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MSH5 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244570

MBS244570 is an antibody targeting MSH5. Also known as Goat Polyclonal to Human MSH5. Reactive in Gibbon, Chicken, Xenopus, Human, Monkey, MousePredicted Reactivity: Rat, Bovine, Dog, Horse, Pig (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
MSH5, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human MSH5
Product Gene Name
Product Synonym Names
Anti-MSH5 Antibody (Internal) IHC-plus; MSH5; MutS homolog 5 (E Coli); HMSH5; MutS protein homolog 5; NG23; G7; MutS (E Coli) homolog 5; MUTSH5; Human MSH5
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Chicken, Xenopus, Human, Monkey, Mouse
Predicted Reactivity: Rat, Bovine, Dog, Horse, Pig (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human MSH5. This antibody is expected to recognise three reported isoforms (NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
91,862 Da
OMIM
603382
Target Species
Human
Immunogen Description
Synthetic peptide GDLHCEIRDQET from an internal region of human MSH5 (NP_079535.3 ; NP_751897.1; NP_002432.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Mouse, Chicken, Xenopus (100%); Rat, Panda, Bovine, Dog, Horse, Pig (92%).
Immunogen Type
Synthetic peptide
Immunogen
MSH5 antibody was raised against synthetic peptide GDLHCEIRDQET from an internal region of human MSH5 (NP_079535.3 ; NP_751897.1; NP_002432.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Mouse, Chicken, Xenopus (100%); Rat, Panda, Bovine, Dog, Horse, Pig (92%); Marmoset (83%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-MSH5 antibody
Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
Application Notes for anti-MSH5 antibody
ELISA (1:64000), IHC-P (2.5 - 3.75 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 38kD band in Human Heart, Tonsil and Thymus lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explana...

Immunohistochemistry (IHC) of anti-MSH5 antibody

Anti-MSH5 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Goat Anti-MSH5 Polyclonal Antibody – Cat# MBS244570

Immunohistochemistry (IHC) of anti-MSH5 antibody

Anti-MSH5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Goat Anti-MSH5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS244570

Other Notes

Small volumes of anti-MSH5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MSH5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens chromosome 6, GRCh37.p13 Primary Assembly
NCBI Official Synonym Full Names
mutS homolog 5
NCBI Official Symbol
MSH5
NCBI Official Synonym Symbols
G7; NG23; MUTSH5
UniProt Protein Name
MutS protein homolog 5
UniProt Gene Name
MSH5
UniProt Synonym Gene Names
hMSH5
UniProt Entry Name
MSH5_HUMAN
NCBI Protein Information
mutS protein homolog 5

NCBI Summary for MSH5

This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair and meiotic recombination. This protein is similar to a Saccharomyces cerevisiae protein that participates in segregation fidelity and crossing-over events during meiosis. This protein plays a role in promoting ionizing radiation-induced apoptosis. This protein forms hetero-oligomers with another member of this family, mutS homolog 4. Polymorphisms in this gene have been linked to various human diseases, including IgA deficiency, common variable immunodeficiency, and premature ovarian failure. Alternative splicing results multiple transcript variants. Read-through transcription also exists between this gene and the downstream chromosome 6 open reading frame 26 (C6orf26) gene. [provided by RefSeq, Feb 2011]
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UniProt Comments for MSH5

MSH5: Involved in meiotic recombination. Facilitate crossovers between homologs during meiosis. Belongs to the DNA mismatch repair MutS family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: synaptonemal complex

Molecular Function: DNA-dependent ATPase activity; protein binding; damaged DNA binding; ATP binding; mismatched DNA binding

Biological Process: homologous chromosome segregation; meiotic recombination; chiasma formation; meiotic mismatch repair
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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