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RNF144A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244628

MBS244628 is an antibody targeting RNF144A. Also known as Rabbit Polyclonal (IgG) to Human RNF144A / RNF144. Reactive in Human, Mouse. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
RNF144A / RNF144, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human RNF144A / RNF144
Product Gene Name
Product Synonym Gene Name
RNF144
Product Synonym Names
Anti-RNF144A / RNF144 Antibody (aa151-200) IHC-plus; RNF144A; KIAA0161; Ring finger protein 144; Ring finger protein 144A; UbcM4-interacting protein 4; RNF144; UBCE7IP4; Human RNF144A; RNF144
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
RNF144A Antibody detects endogenous levels of total RNF144A protein.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
32,890 Da
3D Structure
ModBase 3D Structure for P50876
Target Species
Human
Immunogen Description
Synthetic peptide from human RNF144A (aa151-200).
Immunogen
RNF144A / RNF144 antibody was raised against synthetic peptide from human RNF144A (aa151-200).
Antigen Modification
aa151-200
Applications Tested/Suitable for anti-RNF144A antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RNF144A antibody
ELISA (1:40000), IHC-P (1:100), WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-RNF144A antibody

Anti-RNF144A antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Rabbit Anti-RNF144A Polyclonal Antibody – Cat# MBS244628

Western Blot (WB) of anti-RNF144A antibody

Western blot of extracts from COLO cells, using RNF144A Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-RNF144A Polyclonal Antibody, Western Blot (WB) – Cat# MBS244628

Other Notes

Small volumes of anti-RNF144A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RNF144A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
probable E3 ubiquitin-protein ligase RNF144A
NCBI Official Synonym Full Names
ring finger protein 144A
NCBI Official Symbol
RNF144A
NCBI Official Synonym Symbols
RNF144; UBCE7IP4
UniProt Protein Name
E3 ubiquitin-protein ligase RNF144A
UniProt Synonym Protein Names
RING finger protein 144A; UbcM4-interacting protein 4; Ubiquitin-conjugating enzyme 7-interacting protein 4
UniProt Gene Name
RNF144A
UniProt Synonym Gene Names
KIAA0161; RNF144; UBCE7IP4
UniProt Entry Name
R144A_HUMAN
NCBI Protein Information
probable E3 ubiquitin-protein ligase RNF144A; ring finger protein 144; UbcM4-interacting protein 4; ubiquitin conjugating enzyme 7 interacting protein 4; ubiquitin-conjugating enzyme 7-interacting protein 4

NCBI Summary for RNF144A

The protein encoded by this protein contains a RING finger, a motif known to be involved in protein-DNA and protein-protein interactions. The mouse counterpart of this protein has been shown to interact with Ube2l3/UbcM4, which is an ubiquitin-conjugating enzyme involved in embryonic development. [provided by RefSeq, Jul 2008]
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UniProt Comments for RNF144A

RNF144A: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Belongs to the RBR family. RNF144 subfamily.

Protein type: Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system; Membrane protein, integral; Ligase

Chromosomal Location of Human Ortholog: 2p25.2

Cellular Component: Golgi apparatus; cytoplasmic vesicle membrane; plasma membrane; integral to membrane

Molecular Function: zinc ion binding; ligase activity

Biological Process: protein ubiquitination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RNF144A antibody

 Reference Product  PubMed Publications
 MTA1 antibody  >1 publications with RNF144A and MTA1

Organs/Tissues associated with anti-RNF144A antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with RNF144A and Blood
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