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
API5, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human API5
Product Gene Name
Product Synonym Names
Anti-API5 Antibody (aa421-470) IHC-plus; API5; AAC11; AAC-11; Antiapoptosis clone 11 protein; API-5; Apoptosis inhibitor 5; FIF; API5L1; Protein XAGL; Human API5
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
API-5 Antibody detects endogenous levels of total API-5 protein.
Preparation and Storage
Store at -20 degree C for up to one year.
Molecular Weight
50,740 Da
OMIM
609774
3D Structure
ModBase 3D Structure for Q9BZZ5
Target Species
Human
Immunogen Description
Synthetic peptide from human API-5 (aa421-470).
Immunogen
API5 antibody was raised against synthetic peptide from human API-5 (aa421-470).
Antigen Modification
aa421-470
Applications Tested/Suitable for anti-API5 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-API5 antibody
ELISA (1:20000), IHC-P (5 ug/ml), WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-API5 antibody

Anti-API5 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Rabbit Anti-API5 Polyclonal Antibody – Cat# MBS243924

Western Blot (WB) of anti-API5 antibody

Western blot of extracts from 293/Jurkat/HUVEC cells, using API-5 Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-API5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS243924

Other Notes

Small volumes of anti-API5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for API5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
apoptosis inhibitor 5 isoform b
NCBI Official Synonym Full Names
apoptosis inhibitor 5
NCBI Official Symbol
API5
NCBI Official Synonym Symbols
AAC11; AAC-11
UniProt Protein Name
Apoptosis inhibitor 5
UniProt Synonym Protein Names
Antiapoptosis clone 11 protein; AAC-11; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; FIF; Protein XAGL
Protein Family
UniProt Gene Name
API5
UniProt Synonym Gene Names
API-5; AAC-11; FIF
UniProt Entry Name
API5_HUMAN
NCBI Protein Information
apoptosis inhibitor 5; FIF; antiapoptosis clone 11 protein; migration-inducing protein MIG8; cell migration-inducing gene 8 protein; fibroblast growth factor 2-interacting factor 2

NCBI Summary for API5

This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
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UniProt Comments for API5

API5: Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Belongs to the API5 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Apoptosis

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; spliceosome; membrane; cytoplasm; nucleus

Molecular Function: protein binding; fibroblast growth factor binding

Biological Process: apoptosis; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-API5 antibody

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