Chicken Anti-VIM Polyclonal Antibody – Cat# MBS244141

Anti-Vimentin antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Vimentin (VIM) Antibody – Chicken Polyclonal

Name: VIM Antibody; Polyclonal; Chicken; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS244141
Price: 625 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244141

Vimentin (VIM) is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. Vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Vimentin, Polyclonal Antibody

Full Product Name

Chicken Polyclonal (IgY) to Human Vimentin

Product Gene Name

anti-VIM antibody

Product Synonym Names

Anti-Vimentin Antibody IHC-plus; Vimentin; Human Vimentin

Clonality

Polyclonal

Isotype

IgY

Host

Chicken

Species Reactivity

Human, Mouse

Form/Format

PBS, pH 7.2, 0.02% sodium azide

Purity/Purification

Immunoaffinity Purified

Concentration

0.1 mg/ml (lot specific)

Specificity

Recognizes human and mouse Vimentin.

Preparation and Storage

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Molecular Weight

53,652 Da

OMIM

phenotype 193060

3D Structure

ModBase 3D Structure for P08670

Target Species

Human

Immunogen Description

Peptide corresponded to a region of the Vimentin gene product shared between the mouse (NP_035831, NCBI) and human (NP_003371, NCBI) gene products.

Immunogen Type

Synthetic peptide

Immunogen

Vimentin antibody was raised against peptide corresponded to a region of the Vimentin gene product shared between the mouse (NP_035831, NCBI) and human (NP_003371, NCBI) gene products.

Applications Tested/Suitable for anti-VIM antibody

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-VIM antibody

ICC (1:100 - 1:200), IHC-P (1:200), WB (1:250 - 1:500)
Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:250-1:500. Immunohistochemistry: 1:100-1:200. Immunocytochemistry: 1:100-1:200.

Other Notes

Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VIM purchase

MBS244141 is an antibody targeting VIM. Also known as Chicken Polyclonal (IgY) to Human Vimentin. Reactive in Human, Mouse. Host: Chicken.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #

62414289

NCBI GeneID

7431

NCBI Accession #

NP_003371.2

UniProt Primary Accession #

P08670

UniProt Secondary Accession #

Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4

UniProt Related Accession #

P08670

GenBank Nucleotide #

NM_003380.3

NCBI Official Full Name

vimentin

NCBI Official Synonym Full Names

vimentin

NCBI Official Symbol

VIM

NCBI Official Synonym Symbols

HEL113; CTRCT30

UniProt Protein Name

Vimentin

Protein Family

Vimentin

UniProt Gene Name

VIM

UniProt Entry Name

VIME_HUMAN

NCBI Protein Information

vimentin; epididymis luminal protein 113

NCBI Summary for VIM

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

UniProt Comments for VIM

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; leading edge; cytoplasm; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30

Research Articles on VIM

1. In the doxorubicin resistant MCF-7 cells, high levels of vimentin expression were observed.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-VIM antibody

Products by Pathway	Pathway Diagram
Alpha6-Beta4 Integrin Signaling Pathway antibodies	Alpha6-Beta4 Integrin Signaling Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptotic Cleavage Of Cellular Proteins Pathway antibodies	Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
Apoptotic Execution Phase Pathway antibodies	Apoptotic Execution Phase Pathway Diagram
Aurora B Signaling Pathway antibodies	Aurora B Signaling Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies	Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
MicroRNAs In Cancer Pathway antibodies	MicroRNAs In Cancer Pathway Diagram

Diseases associated with anti-VIM antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>418 publications with VIM and Nervous System Diseases
Neurodegenerative Diseases Antibodies	>177 publications with VIM and Neurodegenerative Diseases
Inflammation Antibodies	>53 publications with VIM and Inflammation
Kidney Diseases Antibodies	>41 publications with VIM and Kidney Diseases
Pain Antibodies	>37 publications with VIM and Pain
Disease Models, Animal Antibodies	>36 publications with VIM and Disease Models, Animal
Hemorrhage Antibodies	>34 publications with VIM and Hemorrhage
Liver Diseases Antibodies	>34 publications with VIM and Liver Diseases
Neoplasm Metastasis Antibodies	>32 publications with VIM and Neoplasm Metastasis
Breast Neoplasms Antibodies	>29 publications with VIM and Breast Neoplasms

Organs/Tissues associated with anti-VIM antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>504 publications with VIM and Brain
Blood Antibodies	>217 publications with VIM and Blood
Muscle Antibodies	>116 publications with VIM and Muscle
Nerve Antibodies	>83 publications with VIM and Nerve
Bone Antibodies	>77 publications with VIM and Bone
Kidney Antibodies	>60 publications with VIM and Kidney
Liver Antibodies	>58 publications with VIM and Liver
Bone Marrow Antibodies	>52 publications with VIM and Bone Marrow
Eye Antibodies	>48 publications with VIM and Eye
Ganglia Antibodies	>41 publications with VIM and Ganglia