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THEM2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244503

MBS244503 is an antibody targeting THEM2. Also known as Rabbit Polyclonal (IgG) to Human THEM2 / ACOT13. Reactive in Chimpanzee, Human, Orangutan, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
THEM2 / ACOT13, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human THEM2 / ACOT13
Product Gene Name
Product Synonym Gene Name
ACOT13
Product Synonym Names
Anti-THEM2 / ACOT13 Antibody (aa100-150) IHC-plus; ACOT13; Acyl-CoA thioesterase 13; HT012; PNAS-27; Hypothalamus protein HT012; THEM2; Human THEM2; ACOT13
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Chimpanzee, Human, Orangutan, Rat
Form/Format
PBS, 0.05% BSA, 0.05% sodium azide
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human ACOT13 / THEM2
Preparation and Storage
Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.
Molecular Weight
12,366 Da
OMIM
615652
3D Structure
ModBase 3D Structure for Q9NPJ3
Target Species
Human
Immunogen Description
Amino acids 100-150 of human THEM2.
Immunogen Type
Synthetic peptide
Immunogen
THEM2 / ACOT13 antibody was raised against amino acids 100-150 of human THEM2.
Antigen Modification
aa100-150
Applications Tested/Suitable for anti-THEM2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-THEM2 antibody
IHC-P (10 ug/ml), WB (0.05 - 0.1 ug/ml)

Immunohistochemistry (IHC) of anti-THEM2 antibody

Anti-THEM2 / ACOT13 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Rabbit Anti-THEM2 Polyclonal Antibody – Cat# MBS244503

Western Blot (WB) of anti-THEM2 antibody

Western blot of THEM2 in 293 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW cell lysate using at 0.05 ug/ml.
Rabbit Anti-THEM2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS244503

Other Notes

Small volumes of anti-THEM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for THEM2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
acyl-coenzyme A thioesterase 13 isoform 1
NCBI Official Synonym Full Names
acyl-CoA thioesterase 13
NCBI Official Symbol
ACOT13
NCBI Official Synonym Symbols
HT012; THEM2; PNAS-27
UniProt Protein Name
Acyl-coenzyme A thioesterase 13
UniProt Synonym Protein Names
Thioesterase superfamily member 2Acyl-coenzyme A thioesterase 13, N-terminally processed
UniProt Gene Name
ACOT13
UniProt Synonym Gene Names
THEM2; Acyl-CoA thioesterase 13
UniProt Entry Name
ACO13_HUMAN
NCBI Protein Information
acyl-coenzyme A thioesterase 13; hypothalamus protein HT012; thioesterase superfamily member 2

NCBI Summary for THEM2

This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2009]
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UniProt Comments for THEM2

THEM2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydrohyphenylacetyl-CoA (in vitro). Belongs to the thioesterase PaaI family.

Protein type: EC 3.1.2.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: mitochondrion; spindle; nucleus; cytosol

Molecular Function: acyl-CoA hydrolase activity

Biological Process: metabolic process; protein homotetramerization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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