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HINT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS244644

MBS244644 is an antibody targeting HINT. Also known as Rabbit Polyclonal to Human HINT / HINT1. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
HINT / HINT1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human HINT / HINT1
Product Gene Name
Product Synonym Gene Name
HINT1
Product Synonym Names
Anti-HINT / HINT1 Antibody (Internal) IHC-plus; HINT1; HINT; NMAN; Protein kinase C inhibitor 1; PKCI-1; PKCI1; PRKCNH1; Human HINT; HINT1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
13,802 Da
OMIM
phenotype 601314
3D Structure
ModBase 3D Structure for P49773
Target Species
Human
Immunogen Description
A 17 amino acid peptide near the center of human HINT1.
Immunogen Type
Synthetic peptide
Immunogen
HINT / HINT1 antibody was raised against a 17 amino acid peptide near the center of human HINT1.
Antigen Modification
Internal
Applications Tested/Suitable for anti-HINT antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-HINT antibody
IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: HINT1 antibody can be used for detection of HINT1 by Western blot at 1-2 ug/ml

Immunohistochemistry (IHC) of anti-HINT antibody

Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-HINT Polyclonal Antibody – Cat# MBS244644

Immunocytochemistry (ICC) of anti-HINT antibody

Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.
Rabbit Anti-HINT Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS244644

Western Blot (WB) of anti-HINT antibody

Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.
Rabbit Anti-HINT Polyclonal Antibody, Western Blot (WB) – Cat# MBS244644

Other Notes

Small volumes of anti-HINT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HINT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histidine triad nucleotide-binding protein 1
NCBI Official Synonym Full Names
histidine triad nucleotide binding protein 1
NCBI Official Symbol
HINT1
NCBI Official Synonym Symbols
HINT; NMAN; PKCI-1; PRKCNH1
UniProt Protein Name
Histidine triad nucleotide-binding protein 1
UniProt Synonym Protein Names
Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; PKCI-1
UniProt Gene Name
HINT1
UniProt Synonym Gene Names
HINT; PKCI1; PRKCNH1; PKCI-1
UniProt Entry Name
HINT1_HUMAN
NCBI Protein Information
histidine triad nucleotide-binding protein 1; protein kinase C inhibitor 1; adenosine 5'-monophosphoramidase; protein kinase C-interacting protein 1

NCBI Summary for HINT

The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. This gene has been found to be a tumor suppressing gene. Several transcript variants, but only one of them protein-coding, have been found for this gene. [provided by RefSeq, Dec 2012]
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UniProt Comments for HINT

HINT1: Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha- acetyl lysine methyl ester and AMP-NH2. Belongs to the HINT family.

Protein type: Hydrolase; EC 3.-.-.-; Cytoskeletal

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: cytoskeleton; histone deacetylase complex; cytoplasm; plasma membrane; nucleus

Molecular Function: protein kinase C binding; hydrolase activity; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; purine ribonucleotide catabolic process; signal transduction

Disease: Neuromyotonia And Axonal Neuropathy, Autosomal Recessive
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HINT antibody

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