• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

IDH3A Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244547

MBS244547 is an antibody targeting IDH3A. Also known as Goat Polyclonal to Human IDH3A. Reactive in Gibbon, Bovine, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, RatPredicted Reactivity: Gorilla, Dog (at least 90% immunogen sequence identity).… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
IDH3A, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human IDH3A
Product Gene Name
anti-IDH3A antibody
Product Synonym Names
Anti-IDH3A Antibody (C-Terminus) IHC-plus; IDH3A; H-IDH alpha; NAD+-specific ICDH; Human IDH3A
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Bovine, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat
Predicted Reactivity: Gorilla, Dog (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human IDH3A.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
31,381 Da
OMIM
601149
3D Structure
ModBase 3D Structure for P50213
Target Species
Human
Immunogen Description
Synthetic peptide DFTEEICRRVKDLD from the C-terminus of human IDH3A (NP_005521.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Horse, Rabbit, Pig, Opossum (100%).
Immunogen Type
Synthetic peptide
Immunogen
IDH3A antibody was raised against synthetic peptide DFTEEICRRVKDLD from the C-terminus of human IDH3A (NP_005521.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Horse, Rabbit, Pig, Opossum (100%); Gorilla, Panda, Dog (93%); Platypus (86%).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-IDH3A antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-IDH3A antibody
ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 37kD band observed in lysates of Human Lymph Nodes, and of cell line DAUDI (calculated MW of 39.6kD according to NP_005521.1). Recommended concentration: 0.1-0.3 ug/ml.

Immunohistochemistry (IHC) of anti-IDH3A antibody

Anti-IDH3A antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Goat Anti-IDH3A Polyclonal Antibody – Cat# MBS244547

Western Blot (WB) of anti-IDH3A antibody

IDH3A antibody (0.1 ug/ml) staining of Human Lymph Nodes lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-IDH3A Polyclonal Antibody, Western Blot (WB) – Cat# MBS244547

Other Notes

Small volumes of anti-IDH3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IDH3A. It may not necessarily be applicable to this product.

NCBI GI #
5031777
NCBI GeneID
3419
NCBI Accession #
NP_005521.1
UniProt Primary Accession #
P50213
UniProt Secondary Accession #
Q9H3X0; D3DW83
UniProt Related Accession #
P50213
GenBank Nucleotide #
NM_005530.2
NCBI Official Full Name
isocitrate dehydrogenase
NCBI Official Synonym Full Names
isocitrate dehydrogenase 3 (NAD+) alpha
NCBI Official Symbol
IDH3A
UniProt Protein Name
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
UniProt Synonym Protein Names
Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha
Protein Family
Isocitrate dehydrogenase
UniProt Gene Name
IDH3A
UniProt Entry Name
IDH3A_HUMAN
NCBI Protein Information
isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; H-IDH alpha; NAD+-specific ICDH; NAD(+)-specific ICDH subunit alpha; isocitric dehydrogenase subunit alpha; isocitrate dehydrogenase (NAD+) alpha chain; NAD(H)-specific isocitrate dehydrogenase alpha subunit

NCBI Summary for IDH3A

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for IDH3A

IDH3A: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 has apparent tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; EC 1.1.1.41; Mitochondrial

Chromosomal Location of Human Ortholog: 15q25.1-q25.2

Cellular Component: mitochondrion; mitochondrial matrix; nucleus

Molecular Function: magnesium ion binding; isocitrate dehydrogenase (NAD+) activity; NAD binding

Biological Process: cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process
Show more

Research Articles on IDH3A

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IDH3A antibody

 Reference Product  PubMed Publications
 IDH2 antibody  >2 publications with IDH3A and IDH2
 OGDH antibody  >1 publications with IDH3A and OGDH
 DLST antibody  >1 publications with IDH3A and DLST
 IDH3B antibody  >1 publications with IDH3A and IDH3B

Pathways associated with anti-IDH3A antibody

 Products by Pathway  Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram

Diseases associated with anti-IDH3A antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with IDH3A and Nervous System Diseases
 Cognition Disorders Antibodies  >1 publications with IDH3A and Cognition Disorders
 Cardiovascular Diseases Antibodies  >1 publications with IDH3A and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >1 publications with IDH3A and Breast Neoplasms
 Disease Models, Animal Antibodies  >1 publications with IDH3A and Disease Models, Animal
 Inflammation Antibodies  >1 publications with IDH3A and Inflammation
 Fatty Liver Antibodies  >1 publications with IDH3A and Fatty Liver

Organs/Tissues associated with anti-IDH3A antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >3 publications with IDH3A and Kidney
 Blood Antibodies  >2 publications with IDH3A and Blood
 Liver Antibodies  >2 publications with IDH3A and Liver
 Brain Antibodies  >2 publications with IDH3A and Brain
 Adipose Tissue Antibodies  >1 publications with IDH3A and Adipose Tissue
 Bone Antibodies  >1 publications with IDH3A and Bone
 Thyroid Antibodies  >1 publications with IDH3A and Thyroid
 Vascular Antibodies  >1 publications with IDH3A and Vascular
 Lung Antibodies  >1 publications with IDH3A and Lung
 Heart Antibodies  >1 publications with IDH3A and Heart
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000