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PPIF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244442

MBS244442 is an antibody targeting PPIF. Also known as Rabbit Polyclonal (IgG) to Human PPIF / Cyclophilin F. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PPIF / Cyclophilin F, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human PPIF / Cyclophilin F
Product Gene Name
Product Synonym Names
Anti-PPIF / Cyclophilin F Antibody (aa86-135) IHC-plus; Cyclophilin 3; CYP3; Cyp-D; HCyP3; Peptidylprolyl isomerase F; Rotamase F; PPIase F; Cyclophilin D; Cyclophilin F; Human PPIF; Cyclophilin F
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
PPIF Antibody detects endogenous levels of total PPIF protein.
Preparation and Storage
Store at -20 degree C for up to one year.
Molecular Weight
16,541 Da
OMIM
604486
3D Structure
ModBase 3D Structure for P30405
Target Species
Human
Immunogen Description
Synthetic peptide from human PPIF (aa86-135).
Immunogen
PPIF / Cyclophilin F antibody was raised against synthetic peptide from human PPIF (aa86-135).
Antigen Modification
aa86-135
Applications Tested/Suitable for anti-PPIF antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PPIF antibody
ELISA (1:20000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-PPIF antibody

Anti-PPIF / Cyclophilin F antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-PPIF Polyclonal Antibody – Cat# MBS244442

Western Blot (WB) of anti-PPIF antibody

Western blot of extracts from HepG2/HeLa/293 cells, using PPIF Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-PPIF Polyclonal Antibody, Western Blot (WB) – Cat# MBS244442

Other Notes

Small volumes of anti-PPIF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPIF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase F, mitochondrial
NCBI Official Synonym Full Names
peptidylprolyl isomerase F
NCBI Official Symbol
PPIF
NCBI Official Synonym Symbols
CYP3; CypD; CyP-M; Cyp-D
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase F, mitochondrial
UniProt Synonym Protein Names
Cyclophilin D; CyP-D; CypD; Cyclophilin F; Mitochondrial cyclophilin; CyP-M; Rotamase F
UniProt Gene Name
PPIF
UniProt Synonym Gene Names
CYP3; PPIase F; CyP-D; CypD; CyP-M
UniProt Entry Name
PPIF_HUMAN
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase F, mitochondrial; PPIase F; rotamase F; cyclophilin 3; cyclophilin D; cyclophilin F; mitochondrial cyclophilin; peptidyl-prolyl cis-trans isomerase, mitochondrial

NCBI Summary for PPIF

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]
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UniProt Comments for PPIF

PPIF: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family.

Protein type: EC 5.2.1.8; Isomerase; Mitochondrial

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; cyclosporin A binding

Biological Process: apoptotic mitochondrial changes; protein peptidyl-prolyl isomerization; protein folding; regulation of mitochondrial membrane permeability; negative regulation of ATPase activity; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPIF antibody

 Reference Product  PubMed Publications
 LAMA2 antibody  >1 publications with PPIF and LAMA2
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