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MARCH6 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244441

MBS244441 is an antibody targeting MARCH6. Also known as Goat Polyclonal to Human MARCH6 / DOA10. Reactive in Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, RatPredicted Reactivity: Rabbit, Xenopus (at least… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
MARCH6 / DOA10, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human MARCH6 / DOA10
Product Gene Name
Product Synonym Gene Name
DOA10
Product Synonym Names
Anti-MARCH6 / DOA10 Antibody (aa886-898) IHC-plus; MARCH6; DOA10; Doa10 homolog; KIAA0597; RING finger protein 176; TEB4; MARCH-VI; Protein TEB-4; RNF176; Human MARCH6; DOA10
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rat
Predicted Reactivity: Rabbit, Xenopus (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human MARCH6.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
90,413 Da
OMIM
613297
3D Structure
ModBase 3D Structure for O60337
Target Species
Human
Immunogen Description
Synthetic peptide C-QRLVNYERKSGKQ from an internal region of human MARCH6 (NP_005876.2).
Immunogen Type
Synthetic peptide
Immunogen
MARCH6 / DOA10 antibody was raised against synthetic peptide C-QRLVNYERKSGKQ from an internal region of human MARCH6 (NP_005876.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus (100%); Elephant, Rabbit, Lizard, Xenopus (92%); Pufferfish, Zebrafish (85%).
Antigen Modification
aa886-898
Applications Tested/Suitable for anti-MARCH6 antibody
Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
Application Notes for anti-MARCH6 antibody
ELISA (1:16000), IHC-P (3.75 ug/ml)

Immunohistochemistry (IHC) of anti-MARCH6 antibody

Anti-MARCH6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Goat Anti-MARCH6 Polyclonal Antibody – Cat# MBS244441

Other Notes

Small volumes of anti-MARCH6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MARCH6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
E3 ubiquitin-protein ligase MARCH6 isoform 1
NCBI Official Synonym Full Names
membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase
NCBI Official Symbol
MARCH6
NCBI Official Synonym Symbols
TEB4; DOA10; RNF176; MARCH-VI
UniProt Protein Name
E3 ubiquitin-protein ligase MARCH6
UniProt Synonym Protein Names
Doa10 homolog; Membrane-associated RING finger protein 6; Membrane-associated RING-CH protein VI; MARCH-VI; Protein TEB-4; RING finger protein 176
UniProt Gene Name
MARCH6
UniProt Synonym Gene Names
KIAA0597; RNF176; TEB4; MARCH-VI
UniProt Entry Name
MARH6_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase MARCH6; doa10 homolog; RING finger protein 176; membrane-associated RING-CH protein VI; membrane-associated RING finger protein 6; membrane-associated RING-CH finger protein 6

NCBI Summary for MARCH6

This gene encodes a member of a family of membrane-associated E3 ubiquitin ligases containing RING-CH-type zinc finger motifs. Ubiquitination of type II deiodinase by the encoded protein is an important regulatory step in thyroid hormone signalling. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
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UniProt Comments for MARCH6

MARCH6: E3 ubiquitin-protein ligase that promotes ubiquitination of DIO2, leading to its degradation. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1.

Protein type: Membrane protein, multi-pass; Ubiquitin conjugating system; Membrane protein, integral; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Ligase

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: membrane; integral to endoplasmic reticulum membrane

Molecular Function: enzyme binding; ubiquitin conjugating enzyme binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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