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HSP40 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244565

MBS244565 is an antibody targeting HSP40. Also known as Rabbit Polyclonal to Human HSP40. Reactive in Beluga, Bovine, Chicken, Dog, Fish, Xenopus, Guinea pig, Hamster, Human, Monkey, Mouse, Mussel, Pig, Rabbit, Rat, Sheep, Scallop. Host: Rabbit. More details

$680 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HSP40, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human HSP40
Product Gene Name
anti-HSP40 antibody
Product Synonym Names
Anti-HSP40 Antibody IHC-plus; Human HSP40
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Beluga, Bovine, Chicken, Dog, Fish, Xenopus, Guinea pig, Hamster, Human, Monkey, Mouse, Mussel, Pig, Rabbit, Rat, Sheep, Scallop
Form/Format
PBS, 0.09% azide, 50% glycerol
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at -20°C.
Target Species
Human
Target
HSP40
Immunogen
HSP40 antibody was raised against recombinant human HSP40.
Immunogen Type
Recombinant protein
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-HSP40 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-HSP40 antibody
IHC-P (10 ug/ml)
WB (1:10000)
Immunohistochemistry: (MBS244565) was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for (MBS244565) was determined to be 10 ug/ml

(Optimal dilution to be determined by the researcher)

Immunohistochemistry (IHC) of anti-HSP40 antibody

Anti-DNAJ / Hsp40 / Heat Shock Protein 40 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-HSP40 Polyclonal Antibody – Cat# MBS244565

Immunohistochemistry (IHC) of anti-HSP40 antibody

Anti-DNAJ / Hsp40 / Heat Shock Protein 40 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-HSP40 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS244565

Western Blot (WB) of anti-HSP40 antibody

Western blot of Hsp40: Lane 1: MWM, Lane 2: SPP-400 (Hsp40 recombinant protein), Lane 3: SPP-640 (DnaJ recombinant protein), Lane 4: HeLa (heat shocked) cell lysate, Lane 5:3T3 (heat shocked) cell lysate, Lane 6: PC-12 cell lysate.
Rabbit Anti-HSP40 Polyclonal Antibody, Western Blot (WB) – Cat# MBS244565

Other Notes

Small volumes of anti-HSP40 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HSP40. It may not necessarily be applicable to this product.

NCBI GI #
60592743
NCBI Accession #
BAD90846.1
NCBI Official Full Name
Hsp40
UniProt Protein Name
Hsp40
UniProt Synonym Protein Names
Hsp40
Protein Family
DnaJ homolog subfamily
UniProt Gene Name
Hsp40
UniProt Entry Name
Q5CCL5_BOMMO

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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