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Leukotriene B4 Receptor / BLT1 (LTB4R) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244534

BLT1 is a Chemoattractant Receptor that binds the potent chemoattractant leukotriene B4 (LTB4) which is involved in defense against infection. Leukotriene B4 also activates the transcription factor PPAR alpha, resulting in the activation of genes that… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Leukotriene B4 Receptor / BLT1 (LTB4R), Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human Leukotriene B4 Receptor / BLT1
Product Gene Name
anti-LTB4R antibody
Product Synonym Gene Name
BLT1
Product Synonym Names
Anti-Leukotriene B4 Receptor / BLT1 Antibody (Cytoplasmic Domain) IHC-plus; BLT; BLTR; Blt-1; Chemokine receptor-like 1; G protein-coupled receptor 16; GPR16; Leukotriene B4 receptor; Leukotriene B4 receptor 1; G-protein coupled receptor 16; LTBR1; p2Y purinoceptor 7; p2Y7; LTB4-R 1; p2RY7; CMKRL1; LTB4-R1; LTB4R1; Human Leukotriene B4 Receptor; BLT1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Predicted Reactivity: Monkey, Mouse, Rat, Hamster, Rabbit
Form/Format
PBS, 0.1% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human Leukotriene B4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Preparation and Storage
Aliquot and store undiluted at -20°C or below for up to 1 year.
Can be stored undiluted at 4°C for up one month.
Avoid freeze/thaw cycles.
OMIM
601531
3D Structure
ModBase 3D Structure for Q15722
Immunogen Species
Raised against human
Antigen Type
Synthetic peptide
Immunogen
Leukotriene B4 Receptor / BLT1 antibody was raised against synthetic 20 amino acid peptide from 1st cytoplasmic domain of human Leukotriene B4 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Rat, Rabbit (95%); Monkey, Mouse, Hamster (90%).
Epitope
Cytoplasmic Domain
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-LTB4R antibody

Family: GPCR; Subfamily: Chemoattractant
Applications Tested/Suitable for anti-LTB4R antibody
Immunohistochemistry Paraffin (IHC-P)
Application Notes for anti-LTB4R antibody
IHC-P (20 ug/ml)
Optimal dilutions/concentrations should be determined by the end user.

Immunohistochemistry (IHC) of anti-LTB4R antibody

Anti-Leukotriene B4 Receptor antibody IHC of human spleen, red pulp. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-LTB4R Polyclonal Antibody – Cat# MBS244534

Other Notes

Small volumes of anti-LTB4R antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LTB4R purchase

MBS244534 is an antibody targeting LTB4R. Also known as Rabbit Polyclonal to Human Leukotriene B4 Receptor / BLT1. Reactive in Human
Predicted Reactivity: Monkey, Mouse, Rat, Hamster, Rabbit. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LTB4R. It may not necessarily be applicable to this product.

NCBI GI #
221218998
NCBI GeneID
1241
NCBI Accession #
NP_001137391.1
UniProt Primary Accession #
Q15722
UniProt Secondary Accession #
Q13305; Q53XV5; Q92641; Q9BSU5
UniProt Related Accession #
Q15722
GenBank Nucleotide #
NM_001143919.2
NCBI Official Full Name
leukotriene B4 receptor 1
NCBI Official Synonym Full Names
leukotriene B4 receptor
NCBI Official Symbol
LTB4R
NCBI Official Synonym Symbols
BLT1; BLTR; P2Y7; GPR16; LTBR1; P2RY7; CMKRL1; LTB4R1
UniProt Protein Name
Leukotriene B4 receptor 1
UniProt Synonym Protein Names
Chemoattractant receptor-like 1; G-protein coupled receptor 16; P2Y purinoceptor 7; P2Y7
Protein Family
Leukotriene B4 receptor
UniProt Gene Name
LTB4R
UniProt Synonym Gene Names
BLT; BLT1; BLTR; CMKRL1; GPR16; P2RY7; LTB4-R 1; LTB4-R1; P2Y7
UniProt Entry Name
LT4R1_HUMAN
NCBI Protein Information
leukotriene B4 receptor 1; LTB4-R1; LTB4-R 1; P2Y purinoceptor 7; chemokine receptor-like 1; G protein-coupled receptor 16; G-protein coupled receptor 16; chemoattractant receptor-like 1; purinergic receptor P2Y, G-protein coupled, 7

UniProt Comments for LTB4R

LTB4R: Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 14q11.2-q12

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: leukotriene B4 receptor activity; nucleotide binding; leukotriene receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; muscle contraction; immune response; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); cell motility; inflammatory response
Show more

Research Articles on LTB4R

1. we show that orchestrated action of CXCR2 and leukotriene B4 receptor BLT1 plays a key role in neutrophil recruitment during the development of imiquimod (IMQ)-induced psoriatic skin lesions in mice

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LTB4R antibody

 Reference Product  PubMed Publications
 CYSLTR2 antibody  >1 publications with LTB4R and CYSLTR2
 ADRBK1 antibody  >1 publications with LTB4R and ADRBK1
 CCK antibody  >1 publications with LTB4R and CCK

Pathways associated with anti-LTB4R antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eicosanoid Ligand-binding Receptors Pathway antibodies  Eicosanoid Ligand-binding Receptors Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Leukotriene Receptors Pathway antibodies  Leukotriene Receptors Pathway Diagram

Diseases associated with anti-LTB4R antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >34 publications with LTB4R and Inflammation
 Asthma Antibodies  >16 publications with LTB4R and Asthma
 Necrosis Antibodies  >8 publications with LTB4R and Necrosis
 Pancreatic Neoplasms Antibodies  >2 publications with LTB4R and Pancreatic Neoplasms
 Leukemia, Myeloid Antibodies  >2 publications with LTB4R and Leukemia, Myeloid
 Fibrosis Antibodies  >2 publications with LTB4R and Fibrosis
 Disease Models, Animal Antibodies  >2 publications with LTB4R and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >2 publications with LTB4R and Neoplasms, Experimental
 Leukemia Antibodies  >2 publications with LTB4R and Leukemia
 Bronchial Hyperreactivity Antibodies  >1 publications with LTB4R and Bronchial Hyperreactivity

Organs/Tissues associated with anti-LTB4R antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >47 publications with LTB4R and Blood
 Lung Antibodies  >14 publications with LTB4R and Lung
 Skin Antibodies  >5 publications with LTB4R and Skin
 Bone Marrow Antibodies  >5 publications with LTB4R and Bone Marrow
 Kidney Antibodies  >3 publications with LTB4R and Kidney
 Spleen Antibodies  >2 publications with LTB4R and Spleen
 Uterus Antibodies  >2 publications with LTB4R and Uterus
 Eye Antibodies  >1 publications with LTB4R and Eye
 Ovary Antibodies  >1 publications with LTB4R and Ovary
 Brain Antibodies  >1 publications with LTB4R and Brain
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